Restriction map for
pF1KB8389
(Linear) (Six-base) MAPSORT of: KIBB8389.seq Check: 9262 from: 1 to: 1179
KIBB8389.orf
With 182 enzymes: SgfI *
March 3, 2008 11:05 ..
AccI GT'mk_AC
Cuts at: 0 41 137 1179
Size: 41 96 1042
Fragments arranged by size:
1042 96 41
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1141 1179
Size: 1141 38
AfeI AGC'GCT
Cuts at: 0 967 1179
Size: 967 212
AflIII A'CryG_T
Cuts at: 0 827 1179
Size: 827 352
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1000 1032 1179
Size: 1000 32 147
Fragments arranged by size:
1000 147 32
AlwNI CAG_nnn'CTG
Cuts at: 0 580 993 1179
Size: 580 413 186
Fragments arranged by size:
580 413 186
ApaI G_GGCC'C
Cuts at: 0 284 532 1179
Size: 284 248 647
Fragments arranged by size:
647 284 248
AvaI C'yCGr_G
Cuts at: 0 57 262 655 684 1034 1179
Size: 57 205 393 29 350 145
Fragments arranged by size:
393 350 205 145 57 29
BamHI G'GATC_C
Cuts at: 0 1145 1179
Size: 1145 34
BanI G'GyrC_C
Cuts at: 0 556 570 610 1043 1179
Size: 556 14 40 433 136
Fragments arranged by size:
556 433 136 40 14
BanII G_rGCy'C
Cuts at: 0 19 188 284 451 532 756 805 854
Size: 19 169 96 167 81 224 49 49
Cuts at: 854 1157 1179
Size: 303 22
Fragments arranged by size:
303 224 169 167 96 81 49 49
22 19
BbeI G_GCGC'C
Cuts at: 0 1047 1179
Size: 1047 132
BglI GCCn_nnn'nGGC
Cuts at: 0 168 342 511 687 736 1179
Size: 168 174 169 176 49 443
Fragments arranged by size:
443 176 174 169 168 49
Bme1580I G_kGCm'C
Cuts at: 0 284 532 559 1179
Size: 284 248 27 620
Fragments arranged by size:
620 284 248 27
BmtI G_CTAG'C
Cuts at: 0 718 1179
Size: 718 461
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 870 1179
Size: 870 309
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 546 1015 1021 1179
Size: 546 469 6 158
Fragments arranged by size:
546 469 158 6
BsaHI Gr'CG_yC
Cuts at: 0 1044 1179
Size: 1044 135
BsaWI w'CCGG_w
Cuts at: 0 1142 1179
Size: 1142 37
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 994 1000 1024 1030 1179
Size: 994 6 24 6 149
Fragments arranged by size:
994 149 24 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 832 862 1179
Size: 832 30 317
Fragments arranged by size:
832 317 30
BseYI C'CCAG_C
Cuts at: 0 198 359 392 1179
Size: 198 161 33 787
Fragments arranged by size:
787 198 161 33
BsiEI CG_ry'CG
Cuts at: 0 731 842 1179
Size: 731 111 337
Fragments arranged by size:
731 337 111
BsiHKAI G_wGCw'C
Cuts at: 0 421 451 854 1157 1179
Size: 421 30 403 303 22
Fragments arranged by size:
421 403 303 30 22
Bsp1286I G_dGCh'C
Cuts at: 0 19 188 284 421 451 532 559 756
Size: 19 169 96 137 30 81 27 197
Cuts at: 756 805 854 1157 1179
Size: 49 49 303 22
Fragments arranged by size:
303 197 169 137 96 81 49 49
30 27 22 19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 149 167 1179
Size: 149 18 1012
Fragments arranged by size:
1012 149 18
BsrBI CCG'CTC
Cuts at: 0 1041 1179
Size: 1041 138
BsrDI GCAATG_nn'
Cuts at: 0 219 1179
Size: 219 960
BssHII G'CGCG_C
Cuts at: 0 644 1179
Size: 644 535
BssSI C'ACGA_G
Cuts at: 0 414 1179
Size: 414 765
BstYI r'GATC_y
Cuts at: 0 1145 1179
Size: 1145 34
BtgI C'CryG_G
Cuts at: 0 126 523 842 1179
Size: 126 397 319 337
Fragments arranged by size:
397 337 319 126
DraIII CAC_nnn'GTG
Cuts at: 0 416 1179
Size: 416 763
EaeI y'GGCC_r
Cuts at: 0 494 728 839 1179
Size: 494 234 111 340
Fragments arranged by size:
494 340 234 111
EagI C'GGCC_G
Cuts at: 0 728 839 1179
Size: 728 111 340
Fragments arranged by size:
728 340 111
EarI CTCTTCn'nnn_
Cuts at: 0 193 437 1179
Size: 193 244 742
Fragments arranged by size:
742 244 193
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 487 580 1179
Size: 487 93 599
Fragments arranged by size:
599 487 93
EcoICRI GAG'CTC
