Restriction map for
pF1KB8378
(Linear) (Six-base) MAPSORT of: KIBB8378.seq Check: 1772 from: 1 to: 1674
KIBB8378.orf
With 182 enzymes: SgfI *
March 3, 2008 11:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 104 162 1578 1674
Size: 104 58 1416 96
Fragments arranged by size:
1416 104 96 58
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 288 498 1036 1674
Size: 288 210 538 638
Fragments arranged by size:
638 538 288 210
AflIII A'CryG_T
Cuts at: 0 1263 1674
Size: 1263 411
AleI CACnn'nnGTG
Cuts at: 0 1234 1674
Size: 1234 440
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 958 990 1674
Size: 958 32 684
Fragments arranged by size:
958 684 32
AlwNI CAG_nnn'CTG
Cuts at: 0 264 1446 1674
Size: 264 1182 228
Fragments arranged by size:
1182 264 228
ApaI G_GGCC'C
Cuts at: 0 296 1674
Size: 296 1378
ApaLI G'TGCA_C
Cuts at: 0 342 1674
Size: 342 1332
ApoI r'AATT_y
Cuts at: 0 730 1674
Size: 730 944
AvaI C'yCGr_G
Cuts at: 0 37 450 704 1623 1674
Size: 37 413 254 919 51
Fragments arranged by size:
919 413 254 51 37
BamHI G'GATC_C
Cuts at: 0 1097 1674
Size: 1097 577
BanI G'GyrC_C
Cuts at: 0 92 1239 1674
Size: 92 1147 435
Fragments arranged by size:
1147 435 92
BanII G_rGCy'C
Cuts at: 0 13 296 476 542 1095 1490 1532 1674
Size: 13 283 180 66 553 395 42 142
Fragments arranged by size:
553 395 283 180 142 66 42 13
BbeI G_GCGC'C
Cuts at: 0 1243 1674
Size: 1243 431
BbsI GAAGACnn'nnnn_
Cuts at: 0 219 1674
Size: 219 1455
BglI GCCn_nnn'nGGC
Cuts at: 0 1613 1651 1674
Size: 1613 38 23
Fragments arranged by size:
1613 38 23
BglII A'GATC_T
Cuts at: 0 1040 1674
Size: 1040 634
BlpI GC'TnA_GC
Cuts at: 0 82 1674
Size: 82 1592
Bme1580I G_kGCm'C
Cuts at: 0 296 346 1520 1674
Size: 296 50 1174 154
Fragments arranged by size:
1174 296 154 50
BmrI ACTGGGnnnn_n'
Cuts at: 0 777 1674
Size: 777 897
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 465 1674
Size: 465 1209
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 556 1674
Size: 556 1118
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 379 847 1516 1674
Size: 379 468 669 158
Fragments arranged by size:
669 468 379 158
BsaI GGTCTCn'nnnn_
Cuts at: 0 950 1674
Size: 950 724
BsaAI yAC'GTr
Cuts at: 0 468 1674
Size: 468 1206
BsaHI Gr'CG_yC
Cuts at: 0 1240 1463 1674
Size: 1240 223 211
Fragments arranged by size:
1240 223 211
BsaWI w'CCGG_w
Cuts at: 0 194 1674
Size: 194 1480
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 833 863 958 988 1674
Size: 833 30 95 30 686
Fragments arranged by size:
833 686 95 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 265 676 763 784 1294 1674
Size: 265 411 87 21 510 380
Fragments arranged by size:
510 411 380 265 87 21
BseYI C'CCAG_C
Cuts at: 0 130 295 1674
Size: 130 165 1379
Fragments arranged by size:
1379 165 130
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 277 964 1650 1674
Size: 190 87 687 686 24
Fragments arranged by size:
687 686 190 87 24
BsiEI CG_ry'CG
Cuts at: 0 1277 1674
Size: 1277 397
BsiHKAI G_wGCw'C
Cuts at: 0 346 476 542 1288 1532 1674
Size: 346 130 66 746 244 142
Fragments arranged by size:
746 346 244 142 130 66
BsmBI CGTCTCn'nnnn_
Cuts at: 0 813 1674
Size: 813 861
Bsp1286I G_dGCh'C
Cuts at: 0 13 296 346 476 542 1095 1288 1490
Size: 13 283 50 130 66 553 193 202
Cuts at: 1490 1520 1532 1674
Size: 30 12 142
