Restriction map for
pF1KB8388
(Linear) (Six-base) MAPSORT of: KIBB8388.seq Check: 4518 from: 1 to: 1746
KIBB8388.orf
With 182 enzymes: SgfI *
March 3, 2008 11:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 414 1746
Size: 414 1332
AatII G_ACGT'C
Cuts at: 0 766 1746
Size: 766 980
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 616 632 748 832 1111 1331 1746
Size: 616 16 116 84 279 220 415
Fragments arranged by size:
616 415 279 220 116 84 16
AlwNI CAG_nnn'CTG
Cuts at: 0 339 810 885 1266 1746
Size: 339 471 75 381 480
Fragments arranged by size:
480 471 381 339 75
ApaI G_GGCC'C
Cuts at: 0 702 1054 1610 1746
Size: 702 352 556 136
Fragments arranged by size:
702 556 352 136
ApaLI G'TGCA_C
Cuts at: 0 1477 1654 1746
Size: 1477 177 92
Fragments arranged by size:
1477 177 92
AvaI C'yCGr_G
Cuts at: 0 646 964 1746
Size: 646 318 782
Fragments arranged by size:
782 646 318
BanI G'GyrC_C
Cuts at: 0 183 222 348 373 426 603 1269 1443
Size: 183 39 126 25 53 177 666 174
Cuts at: 1443 1746
Size: 303
Fragments arranged by size:
666 303 183 177 174 126 53 39
25
BanII G_rGCy'C
Cuts at: 0 702 896 1054 1516 1610 1647 1746
Size: 702 194 158 462 94 37 99
Fragments arranged by size:
702 462 194 158 99 94 37
BbeI G_GCGC'C
Cuts at: 0 187 352 377 1447 1746
Size: 187 165 25 1070 299
Fragments arranged by size:
1070 299 187 165 25
BbsI GAAGACnn'nnnn_
Cuts at: 0 766 1222 1746
Size: 766 456 524
Fragments arranged by size:
766 524 456
BbvCI CC'TCA_GC
Cuts at: 0 293 551 1746
Size: 293 258 1195
Fragments arranged by size:
1195 293 258
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1380 1414 1746
Size: 1380 34 332
Fragments arranged by size:
1380 332 34
BglI GCCn_nnn'nGGC
Cuts at: 0 145 417 1417 1642 1746
Size: 145 272 1000 225 104
Fragments arranged by size:
1000 272 225 145 104
BlpI GC'TnA_GC
Cuts at: 0 907 1063 1746
Size: 907 156 683
Fragments arranged by size:
907 683 156
Bme1580I G_kGCm'C
Cuts at: 0 431 702 1054 1469 1481 1610 1658 1746
Size: 431 271 352 415 12 129 48 88
Fragments arranged by size:
431 415 352 271 129 88 48 12
BmrI ACTGGGnnnn_n'
Cuts at: 0 1717 1746
Size: 1717 29
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 560 949 1262 1746
Size: 40 520 389 313 484
Fragments arranged by size:
520 484 389 313 40
Bpu10I CC'TnA_GC
Cuts at: 0 293 551 1058 1646 1746
Size: 293 258 507 588 100
Fragments arranged by size:
588 507 293 258 100
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 880 1720 1746
Size: 880 840 26
Fragments arranged by size:
880 840 26
BsaI GGTCTCn'nnnn_
Cuts at: 0 715 1746
Size: 715 1031
BsaAI yAC'GTr
Cuts at: 0 70 859 1746
Size: 70 789 887
Fragments arranged by size:
887 789 70
BsaHI Gr'CG_yC
Cuts at: 0 184 349 374 763 1444 1746
Size: 184 165 25 389 681 302
Fragments arranged by size:
681 389 302 184 165 25
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 423 453 964 994 1144 1174 1498 1528
Size: 423 30 511 30 150 30 324 30
Cuts at: 1528 1746
Size: 218
Fragments arranged by size:
511 423 324 218 150 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 88 1045 1226 1746
Size: 88 957 181 520
Fragments arranged by size:
957 520 181 88