Cuts at: 0 449 852 1155 1179
Size: 449 403 303 24
Fragments arranged by size:
449 403 303 24
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 546 1015 1021 1141 1179
Size: 546 469 6 120 38
Fragments arranged by size:
546 469 120 38 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 598 1179
Size: 598 581
EcoO109I rG'GnC_Cy
Cuts at: 0 259 281 528 1179
Size: 259 22 247 651
Fragments arranged by size:
651 259 247 22
HaeII r_GCGC'y
Cuts at: 0 403 969 1047 1179
Size: 403 566 78 132
Fragments arranged by size:
566 403 132 78
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 208 240 1179
Size: 208 32 939
Fragments arranged by size:
939 208 32
HincII GTy'rAC
Cuts at: 0 42 1179
Size: 42 1137
KasI G'GCGC_C
Cuts at: 0 1043 1179
Size: 1043 136
MluI A'CGCG_T
Cuts at: 0 827 1179
Size: 827 352
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 937 1179
Size: 937 242
MspA1I CmG'CkG
Cuts at: 0 236 525 566 632 794 844 1179
Size: 236 289 41 66 162 50 335
Fragments arranged by size:
335 289 236 162 66 50 41
NarI GG'CG_CC
Cuts at: 0 1044 1179
Size: 1044 135
NheI G'CTAG_C
Cuts at: 0 714 1179
Size: 714 465
PasI CC'CwG_GG
Cuts at: 0 552 903 1179
Size: 552 351 276
Fragments arranged by size:
552 351 276
PflMI CCAn_nnn'nTGG
Cuts at: 0 580 993 1179
Size: 580 413 186
Fragments arranged by size:
580 413 186
PfoI T'CCnGG_A
Cuts at: 0 1148 1179
Size: 1148 31
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1000 1032 1179
Size: 1000 32 147
Fragments arranged by size:
1000 147 32
PpuMI rG'GwC_Cy
Cuts at: 0 259 1179
Size: 259 920
PspOMI G'GGCC_C
Cuts at: 0 280 528 1179
Size: 280 248 651
Fragments arranged by size:
651 280 248
PstI C_TGCA'G
Cuts at: 0 176 1179
Size: 176 1003
SacI G_AGCT'C
Cuts at: 0 451 854 1157 1179
Size: 451 403 303 22
Fragments arranged by size:
451 403 303 22
SacII CC_GC'GG
Cuts at: 0 526 845 1179
Size: 526 319 334
Fragments arranged by size:
526 334 319
SalI G'TCGA_C
Cuts at: 0 40 1179
Size: 40 1139
SapI GCTCTTCn'nnn_
Cuts at: 0 193 1179
Size: 193 986
SfcI C'TryA_G
Cuts at: 0 172 1179
Size: 172 1007
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 736 1179
Size: 736 443
SfoI GGC'GCC
Cuts at: 0 1045 1179
Size: 1045 134
SmaI CCC'GGG
Cuts at: 0 264 686 1179
Size: 264 422 493
Fragments arranged by size:
493 422 264
SmlI C'TyrA_G
Cuts at: 0 1034 1179
Size: 1034 145
StuI AGG'CCT
Cuts at: 0 604 1179
Size: 604 575
StyI C'CwwG_G
Cuts at: 0 617 1179
Size: 617 562
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 817 849 1179
Size: 817 32 330
Fragments arranged by size:
817 330 32
XhoI C'TCGA_G
Cuts at: 0 1034 1179
Size: 1034 145
XmaI C'CCGG_G
Cuts at: 0 262 684 1179
Size: 262 422 495
Fragments arranged by size:
495 422 262
Enzymes that do cut:
AccI AcuI AfeI AflIII AloI AlwNI ApaI AvaI
BamHI BanI BanII BbeI BglI Bme1580I BmtI BplI
BpmI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspMI BsrBI BsrDI BssHII BssSI BstYI BtgI
DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I HaeII Hin4I HincII KasI MluI MmeI MspA1I
NarI NheI PasI PflMI PfoI PpiI PpuMI PspOMI
PstI SacI SacII SalI SapI SfcI SfiI SfoI
SmaI SmlI StuI StyI TstI XhoI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AhdI AleI
AlfI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI
BmgBI BmrI Bpu10I BpuEI BsaI BsaAI BsaBI BsgI
BsiWI BsmI BsmBI BspEI BspHI BsrFI BsrGI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI ClaI
CspCI DraI DrdI EcoRI EcoRV FalI FseI FspI
FspAI HindIII HpaI KpnI MfeI MscI MslI NaeI
NcoI NdeI NgoMIV NotI NruI NsiI NspI PacI
PciI PmeI PmlI PshAI PsiI PspXI PsrI PvuI
PvuII RsrII SanDI SbfI ScaI SexAI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI SwaI TaqII TatI
Tth111I XbaI XcmI XmnI ZraI