Fragments arranged by size:
553 283 202 193 142 130 66 50
30 13 12
BspEI T'CCGG_A
Cuts at: 0 194 1674
Size: 194 1480
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 104 162 1431 1578 1674
Size: 104 58 1269 147 96
Fragments arranged by size:
1269 147 104 96 58
BsrBI CCG'CTC
Cuts at: 0 114 1674
Size: 114 1560
BsrFI r'CCGG_y
Cuts at: 0 89 1674
Size: 89 1585
BsrGI T'GTAC_A
Cuts at: 0 578 1674
Size: 578 1096
BstAPI GCAn_nnn'nTGC
Cuts at: 0 264 341 1546 1674
Size: 264 77 1205 128
Fragments arranged by size:
1205 264 128 77
BstEII G'GTnAC_C
Cuts at: 0 375 1338 1674
Size: 375 963 336
Fragments arranged by size:
963 375 336
BstXI CCAn_nnnn'nTGG
Cuts at: 0 495 1236 1674
Size: 495 741 438
Fragments arranged by size:
741 495 438
BstYI r'GATC_y
Cuts at: 0 914 1040 1097 1674
Size: 914 126 57 577
Fragments arranged by size:
914 577 126 57
Bsu36I CC'TnA_GG
Cuts at: 0 1148 1674
Size: 1148 526
BtgI C'CryG_G
Cuts at: 0 926 961 1031 1477 1674
Size: 926 35 70 446 197
Fragments arranged by size:
926 446 197 70 35
DraIII CAC_nnn'GTG
Cuts at: 0 876 1483 1674
Size: 876 607 191
Fragments arranged by size:
876 607 191
DrdI GACnn_nn'nnGTC
Cuts at: 0 1441 1462 1674
Size: 1441 21 212
Fragments arranged by size:
1441 212 21
EaeI y'GGCC_r
Cuts at: 0 929 1274 1349 1556 1571 1674
Size: 929 345 75 207 15 103
Fragments arranged by size:
929 345 207 103 75 15
EagI C'GGCC_G
Cuts at: 0 1274 1674
Size: 1274 400
EarI CTCTTCn'nnn_
Cuts at: 0 517 811 1674
Size: 517 294 863
Fragments arranged by size:
863 517 294
EcoICRI GAG'CTC
Cuts at: 0 474 540 1530 1674
Size: 474 66 990 144
Fragments arranged by size:
990 474 144 66
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 288 498 556 1036 1674
Size: 288 210 58 480 638
Fragments arranged by size:
638 480 288 210 58
EcoO109I rG'GnC_Cy
Cuts at: 0 507 1443 1578 1674
Size: 507 936 135 96
Fragments arranged by size:
936 507 135 96
EcoRI G'AATT_C
Cuts at: 0 730 1674
Size: 730 944
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 528 735 1674
Size: 528 207 939
Fragments arranged by size:
939 528 207
HaeII r_GCGC'y
Cuts at: 0 1243 1555 1674
Size: 1243 312 119
Fragments arranged by size:
1243 312 119
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 657 685 689 717 833 865 1082 1114
Size: 657 28 4 28 116 32 217 32
Cuts at: 1114 1674
Size: 560
Fragments arranged by size:
657 560 217 116 32 32 28 28
4
HincII GTy'rAC
Cuts at: 0 552 1674
Size: 552 1122
KasI G'GCGC_C
Cuts at: 0 1239 1674
Size: 1239 435
MluI A'CGCG_T
Cuts at: 0 1263 1674
Size: 1263 411
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 149 548 1674
Size: 149 399 1126
Fragments arranged by size:
1126 399 149
MscI TGG'CCA
Cuts at: 0 931 1558 1674
Size: 931 627 116
Fragments arranged by size:
931 627 116
MslI CAynn'nnrTG
Cuts at: 0 430 1234 1405 1435 1674
Size: 430 804 171 30 239
Fragments arranged by size:
804 430 239 171 30
MspA1I CmG'CkG
Cuts at: 0 86 299 308 387 573 656 963 971
Size: 86 213 9 79 186 83 307 8
Cuts at: 971 1674
Size: 703
Fragments arranged by size:
703 307 213 186 86 83 79 9
8
NaeI GCC'GGC
Cuts at: 0 91 1674
Size: 91 1583
NarI GG'CG_CC
Cuts at: 0 1240 1674
Size: 1240 434
NgoMIV G'CCGG_C
Cuts at: 0 89 1674
Size: 89 1585
NspI r_CATG'y
Cuts at: 0 1440 1674
Size: 1440 234
PasI CC'CwG_GG
Cuts at: 0 348 1208 1674
