(Linear) (Six-base) MAPSORT of: KIBB8366.seq  Check: 5488  from: 1  to: 534



KIBB8366.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:05  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     357     534 
   Size:        357     177
 
ApoI r'AATT_y

Cuts at:      0      99     534 
   Size:         99     435
 
BanI G'GyrC_C

Cuts at:      0      25     203     534 
   Size:         25     178     331

  Fragments arranged by size: 

                 331     178      25
 
BbeI G_GCGC'C

Cuts at:      0      29     534 
   Size:         29     505
 
Bme1580I G_kGCm'C

Cuts at:      0     208     534 
   Size:        208     326
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     134     534 
   Size:        134     400
 
BsaHI Gr'CG_yC

Cuts at:      0      26     268     534 
   Size:         26     242     266

  Fragments arranged by size: 

                 266     242      26
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     121     353     534 
   Size:        121     232     181

  Fragments arranged by size: 

                 232     181     121
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      39     179     399     534 
   Size:         39     140     220     135

  Fragments arranged by size: 

                 220     140     135      39
 
Bsp1286I G_dGCh'C

Cuts at:      0     208     534 
   Size:        208     326
 
Bsu36I CC'TnA_GG

Cuts at:      0     146     534 
   Size:        146     388
 
BtsI GCAGTG_nn'

Cuts at:      0      40     534 
   Size:         40     494
 
DraIII CAC_nnn'GTG

Cuts at:      0      84     534 
   Size:         84     450
 
EaeI y'GGCC_r

Cuts at:      0     257     534 
   Size:        257     277
 
EarI CTCTTCn'nnn_

Cuts at:      0     437     512     534 
   Size:        437      75      22

  Fragments arranged by size: 

                 437      75      22
 
EcoRI G'AATT_C

Cuts at:      0      99     534 
   Size:         99     435
 
HaeII r_GCGC'y

Cuts at:      0      29     534 
   Size:         29     505
 
HincII GTy'rAC

Cuts at:      0     321     534 
   Size:        321     213
 
KasI G'GCGC_C

Cuts at:      0      25     534 
   Size:         25     509
 
MspA1I CmG'CkG

Cuts at:      0      23     534 
   Size:         23     511
 
NarI GG'CG_CC

Cuts at:      0      26     534 
   Size:         26     508
 
NspI r_CATG'y

Cuts at:      0     422     534 
   Size:        422     112
 
PasI CC'CwG_GG

Cuts at:      0      60     534 
   Size:         60     474
 
SfoI GGC'GCC

Cuts at:      0      27     534 
   Size:         27     507
 
SspI AAT'ATT

Cuts at:      0     528     534 
   Size:        528       6
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      96     128     534 
   Size:         96      32     406

  Fragments arranged by size: 

                 406      96      32
 

 Enzymes that do cut:

    AlwNI     ApoI     BanI     BbeI Bme1580I     BsaI    BsaHI    BseRI
     BsgI Bsp1286I   Bsu36I     BtsI   DraIII     EaeI     EarI    EcoRI
    HaeII   HincII     KasI   MspA1I     NarI     NspI     PasI     SfoI
     SspI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
    BsaAI    BsaBI    BsaWI    BsaXI    BseYI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I     BtgI    BtgZI     ClaI    CspCI     DraI     DrdI     EagI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV     FalI     FseI
     FspI    FspAI    Hin4I  HindIII     HpaI     KpnI     MfeI     MluI
     MmeI     MscI     MslI     NaeI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI     StyI
     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI