Restriction map for
pF1KB8374
(Linear) (Six-base) MAPSORT of: KIBB8374.seq Check: 5074 from: 1 to: 1884
KIBB8374.orf
With 182 enzymes: SgfI *
March 3, 2008 11:05 ..
AatII G_ACGT'C
Cuts at: 0 1304 1884
Size: 1304 580
AccI GT'mk_AC
Cuts at: 0 1340 1884
Size: 1340 544
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 506 707 1884
Size: 506 201 1177
Fragments arranged by size:
1177 506 201
AfeI AGC'GCT
Cuts at: 0 49 1884
Size: 49 1835
AleI CACnn'nnGTG
Cuts at: 0 412 1884
Size: 412 1472
AlwNI CAG_nnn'CTG
Cuts at: 0 1105 1884
Size: 1105 779
ApaI G_GGCC'C
Cuts at: 0 1441 1710 1884
Size: 1441 269 174
Fragments arranged by size:
1441 269 174
ApoI r'AATT_y
Cuts at: 0 634 1829 1884
Size: 634 1195 55
Fragments arranged by size:
1195 634 55
AvaI C'yCGr_G
Cuts at: 0 1032 1884
Size: 1032 852
BanII G_rGCy'C
Cuts at: 0 681 1441 1710 1884
Size: 681 760 269 174
Fragments arranged by size:
760 681 269 174
BbsI GAAGACnn'nnnn_
Cuts at: 0 384 948 1541 1884
Size: 384 564 593 343
Fragments arranged by size:
593 564 384 343
BlpI GC'TnA_GC
Cuts at: 0 26 51 1884
Size: 26 25 1833
Fragments arranged by size:
1833 26 25
Bme1580I G_kGCm'C
Cuts at: 0 1441 1710 1884
Size: 1441 269 174
Fragments arranged by size:
1441 269 174
BmrI ACTGGGnnnn_n'
Cuts at: 0 260 400 1884
Size: 260 140 1484
Fragments arranged by size:
1484 260 140
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 776 823 1061 1221 1884
Size: 331 445 47 238 160 663
Fragments arranged by size:
663 445 331 238 160 47
Bpu10I CC'TnA_GC
Cuts at: 0 848 1884
Size: 848 1036
BsaI GGTCTCn'nnnn_
Cuts at: 0 448 1884
Size: 448 1436
BsaBI GATnn'nnATC
Cuts at: 0 155 1884
Size: 155 1729
BsaHI Gr'CG_yC
Cuts at: 0 1301 1884
Size: 1301 583
BsaWI w'CCGG_w
Cuts at: 0 207 653 1276 1884
Size: 207 446 623 608
Fragments arranged by size:
623 608 446 207
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 447 477 1231 1261 1884
Size: 447 30 754 30 623
Fragments arranged by size:
754 623 447 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 103 234 455 1870 1884
Size: 103 131 221 1415 14
Fragments arranged by size:
1415 221 131 103 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1091 1102 1884
Size: 1091 11 782
Fragments arranged by size:
1091 782 11
BsiEI CG_ry'CG
Cuts at: 0 720 1884
Size: 720 1164
BsiHKAI G_wGCw'C
Cuts at: 0 262 347 1884
Size: 262 85 1537
Fragments arranged by size:
1537 262 85
BsmI GAATG_Cn'
Cuts at: 0 1158 1884
Size: 1158 726
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1292 1884
Size: 1292 592
Bsp1286I G_dGCh'C
Cuts at: 0 262 347 681 1441 1710 1884
Size: 262 85 334 760 269 174
Fragments arranged by size:
760 334 269 262 174 85
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1360 1884
Size: 1360 524
BsrBI CCG'CTC
Cuts at: 0 1521 1884
Size: 1521 363
BsrGI T'GTAC_A
Cuts at: 0 1247 1884
Size: 1247 637
BstEII G'GTnAC_C
Cuts at: 0 405 1393 1884
Size: 405 988 491
Fragments arranged by size:
988 491 405
BstYI r'GATC_y
Cuts at: 0 617 1884
Size: 617 1267
BtsI GCAGTG_nn'
Cuts at: 0 328 1329 1439 1884
Size: 328 1001 110 445
Fragments arranged by size:
1001 445 328 110
DrdI GACnn_nn'nnGTC
Cuts at: 0 1486 1884
Size: 1486 398
EaeI y'GGCC_r
Cuts at: 0 528 717 1667 1884
Size: 528 189 950 217
Fragments arranged by size:
950 528 217 189
EagI C'GGCC_G
Cuts at: 0 717 1884
Size: 717 1167
EarI CTCTTCn'nnn_
Cuts at: 0 62 80 482 1884
Size: 62 18 402 1402
Fragments arranged by size:
1402 402 62 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1674 1884
