(Linear) (Six-base) MAPSORT of: KIBB8367.seq  Check: 5307  from: 1  to: 894



KIBB8367.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:05  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     659     894 
   Size:        659     235
 
AccI GT'mk_AC

Cuts at:      0     218     894 
   Size:        218     676
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     538     894 
   Size:        538     356
 
AlwNI CAG_nnn'CTG

Cuts at:      0     490     894 
   Size:        490     404
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     169     894 
   Size:        169     725
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     363     894 
   Size:        363     531
 
Bme1580I G_kGCm'C

Cuts at:      0     339     894 
   Size:        339     555
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     205     894 
   Size:        205     689
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     159     383     647     894 
   Size:        159     224     264     247

  Fragments arranged by size: 

                 264     247     224     159
 
BsaWI w'CCGG_w

Cuts at:      0     281     894 
   Size:        281     613
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      16      21      24     894 
   Size:         16       5       3     870

  Fragments arranged by size: 

                 870      16       5       3
 
BsmI GAATG_Cn'

Cuts at:      0     692     894 
   Size:        692     202
 
Bsp1286I G_dGCh'C

Cuts at:      0     339     894 
   Size:        339     555
 
BspEI T'CCGG_A

Cuts at:      0     281     894 
   Size:        281     613
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     659     894 
   Size:        659     235
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     693     728     894 
   Size:        693      35     166

  Fragments arranged by size: 

                 693     166      35
 
DraI TTT'AAA

Cuts at:      0     249     894 
   Size:        249     645
 
EarI CTCTTCn'nnn_

Cuts at:      0     239     359     432     817     894 
   Size:        239     120      73     385      77

  Fragments arranged by size: 

                 385     239     120      77      73
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     159     383     647     894 
   Size:        159     224     264     247

  Fragments arranged by size: 

                 264     247     224     159
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     364     894 
   Size:        364     530
 
EcoRV GAT'ATC

Cuts at:      0     664     894 
   Size:        664     230
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     181     639     894 
   Size:        181     458     255

  Fragments arranged by size: 

                 458     255     181
 
MslI CAynn'nnrTG

Cuts at:      0     850     894 
   Size:        850      44
 
PasI CC'CwG_GG

Cuts at:      0      60     894 
   Size:         60     834
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     360     894 
   Size:        360     534
 
PsiI TTA'TAA

Cuts at:      0     889     894 
   Size:        889       5
 
SexAI A'CCwGG_T

Cuts at:      0      47     894 
   Size:         47     847
 
SspI AAT'ATT

Cuts at:      0     634     894 
   Size:        634     260
 
StuI AGG'CCT

Cuts at:      0      76     894 
   Size:         76     818
 
StyI C'CwwG_G

Cuts at:      0     765     894 
   Size:        765     129
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     865     894 
   Size:        865      29
 

 Enzymes that do cut:

     AarI     AccI     AlfI    AlwNI     BbsI    BciVI Bme1580I     BmrI
     BpmI    BsaWI    BseRI     BsmI Bsp1286I    BspEI    BspMI    CspCI
     DraI     EarI  Eco57MI    EcoNI    EcoRV     MmeI     MslI     PasI
    PflMI     PsiI    SexAI     SspI     StuI     StyI     TstI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI     BcgI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspHI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI EcoO109I
    EcoRI     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PciI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SwaI    TaqII     TatI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI