Restriction map for
pF1KB8383
(Linear) (Six-base) MAPSORT of: KIBB8383.seq Check: 5893 from: 1 to: 1737
KIBB8383.orf
With 182 enzymes: SgfI *
March 3, 2008 11:05 ..
AflIII A'CryG_T
Cuts at: 0 260 1737
Size: 260 1477
AhdI GACnn_n'nnGTC
Cuts at: 0 1670 1737
Size: 1670 67
AleI CACnn'nnGTG
Cuts at: 0 1714 1737
Size: 1714 23
AlwNI CAG_nnn'CTG
Cuts at: 0 385 1737
Size: 385 1352
ApoI r'AATT_y
Cuts at: 0 435 843 1703 1737
Size: 435 408 860 34
Fragments arranged by size:
860 435 408 34
AseI AT'TA_AT
Cuts at: 0 596 1737
Size: 596 1141
BanII G_rGCy'C
Cuts at: 0 197 870 1119 1737
Size: 197 673 249 618
Fragments arranged by size:
673 618 249 197
BbsI GAAGACnn'nnnn_
Cuts at: 0 1669 1737
Size: 1669 68
BclI T'GATC_A
Cuts at: 0 576 1737
Size: 576 1161
BfrBI ATG'CAT
Cuts at: 0 1402 1654 1737
Size: 1402 252 83
Fragments arranged by size:
1402 252 83
BglII A'GATC_T
Cuts at: 0 700 1737
Size: 700 1037
Bme1580I G_kGCm'C
Cuts at: 0 379 1737
Size: 379 1358
BmgBI CAC'GTC
Cuts at: 0 261 1737
Size: 261 1476
BmrI ACTGGGnnnn_n'
Cuts at: 0 1553 1737
Size: 1553 184
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 704 734 1456 1486 1624 1654 1737
Size: 704 30 722 30 138 30 83
Fragments arranged by size:
722 704 138 83 30 30 30
BseYI C'CCAG_C
Cuts at: 0 1103 1737
Size: 1103 634
BsiHKAI G_wGCw'C
Cuts at: 0 870 1119 1688 1737
Size: 870 249 569 49
Fragments arranged by size:
870 569 249 49
BsmI GAATG_Cn'
Cuts at: 0 594 1737
Size: 594 1143
Bsp1286I G_dGCh'C
Cuts at: 0 197 379 870 1119 1688 1737
Size: 197 182 491 249 569 49
Fragments arranged by size:
569 491 249 197 182 49
BsrDI GCAATG_nn'
Cuts at: 0 1324 1737
Size: 1324 413
BsrGI T'GTAC_A
Cuts at: 0 1524 1737
Size: 1524 213
BstAPI GCAn_nnn'nTGC
Cuts at: 0 821 1737
Size: 821 916
BstYI r'GATC_y
Cuts at: 0 609 700 1737
Size: 609 91 1037
Fragments arranged by size:
1037 609 91
DraI TTT'AAA
Cuts at: 0 764 1737
Size: 764 973
EaeI y'GGCC_r
Cuts at: 0 630 1737
Size: 630 1107
EarI CTCTTCn'nnn_
Cuts at: 0 53 185 1737
Size: 53 132 1552
Fragments arranged by size:
1552 132 53
EcoICRI GAG'CTC
Cuts at: 0 868 1117 1737
Size: 868 249 620
Fragments arranged by size:
868 620 249
EcoRI G'AATT_C
Cuts at: 0 1703 1737
Size: 1703 34
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 380 1737
Size: 380 1357
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 92 124 704 736 1260 1292 1418 1450
Size: 92 32 580 32 524 32 126 32
Cuts at: 1450 1656 1688 1737
Size: 206 32 49
Fragments arranged by size:
580 524 206 126 92 49 32 32
32 32 32
MscI TGG'CCA
Cuts at: 0 632 1737
Size: 632 1105
MslI CAynn'nnrTG
Cuts at: 0 1560 1714 1737
Size: 1560 154 23
Fragments arranged by size:
1560 154 23
NsiI A_TGCA'T
Cuts at: 0 1404 1656 1737
Size: 1404 252 81
Fragments arranged by size:
1404 252 81
NspI r_CATG'y
Cuts at: 0 1406 1658 1737
Size: 1406 252 79
Fragments arranged by size:
1406 252 79
PflMI CCAn_nnn'nTGG
Cuts at: 0 628 1737
Size: 628 1109
PfoI T'CCnGG_A
Cuts at: 0 110 1737
Size: 110 1627
PsiI TTA'TAA
Cuts at: 0 569 1737
Size: 569 1168
SacI G_AGCT'C
Cuts at: 0 870 1119 1737
Size: 870 249 618
Fragments arranged by size:
870 618 249
SapI GCTCTTCn'nnn_
Cuts at: 0 185 1737
Size: 185 1552
SspI AAT'ATT
Cuts at: 0 447 1737
Size: 447 1290
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 248 1737
Size: 248 1489
TatI w'GTAC_w
Cuts at: 0 1032 1228 1524 1737
Size: 1032 196 296 213
Fragments arranged by size:
1032 296 213 196
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 242 1737
Size: 242 1495
XmnI GAAnn'nnTTC
Cuts at: 0 832 1162 1414 1582 1737
Size: 832 330 252 168 155
Fragments arranged by size:
832 330 252 168 155
Enzymes that do cut:
AflIII AhdI AleI AlwNI ApoI AseI BanII BbsI
BclI BfrBI BglII Bme1580I BmgBI BmrI BsaXI BseYI
BsiHKAI BsmI Bsp1286I BsrDI BsrGI BstAPI BstYI DraI
EaeI EarI EcoICRI EcoRI FalI Hin4I MscI MslI
NsiI NspI PflMI PfoI PsiI SacI SapI SspI
TaqII TatI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AlfI AloI ApaI ApaLI AscI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI
BglI BlpI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BsgI BsiEI BsiWI
BsmBI BspEI BspHI BspMI BsrBI BsrFI BssHII BssSI
BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraIII DrdI EagI EciI Eco57MI EcoNI
EcoO109I EcoRV FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI PacI
PasI PciI PmeI PmlI PpiI PpuMI PshAI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacII SalI
SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StuI
StyI SwaI TstI Tth111I XbaI XhoI XmaI ZraI