BseYI C'CCAG_C
Cuts at: 0 332 412 563 585 1076 1111 1300 1423
Size: 332 80 151 22 491 35 189 123
Cuts at: 1423 1517 1664 1746
Size: 94 147 82
Fragments arranged by size:
491 332 189 151 147 123 94 82
80 35 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1213 1746
Size: 1213 533
BsiEI CG_ry'CG
Cuts at: 0 1598 1637 1746
Size: 1598 39 109
Fragments arranged by size:
1598 109 39
BsiHKAI G_wGCw'C
Cuts at: 0 69 896 1481 1658 1688 1746
Size: 69 827 585 177 30 58
Fragments arranged by size:
827 585 177 69 58 30
BsmBI CGTCTCn'nnnn_
Cuts at: 0 4 1746
Size: 4 1742
Bsp1286I G_dGCh'C
Cuts at: 0 69 431 702 896 1054 1469 1481 1516
Size: 69 362 271 194 158 415 12 35
Cuts at: 1516 1610 1647 1658 1688 1746
Size: 94 37 11 30 58
Fragments arranged by size:
415 362 271 194 158 94 69 58
37 35 30 12 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 414 1746
Size: 414 1332
BsrBI CCG'CTC
Cuts at: 0 84 849 1746
Size: 84 765 897
Fragments arranged by size:
897 765 84
BsrFI r'CCGG_y
Cuts at: 0 207 219 360 1245 1597 1632 1746
Size: 207 12 141 885 352 35 114
Fragments arranged by size:
885 352 207 141 114 35 12
BssSI C'ACGA_G
Cuts at: 0 901 1746
Size: 901 845
BstEII G'GTnAC_C
Cuts at: 0 279 1704 1746
Size: 279 1425 42
Fragments arranged by size:
1425 279 42
BstXI CCAn_nnnn'nTGG
Cuts at: 0 135 1746
Size: 135 1611
BtgI C'CryG_G
Cuts at: 0 323 1163 1313 1541 1637 1700 1746
Size: 323 840 150 228 96 63 46
Fragments arranged by size:
840 323 228 150 96 63 46
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1305 1383 1392 1542 1746
Size: 1305 78 9 150 204
Fragments arranged by size:
1305 204 150 78 9
BtsI GCAGTG_nn'
Cuts at: 0 1553 1746
Size: 1553 193
DraIII CAC_nnn'GTG
Cuts at: 0 1164 1746
Size: 1164 582
EaeI y'GGCC_r
Cuts at: 0 137 1595 1634 1746
Size: 137 1458 39 112
Fragments arranged by size:
1458 137 112 39
EagI C'GGCC_G
Cuts at: 0 1595 1634 1746
Size: 1595 39 112
Fragments arranged by size:
1595 112 39
EarI CTCTTCn'nnn_
Cuts at: 0 634 808 1746
Size: 634 174 938
Fragments arranged by size:
938 634 174
EcoICRI GAG'CTC
Cuts at: 0 894 1746
Size: 894 852
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 560 616 632 748 832 949 1111
Size: 40 520 56 16 116 84 117 162
Cuts at: 1111 1262 1331 1746
Size: 151 69 415
Fragments arranged by size:
520 415 162 151 117 116 84 69
56 40 16
EcoO109I rG'GnC_Cy
Cuts at: 0 590 698 1606 1607 1746
Size: 590 108 908 1 139
Fragments arranged by size:
908 590 139 108 1
FseI GG_CCGG'CC
Cuts at: 0 1601 1746
Size: 1601 145
HaeII r_GCGC'y
Cuts at: 0 187 352 377 532 793 1447 1746
Size: 187 165 25 155 261 654 299
Fragments arranged by size:
654 299 261 187 165 155 25
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 687 719 753 785 964 996 1056 1088
Size: 687 32 34 32 179 32 60 32
Cuts at: 1088 1746
Size: 658
Fragments arranged by size:
687 658 179 60 34 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1587 1746
Size: 1587 159
KasI G'GCGC_C
Cuts at: 0 183 348 373 1443 1746
Size: 183 165 25 1070 303
Fragments arranged by size:
1070 303 183 165 25
MscI TGG'CCA
Cuts at: 0 139 1746
Size: 139 1607
MslI CAynn'nnrTG
Cuts at: 0 1474 1746
Size: 1474 272
