(Linear) (Six-base) MAPSORT of: KIBB8370.seq  Check: 5372  from: 1  to: 483



KIBB8370.orf

 With 182 enzymes: SgfI * 

                        July 2, 2008 13:23  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     469     483 
   Size:        469      14
 
ApoI r'AATT_y

Cuts at:      0      61     483 
   Size:         61     422
 
AvaI C'yCGr_G

Cuts at:      0      14     124     483 
   Size:         14     110     359

  Fragments arranged by size: 

                 359     110      14
 
BanI G'GyrC_C

Cuts at:      0     312     483 
   Size:        312     171
 
BlpI GC'TnA_GC

Cuts at:      0     301     483 
   Size:        301     182
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     261     483 
   Size:        261     222
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      46     205     483 
   Size:         46     159     278

  Fragments arranged by size: 

                 278     159      46
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     129     483 
   Size:        129     354
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     310     336     339     342     345     348     357     454 
   Size:        310      26       3       3       3       3       9      97

Cuts at:    454     483 
   Size:         29

  Fragments arranged by size: 

                 310      97      29      26       9       3       3       3
                   3
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     386     483 
   Size:        386      97
 
BsrBI CCG'CTC

Cuts at:      0      54     483 
   Size:         54     429
 
BsrFI r'CCGG_y

Cuts at:      0     309     483 
   Size:        309     174
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     274     483 
   Size:        274     209
 
BstYI r'GATC_y

Cuts at:      0     249     483 
   Size:        249     234
 
BtgI C'CryG_G

Cuts at:      0     161     483 
   Size:        161     322
 
EarI CTCTTCn'nnn_

Cuts at:      0     431     483 
   Size:        431      52
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      31     483 
   Size:         31     452
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      46     205     483 
   Size:         46     159     278

  Fragments arranged by size: 

                 278     159      46
 
EcoO109I rG'GnC_Cy

Cuts at:      0     213     341     483 
   Size:        213     128     142

  Fragments arranged by size: 

                 213     142     128
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     214     483 
   Size:        214     269
 
HaeII r_GCGC'y

Cuts at:      0      33     483 
   Size:         33     450
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     386     410     483 
   Size:        386      24      73

  Fragments arranged by size: 

                 386      73      24
 
MslI CAynn'nnrTG

Cuts at:      0     166     483 
   Size:        166     317
 
NcoI C'CATG_G

Cuts at:      0     161     483 
   Size:        161     322
 
PpuMI rG'GwC_Cy

Cuts at:      0     213     341     483 
   Size:        213     128     142

  Fragments arranged by size: 

                 213     142     128
 
PstI C_TGCA'G

Cuts at:      0     481     483 
   Size:        481       2
 
SanDI GG'GwC_CC

Cuts at:      0     213     483 
   Size:        213     270
 
SfcI C'TryA_G

Cuts at:      0     477     483 
   Size:        477       6
 
SmlI C'TyrA_G

Cuts at:      0     124     483 
   Size:        124     359
 
StyI C'CwwG_G

Cuts at:      0     161     207     419     483 
   Size:        161      46     212      64

  Fragments arranged by size: 

                 212     161      64      46
 
XhoI C'TCGA_G

Cuts at:      0     124     483 
   Size:        124     359
 

 Enzymes that do cut:

    AlwNI     ApoI     AvaI     BanI     BlpI     BmrI     BpmI     BsaI
    BseRI    BspMI    BsrBI    BsrFI    BstXI    BstYI     BtgI     EarI
     EciI  Eco57MI EcoO109I     FalI    HaeII     MmeI     MslI     NcoI
    PpuMI     PstI    SanDI     SfcI     SmlI     StyI     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
 Bme1580I    BmgBI     BmtI     BplI   Bpu10I    BpuEI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BsrDI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI  EcoICRI
    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI   MspA1I
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XmaI     XmnI     ZraI