Restriction map for
pF1KB8396
(Linear) (Six-base) MAPSORT of: KIBB8396.seq Check: 2773 from: 1 to: 1392
KIBB8396.orf
With 182 enzymes: SgfI *
March 3, 2008 11:05 ..
AflIII A'CryG_T
Cuts at: 0 284 1392
Size: 284 1108
AhdI GACnn_n'nnGTC
Cuts at: 0 1191 1392
Size: 1191 201
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1010 1392
Size: 1010 382
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 271 303 1392
Size: 271 32 1089
Fragments arranged by size:
1089 271 32
ApaI G_GGCC'C
Cuts at: 0 649 693 1392
Size: 649 44 699
Fragments arranged by size:
699 649 44
AvaI C'yCGr_G
Cuts at: 0 55 132 222 447 660 693 928 1392
Size: 55 77 90 225 213 33 235 464
Fragments arranged by size:
464 235 225 213 90 77 55 33
BamHI G'GATC_C
Cuts at: 0 561 1392
Size: 561 831
BanI G'GyrC_C
Cuts at: 0 457 1392
Size: 457 935
BanII G_rGCy'C
Cuts at: 0 221 536 649 693 939 1114 1392
Size: 221 315 113 44 246 175 278
Fragments arranged by size:
315 278 246 221 175 113 44
BbeI G_GCGC'C
Cuts at: 0 461 1392
Size: 461 931
BbsI GAAGACnn'nnnn_
Cuts at: 0 567 1392
Size: 567 825
BglI GCCn_nnn'nGGC
Cuts at: 0 544 640 664 719 1392
Size: 544 96 24 55 673
Fragments arranged by size:
673 544 96 55 24
Bme1580I G_kGCm'C
Cuts at: 0 485 617 636 649 693 1392
Size: 485 132 19 13 44 699
Fragments arranged by size:
699 485 132 44 19 13
BmgBI CAC'GTC
Cuts at: 0 17 834 1069 1392
Size: 17 817 235 323
Fragments arranged by size:
817 323 235 17
BmrI ACTGGGnnnn_n'
Cuts at: 0 313 435 1392
Size: 313 122 957
Fragments arranged by size:
957 313 122
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 81 228 1392
Size: 81 147 1164
Fragments arranged by size:
1164 147 81
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 397 1186 1282 1392
Size: 319 78 789 96 110
Fragments arranged by size:
789 319 110 96 78
Bpu10I CC'TnA_GC
Cuts at: 0 778 1210 1392
Size: 778 432 182
Fragments arranged by size:
778 432 182
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 1060 1128 1392
Size: 268 792 68 264
Fragments arranged by size:
792 268 264 68
BsaAI yAC'GTr
Cuts at: 0 1014 1221 1392
Size: 1014 207 171
Fragments arranged by size:
1014 207 171
BsaHI Gr'CG_yC
Cuts at: 0 458 1060 1392
Size: 458 602 332
Fragments arranged by size:
602 458 332
BsaWI w'CCGG_w
Cuts at: 0 763 1392
Size: 763 629
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 271 301 1392
Size: 271 30 1091
Fragments arranged by size:
1091 271 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 292 852 1321 1392
Size: 292 560 469 71
Fragments arranged by size:
560 469 292 71
BseYI C'CCAG_C
Cuts at: 0 187 227 681 1392
Size: 187 40 454 711
Fragments arranged by size:
711 454 187 40
BsiEI CG_ry'CG
Cuts at: 0 275 411 507 548 656 723 1392
Size: 275 136 96 41 108 67 669
Fragments arranged by size:
669 275 136 108 96 67 41
BsiHKAI G_wGCw'C
Cuts at: 0 209 536 1313 1392
Size: 209 327 777 79
Fragments arranged by size:
777 327 209 79
Bsp1286I G_dGCh'C
Cuts at: 0 209 221 485 536 617 636 649 693
Size: 209 12 264 51 81 19 13 44
Cuts at: 693 939 1114 1313 1392
Size: 246 175 199 79
Fragments arranged by size:
264 246 209 199 175 81 79 51
44 19 13 12
BspEI T'CCGG_A
Cuts at: 0 763 1392
Size: 763 629
BsrBI CCG'CTC
Cuts at: 0 353 1074 1392
Size: 353 721 318
Fragments arranged by size:
721 353 318
BsrFI r'CCGG_y
Cuts at: 0 427 454 502 1392
Size: 427 27 48 890
Fragments arranged by size:
890 427 48 27
BssHII G'CGCG_C
Cuts at: 0 381 1392
Size: 381 1011
BstAPI GCAn_nnn'nTGC
Cuts at: 0 38 1359 1392
Size: 38 1321 33
Fragments arranged by size:
1321 38 33
BstYI r'GATC_y
Cuts at: 0 561 1392
Size: 561 831
BtgI C'CryG_G
Cuts at: 0 192 411 569 1392
Size: 192 219 158 823
Fragments arranged by size:
823 219 192 158
EaeI y'GGCC_r
Cuts at: 0 272 408 504 545 626 720 1392
Size: 272 136 96 41 81 94 672
Fragments arranged by size:
672 272 136 96 94 81 41
EagI C'GGCC_G
Cuts at: 0 272 408 504 545 720 1392
Size: 272 136 96 41 175 672
Fragments arranged by size:
672 272 175 136 96 41
EarI CTCTTCn'nnn_
Cuts at: 0 800 812 1392
Size: 800 12 580