MspA1I CmG'CkG
Cuts at: 0 325 555 610 882 1521 1668 1746
Size: 325 230 55 272 639 147 78
Fragments arranged by size:
639 325 272 230 147 78 55
NaeI GCC'GGC
Cuts at: 0 221 362 1599 1634 1746
Size: 221 141 1237 35 112
Fragments arranged by size:
1237 221 141 112 35
NarI GG'CG_CC
Cuts at: 0 184 349 374 1444 1746
Size: 184 165 25 1070 302
Fragments arranged by size:
1070 302 184 165 25
NcoI C'CATG_G
Cuts at: 0 1700 1746
Size: 1700 46
NgoMIV G'CCGG_C
Cuts at: 0 219 360 1597 1632 1746
Size: 219 141 1237 35 114
Fragments arranged by size:
1237 219 141 114 35
PflMI CCAn_nnn'nTGG
Cuts at: 0 478 538 918 1164 1266 1290 1671 1746
Size: 478 60 380 246 102 24 381 75
Fragments arranged by size:
478 381 380 246 102 75 60 24
PfoI T'CCnGG_A
Cuts at: 0 35 1746
Size: 35 1711
PmlI CAC'GTG
Cuts at: 0 70 859 1746
Size: 70 789 887
Fragments arranged by size:
887 789 70
PpuMI rG'GwC_Cy
Cuts at: 0 590 1746
Size: 590 1156
PshAI GACnn'nnGTC
Cuts at: 0 1702 1746
Size: 1702 44
PspOMI G'GGCC_C
Cuts at: 0 698 1050 1606 1746
Size: 698 352 556 140
Fragments arranged by size:
698 556 352 140
PstI C_TGCA'G
Cuts at: 0 1379 1746
Size: 1379 367
PvuII CAG'CTG
Cuts at: 0 555 610 1521 1668 1746
Size: 555 55 911 147 78
Fragments arranged by size:
911 555 147 78 55
SacI G_AGCT'C
Cuts at: 0 896 1746
Size: 896 850
SacII CC_GC'GG
Cuts at: 0 326 1746
Size: 326 1420
SanDI GG'GwC_CC
Cuts at: 0 590 1746
Size: 590 1156
SfcI C'TryA_G
Cuts at: 0 1375 1746
Size: 1375 371
SfoI GGC'GCC
Cuts at: 0 185 350 375 1445 1746
Size: 185 165 25 1070 301
Fragments arranged by size:
1070 301 185 165 25
SmaI CCC'GGG
Cuts at: 0 648 1746
Size: 648 1098
SmlI C'TyrA_G
Cuts at: 0 895 1735 1746
Size: 895 840 11
Fragments arranged by size:
895 840 11
StuI AGG'CCT
Cuts at: 0 979 1746
Size: 979 767
StyI C'CwwG_G
Cuts at: 0 1700 1746
Size: 1700 46
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 489 594 1157 1746
Size: 489 105 563 589
Fragments arranged by size:
589 563 489 105
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 423 455 1144 1176 1498 1530 1746
Size: 423 32 689 32 322 32 216
Fragments arranged by size:
689 423 322 216 32 32 32
XmaI C'CCGG_G
Cuts at: 0 646 1746
Size: 646 1100
ZraI GAC'GTC
Cuts at: 0 764 1746
Size: 764 982
Enzymes that do cut:
AarI AatII AcuI AlwNI ApaI ApaLI AvaI BanI
BanII BbeI BbsI BbvCI BcgI BglI BlpI Bme1580I
BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI
BsrBI BsrFI BssSI BstEII BstXI BtgI BtgZI BtsI
DraIII EaeI EagI EarI EcoICRI Eco57MI EcoO109I FseI
HaeII Hin4I HincII KasI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV PflMI PfoI PmlI PpuMI PshAI
PspOMI PstI PvuII SacI SacII SanDI SfcI SfoI
SmaI SmlI StuI StyI TaqII TstI XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BciVI BclI BfrBI BglII BmgBI BmtI
BplI BsaBI BsaWI BsiWI BsmI BspEI BspHI BsrDI
BsrGI BssHII BstAPI BstBI BstYI BstZ17I Bsu36I ClaI
CspCI DraI DrdI EciI EcoNI EcoRI EcoRV FalI
FspI FspAI HindIII HpaI KpnI MfeI MluI MmeI
NdeI NheI NotI NruI NsiI NspI PacI PasI
PciI PmeI PpiI PsiI PspXI PsrI PvuI RsrII
SalI SapI SbfI ScaI SexAI SfiI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I
XbaI XcmI XhoI XmnI