Restriction map for
pF1KB8369
(Linear) (Six-base) MAPSORT of: KIBB8369.seq Check: 5867 from: 1 to: 594
KIBB8369.orf
With 182 enzymes: SgfI *
March 3, 2008 11:05 ..
AatII G_ACGT'C
Cuts at: 0 588 594
Size: 588 6
ApoI r'AATT_y
Cuts at: 0 180 594
Size: 180 414
AvaI C'yCGr_G
Cuts at: 0 125 253 594
Size: 125 128 341
Fragments arranged by size:
341 128 125
BanI G'GyrC_C
Cuts at: 0 289 300 345 594
Size: 289 11 45 249
Fragments arranged by size:
289 249 45 11
BanII G_rGCy'C
Cuts at: 0 31 363 594
Size: 31 332 231
Fragments arranged by size:
332 231 31
BbeI G_GCGC'C
Cuts at: 0 349 594
Size: 349 245
BbsI GAAGACnn'nnnn_
Cuts at: 0 588 594
Size: 588 6
BglI GCCn_nnn'nGGC
Cuts at: 0 58 323 594
Size: 58 265 271
Fragments arranged by size:
271 265 58
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 594
Size: 58 536
BsaHI Gr'CG_yC
Cuts at: 0 50 346 559 585 594
Size: 50 296 213 26 9
Fragments arranged by size:
296 213 50 26 9
BsiHKAI G_wGCw'C
Cuts at: 0 68 594
Size: 68 526
Bsp1286I G_dGCh'C
Cuts at: 0 31 68 363 594
Size: 31 37 295 231
Fragments arranged by size:
295 231 37 31
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 471 594
Size: 471 123
BsrBI CCG'CTC
Cuts at: 0 42 594
Size: 42 552
BsrDI GCAATG_nn'
Cuts at: 0 445 594
Size: 445 149
BsrFI r'CCGG_y
Cuts at: 0 303 541 594
Size: 303 238 53
Fragments arranged by size:
303 238 53
BtgI C'CryG_G
Cuts at: 0 274 594
Size: 274 320
BtsI GCAGTG_nn'
Cuts at: 0 141 594
Size: 141 453
DrdI GACnn_nn'nnGTC
Cuts at: 0 408 594
Size: 408 186
EarI CTCTTCn'nnn_
Cuts at: 0 100 110 152 594
Size: 100 10 42 442
Fragments arranged by size:
442 100 42 10
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 76 594
Size: 76 518
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 594
Size: 58 536
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 264 594
Size: 264 330
FspI TGC'GCA
Cuts at: 0 444 594
Size: 444 150
HaeII r_GCGC'y
Cuts at: 0 349 594
Size: 349 245
HincII GTy'rAC
Cuts at: 0 122 594
Size: 122 472
HpaI GTT'AAC
Cuts at: 0 122 594
Size: 122 472
KasI G'GCGC_C
Cuts at: 0 345 594
Size: 345 249
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 392 594
Size: 392 202
MspA1I CmG'CkG
Cuts at: 0 276 329 594
Size: 276 53 265
Fragments arranged by size:
276 265 53
NaeI GCC'GGC
Cuts at: 0 305 594
Size: 305 289
NarI GG'CG_CC
Cuts at: 0 346 594
Size: 346 248
NgoMIV G'CCGG_C
Cuts at: 0 303 594
Size: 303 291
PstI C_TGCA'G
Cuts at: 0 88 148 594
Size: 88 60 446
Fragments arranged by size:
446 88 60
SacII CC_GC'GG
Cuts at: 0 277 594
Size: 277 317
SbfI CC_TGCA'GG
Cuts at: 0 88 594
Size: 88 506
SfcI C'TryA_G
Cuts at: 0 84 144 264 594
Size: 84 60 120 330
Fragments arranged by size:
330 120 84 60
SfoI GGC'GCC
Cuts at: 0 347 594
Size: 347 247
SmaI CCC'GGG
Cuts at: 0 127 594
Size: 127 467
XmaI C'CCGG_G
Cuts at: 0 125 594
Size: 125 469
XmnI GAAnn'nnTTC
Cuts at: 0 94 594
Size: 94 500
ZraI GAC'GTC
Cuts at: 0 586 594
Size: 586 8
Enzymes that do cut:
AatII ApoI AvaI BanI BanII BbeI BbsI BglI
BpmI BsaHI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI
BtgI BtsI DrdI EarI EciI Eco57MI FalI FspI
HaeII HincII HpaI KasI MmeI MspA1I NaeI NarI
NgoMIV PstI SacII SbfI SfcI SfoI SmaI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII EaeI EagI EcoICRI EcoNI EcoO109I EcoRI EcoRV
FseI FspAI Hin4I HindIII KpnI MfeI MluI MscI
MslI NcoI NdeI NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacI SalI SanDI SapI ScaI SexAI SfiI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI TaqII TatI TstI Tth111I XbaI
XcmI XhoI