Fragments arranged by size:
800 580 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 351 1392
Size: 351 1041
EcoICRI GAG'CTC
Cuts at: 0 534 1392
Size: 534 858
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 397 1186 1282 1392
Size: 319 78 789 96 110
Fragments arranged by size:
789 319 110 96 78
EcoO109I rG'GnC_Cy
Cuts at: 0 646 689 690 750 925 1392
Size: 646 43 1 60 175 467
Fragments arranged by size:
646 467 175 60 43 1
HaeII r_GCGC'y
Cuts at: 0 461 610 1173 1179 1392
Size: 461 149 563 6 213
Fragments arranged by size:
563 461 213 149 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1177 1209 1392
Size: 1177 32 183
Fragments arranged by size:
1177 183 32
KasI G'GCGC_C
Cuts at: 0 457 1392
Size: 457 935
MluI A'CGCG_T
Cuts at: 0 284 1392
Size: 284 1108
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 890 1392
Size: 890 502
MslI CAynn'nnrTG
Cuts at: 0 238 898 992 1392
Size: 238 660 94 400
Fragments arranged by size:
660 400 238 94
MspA1I CmG'CkG
Cuts at: 0 180 413 734 1392
Size: 180 233 321 658
Fragments arranged by size:
658 321 233 180
NaeI GCC'GGC
Cuts at: 0 456 504 1392
Size: 456 48 888
Fragments arranged by size:
888 456 48
NarI GG'CG_CC
Cuts at: 0 458 1392
Size: 458 934
NgoMIV G'CCGG_C
Cuts at: 0 454 502 1392
Size: 454 48 890
Fragments arranged by size:
890 454 48
NotI GC'GGCC_GC
Cuts at: 0 720 1392
Size: 720 672
NspI r_CATG'y
Cuts at: 0 348 1392
Size: 348 1044
PasI CC'CwG_GG
Cuts at: 0 921 1392
Size: 921 471
PfoI T'CCnGG_A
Cuts at: 0 335 1163 1392
Size: 335 828 229
Fragments arranged by size:
828 335 229
PmlI CAC'GTG
Cuts at: 0 1221 1392
Size: 1221 171
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 271 303 1392
Size: 271 32 1089
Fragments arranged by size:
1089 271 32
PpuMI rG'GwC_Cy
Cuts at: 0 925 1392
Size: 925 467
PspOMI G'GGCC_C
Cuts at: 0 645 689 1392
Size: 645 44 703
Fragments arranged by size:
703 645 44
PspXI vC'TCGA_Gb
Cuts at: 0 55 1392
Size: 55 1337
PstI C_TGCA'G
Cuts at: 0 404 1392
Size: 404 988
SacI G_AGCT'C
Cuts at: 0 536 1392
Size: 536 856
SacII CC_GC'GG
Cuts at: 0 414 1392
Size: 414 978
SanDI GG'GwC_CC
Cuts at: 0 925 1392
Size: 925 467
SfcI C'TryA_G
Cuts at: 0 70 400 1392
Size: 70 330 992
Fragments arranged by size:
992 330 70
SfoI GGC'GCC
Cuts at: 0 459 1392
Size: 459 933
SmaI CCC'GGG
Cuts at: 0 134 449 695 930 1392
Size: 134 315 246 235 462
Fragments arranged by size:
462 315 246 235 134
SmlI C'TyrA_G
Cuts at: 0 55 247 1075 1107 1392
Size: 55 192 828 32 285
Fragments arranged by size:
828 285 192 55 32
StyI C'CwwG_G
Cuts at: 0 872 1253 1392
Size: 872 381 139
Fragments arranged by size:
872 381 139
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 295 1181 1392
Size: 295 886 211
Fragments arranged by size:
886 295 211
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 331 363 888 920 1392
Size: 331 32 525 32 472
Fragments arranged by size:
525 472 331 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 835 1392
Size: 835 557
XhoI C'TCGA_G
Cuts at: 0 55 1392
Size: 55 1337
XmaI C'CCGG_G
Cuts at: 0 132 447 693 928 1392
Size: 132 315 246 235 464
Fragments arranged by size:
464 315 246 235 132
Enzymes that do cut:
AflIII AhdI AlfI AloI ApaI AvaI BamHI BanI
BanII BbeI BbsI BglI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI Bsp1286I BspEI BsrBI BsrFI BssHII
BstAPI BstYI BtgI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I HaeII Hin4I KasI MluI MmeI MslI
MspA1I NaeI NarI NgoMIV NotI NspI PasI PfoI
PmlI PpiI PpuMI PspOMI PspXI PstI SacI SacII
SanDI SfcI SfoI SmaI SmlI StyI TaqII TstI
Tth111I XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AleI AlwNI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BbvCI BcgI BciVI BclI BfrBI BglII
BlpI BmtI BsaI BsaBI BsgI BsiWI BsmI BsmBI
BspHI BspMI BsrDI BsrGI BssSI BstBI BstEII BstXI
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MscI NcoI NdeI
NheI NruI NsiI PacI PciI PflMI PmeI PshAI
PsiI PsrI PvuI PvuII RsrII SalI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI SwaI TatI XbaI XcmI XmnI
ZraI