Size: 348 860 466
Fragments arranged by size:
860 466 348
PflMI CCAn_nnn'nTGG
Cuts at: 0 1179 1674
Size: 1179 495
PfoI T'CCnGG_A
Cuts at: 0 533 887 1445 1496 1523 1674
Size: 533 354 558 51 27 151
Fragments arranged by size:
558 533 354 151 51 27
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 958 990 1674
Size: 958 32 684
Fragments arranged by size:
958 684 32
PpuMI rG'GwC_Cy
Cuts at: 0 507 1443 1578 1674
Size: 507 936 135 96
Fragments arranged by size:
936 507 135 96
PsiI TTA'TAA
Cuts at: 0 830 1674
Size: 830 844
PspOMI G'GGCC_C
Cuts at: 0 292 1674
Size: 292 1382
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 448 480 790 822 1674
Size: 448 32 310 32 852
Fragments arranged by size:
852 448 310 32 32
PstI C_TGCA'G
Cuts at: 0 149 655 1674
Size: 149 506 1019
Fragments arranged by size:
1019 506 149
PvuII CAG'CTG
Cuts at: 0 86 308 573 656 971 1674
Size: 86 222 265 83 315 703
Fragments arranged by size:
703 315 265 222 86 83
SacI G_AGCT'C
Cuts at: 0 476 542 1532 1674
Size: 476 66 990 142
Fragments arranged by size:
990 476 142 66
SacII CC_GC'GG
Cuts at: 0 964 1674
Size: 964 710
ScaI AGT'ACT
Cuts at: 0 1600 1674
Size: 1600 74
SfcI C'TryA_G
Cuts at: 0 145 543 651 1602 1674
Size: 145 398 108 951 72
Fragments arranged by size:
951 398 145 108 72
SfoI GGC'GCC
Cuts at: 0 1241 1674
Size: 1241 433
SmaI CCC'GGG
Cuts at: 0 39 1674
Size: 39 1635
SmlI C'TyrA_G
Cuts at: 0 394 704 862 1531 1674
Size: 394 310 158 669 143
Fragments arranged by size:
669 394 310 158 143
StuI AGG'CCT
Cuts at: 0 625 672 763 1189 1616 1674
Size: 625 47 91 426 427 58
Fragments arranged by size:
625 427 426 91 58 47
StyI C'CwwG_G
Cuts at: 0 221 1253 1674
Size: 221 1032 421
Fragments arranged by size:
1032 421 221
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 156 1357 1624 1674
Size: 156 1201 267 50
Fragments arranged by size:
1201 267 156 50
TatI w'GTAC_w
Cuts at: 0 578 878 1598 1674
Size: 578 300 720 76
Fragments arranged by size:
720 578 300 76
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1144 1176 1674
Size: 1144 32 498
Fragments arranged by size:
1144 498 32
Tth111I GACn'n_nGTC
Cuts at: 0 1462 1674
Size: 1462 212
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1404 1566 1674
Size: 1404 162 108
Fragments arranged by size:
1404 162 108
XhoI C'TCGA_G
Cuts at: 0 704 1674
Size: 704 970
XmaI C'CCGG_G
Cuts at: 0 37 1674
Size: 37 1637
Enzymes that do cut:
AarI AcuI AflIII AleI AloI AlwNI ApaI ApaLI
ApoI AvaI BamHI BanI BanII BbeI BbsI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BsrGI
BstAPI BstEII BstXI BstYI Bsu36I BtgI DraIII DrdI
EaeI EagI EarI EcoICRI Eco57MI EcoO109I EcoRI FalI
HaeII Hin4I HincII KasI MluI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PasI PflMI PfoI
PpiI PpuMI PsiI PspOMI PsrI PstI PvuII SacI
SacII ScaI SfcI SfoI SmaI SmlI StuI StyI
TaqII TatI TstI Tth111I XcmI XhoI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AlfI AscI AseI AsiSI AvrII BaeI BbvCI BcgI
BciVI BclI BfrBI BmgBI BmtI Bpu10I BsaBI BsiWI
BsmI BspHI BsrDI BssHII BssSI BstBI BstZ17I BtgZI
BtsI ClaI CspCI DraI EciI EcoNI EcoRV FseI
FspI FspAI HindIII HpaI KpnI MfeI NcoI NdeI
NheI NotI NruI NsiI PacI PciI PmeI PmlI
PshAI PspXI PvuI RsrII SalI SanDI SapI SbfI
SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI XbaI XmnI ZraI