Size: 1674 210
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 506 707 776 823 1061 1221 1884
Size: 331 175 201 69 47 238 160 663
Fragments arranged by size:
663 331 238 201 175 160 69 47
EcoO109I rG'GnC_Cy
Cuts at: 0 314 1145 1817 1884
Size: 314 831 672 67
Fragments arranged by size:
831 672 314 67
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 66 1884
Size: 66 1818
HaeII r_GCGC'y
Cuts at: 0 51 1617 1884
Size: 51 1566 267
Fragments arranged by size:
1566 267 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 447 479 1201 1233 1666 1698 1884
Size: 447 32 722 32 433 32 186
Fragments arranged by size:
722 447 433 186 32 32 32
HincII GTy'rAC
Cuts at: 0 596 1884
Size: 596 1288
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 719 1678 1749 1884
Size: 719 959 71 135
Fragments arranged by size:
959 719 135 71
MscI TGG'CCA
Cuts at: 0 530 1669 1884
Size: 530 1139 215
Fragments arranged by size:
1139 530 215
MslI CAynn'nnrTG
Cuts at: 0 412 823 1432 1793 1884
Size: 412 411 609 361 91
Fragments arranged by size:
609 412 411 361 91
MspA1I CmG'CkG
Cuts at: 0 351 716 1086 1102 1884
Size: 351 365 370 16 782
Fragments arranged by size:
782 370 365 351 16
NspI r_CATG'y
Cuts at: 0 1215 1884
Size: 1215 669
PfoI T'CCnGG_A
Cuts at: 0 1198 1884
Size: 1198 686
PpuMI rG'GwC_Cy
Cuts at: 0 314 1145 1817 1884
Size: 314 831 672 67
Fragments arranged by size:
831 672 314 67
PspOMI G'GGCC_C
Cuts at: 0 1437 1706 1884
Size: 1437 269 178
Fragments arranged by size:
1437 269 178
PstI C_TGCA'G
Cuts at: 0 323 356 1884
Size: 323 33 1528
Fragments arranged by size:
1528 323 33
PvuII CAG'CTG
Cuts at: 0 351 1102 1884
Size: 351 751 782
Fragments arranged by size:
782 751 351
SanDI GG'GwC_CC
Cuts at: 0 1145 1884
Size: 1145 739
SfcI C'TryA_G
Cuts at: 0 319 352 711 1884
Size: 319 33 359 1173
Fragments arranged by size:
1173 359 319 33
StyI C'CwwG_G
Cuts at: 0 427 911 1398 1884
Size: 427 484 487 486
Fragments arranged by size:
487 486 484 427
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 424 972 1884
Size: 424 548 912
Fragments arranged by size:
912 548 424
TatI w'GTAC_w
Cuts at: 0 1124 1247 1884
Size: 1124 123 637
Fragments arranged by size:
1124 637 123
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 952 984 1635 1667 1884
Size: 952 32 651 32 217
Fragments arranged by size:
952 651 217 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 810 898 1821 1884
Size: 810 88 923 63
Fragments arranged by size:
923 810 88 63
XmnI GAAnn'nnTTC
Cuts at: 0 134 1884
Size: 134 1750
ZraI GAC'GTC
Cuts at: 0 1302 1884
Size: 1302 582
Enzymes that do cut:
AatII AccI AcuI AfeI AleI AlwNI ApaI ApoI
AvaI BanII BbsI BlpI Bme1580I BmrI BpmI Bpu10I
BsaI BsaBI BsaHI BsaWI BsaXI BseRI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrGI BstEII
BstYI BtsI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I FalI HaeII Hin4I HincII MmeI MscI MslI
MspA1I NspI PfoI PpuMI PspOMI PstI PvuII SanDI
SfcI StyI TaqII TatI TstI XcmI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AflII AflIII AgeI AhdI AlfI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BanI BbeI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BmgBI BmtI BplI BpuEI BsaAI BseYI BsiWI
BspEI BspHI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI
DraIII EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI
HindIII HpaI KasI KpnI MfeI MluI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
Tth111I XbaI XhoI XmaI