Restriction map for
pF1KB8363
(Linear) (Six-base) MAPSORT of: KIBB8363.seq Check: 4402 from: 1 to: 1509
KIBB8363.orf
With 182 enzymes: SgfI *
March 3, 2008 11:05 ..
Acc65I G'GTAC_C
Cuts at: 0 1094 1509
Size: 1094 415
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 1509
Size: 74 1435
AflIII A'CryG_T
Cuts at: 0 557 1509
Size: 557 952
AhdI GACnn_n'nnGTC
Cuts at: 0 930 1509
Size: 930 579
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 394 1509
Size: 394 1115
AlwNI CAG_nnn'CTG
Cuts at: 0 148 218 922 1509
Size: 148 70 704 587
Fragments arranged by size:
704 587 148 70
ApaI G_GGCC'C
Cuts at: 0 1301 1509
Size: 1301 208
ApoI r'AATT_y
Cuts at: 0 247 1234 1248 1509
Size: 247 987 14 261
Fragments arranged by size:
987 261 247 14
AvaI C'yCGr_G
Cuts at: 0 1210 1509
Size: 1210 299
BanI G'GyrC_C
Cuts at: 0 1094 1509
Size: 1094 415
BanII G_rGCy'C
Cuts at: 0 419 1301 1509
Size: 419 882 208
Fragments arranged by size:
882 419 208
BbsI GAAGACnn'nnnn_
Cuts at: 0 1387 1509
Size: 1387 122
BfrBI ATG'CAT
Cuts at: 0 381 667 1509
Size: 381 286 842
Fragments arranged by size:
842 381 286
Bme1580I G_kGCm'C
Cuts at: 0 130 1301 1509
Size: 130 1171 208
Fragments arranged by size:
1171 208 130
BmgBI CAC'GTC
Cuts at: 0 558 1509
Size: 558 951
BmrI ACTGGGnnnn_n'
Cuts at: 0 1153 1509
Size: 1153 356
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 290 1509
Size: 290 1219
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 285 630 1509
Size: 285 345 879
Fragments arranged by size:
879 345 285
BsaAI yAC'GTr
Cuts at: 0 1377 1509
Size: 1377 132
BsaBI GATnn'nnATC
Cuts at: 0 205 1509
Size: 205 1304
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 79 541 670 1509
Size: 79 462 129 839
Fragments arranged by size:
839 462 129 79
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1331 1509
Size: 1331 178
BsiHKAI G_wGCw'C
Cuts at: 0 419 1509
Size: 419 1090
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1359 1509
Size: 1359 150
Bsp1286I G_dGCh'C
Cuts at: 0 130 419 1301 1509
Size: 130 289 882 208
Fragments arranged by size:
882 289 208 130
BsrDI GCAATG_nn'
Cuts at: 0 962 1384 1509
Size: 962 422 125
Fragments arranged by size:
962 422 125
BstAPI GCAn_nnn'nTGC
Cuts at: 0 218 1509
Size: 218 1291
BstEII G'GTnAC_C
Cuts at: 0 531 865 1509
Size: 531 334 644
Fragments arranged by size:
644 531 334
BstXI CCAn_nnnn'nTGG
Cuts at: 0 419 1509
Size: 419 1090
BstYI r'GATC_y
Cuts at: 0 263 614 1509
Size: 263 351 895
Fragments arranged by size:
895 351 263
BtgI C'CryG_G
Cuts at: 0 183 1302 1328 1509
Size: 183 1119 26 181
Fragments arranged by size:
1119 183 181 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1308 1509
Size: 1308 201
BtsI GCAGTG_nn'
Cuts at: 0 219 373 1509
Size: 219 154 1136
Fragments arranged by size:
1136 219 154
ClaI AT'CG_AT
Cuts at: 0 1148 1509
Size: 1148 361
EarI CTCTTCn'nnn_
Cuts at: 0 610 800 980 1509
Size: 610 190 180 529
Fragments arranged by size:
610 529 190 180
EcoICRI GAG'CTC
Cuts at: 0 417 1509
Size: 417 1092
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 290 1509
Size: 74 216 1219
Fragments arranged by size:
1219 216 74
EcoO109I rG'GnC_Cy
Cuts at: 0 145 460 1298 1509
Size: 145 315 838 211
Fragments arranged by size:
838 315 211 145
EcoRI G'AATT_C
Cuts at: 0 247 1509
Size: 247 1262
FspI TGC'GCA
Cuts at: 0 1123 1509
Size: 1123 386
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 115 147 161 193 248 280 903 916
Size: 115 32 14 32 55 32 623 13
Cuts at: 916 935 948 1509
Size: 19 13 561
Fragments arranged by size:
623 561 115 55 32 32 32 19
14 13 13
HincII GTy'rAC
Cuts at: 0 563 1509
Size: 563 946
KpnI G_GTAC'C
Cuts at: 0 1098 1509
Size: 1098 411
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 503 1509
Size: 503 1006
MslI CAynn'nnrTG
Cuts at: 0 376 977 1327 1333 1442 1509
Size: 376 601 350 6 109 67
Fragments arranged by size:
601 376 350 109 67 6
NcoI C'CATG_G
Cuts at: 0 1328 1509
Size: 1328 181
NsiI A_TGCA'T
Cuts at: 0 383 669 1509
Size: 383 286 840
Fragments arranged by size:
840 383 286
NspI r_CATG'y
Cuts at: 0 1092 1461 1509
Size: 1092 369 48
Fragments arranged by size:
1092 369 48
PflMI CCAn_nnn'nTGG
Cuts at: 0 148 1509
Size: 148 1361
PmlI CAC'GTG
Cuts at: 0 1377 1509
Size: 1377 132
PpuMI rG'GwC_Cy
Cuts at: 0 145 1509
Size: 145 1364
PshAI GACnn'nnGTC
Cuts at: 0 1496 1509
Size: 1496 13
PsiI TTA'TAA
Cuts at: 0 32 1509
Size: 32 1477
PspOMI G'GGCC_C
Cuts at: 0 1297 1509
Size: 1297 212
PspXI vC'TCGA_Gb
Cuts at: 0 1210 1509
Size: 1210 299
PstI C_TGCA'G
Cuts at: 0 368 1509
Size: 368 1141
SacI G_AGCT'C
Cuts at: 0 419 1509
Size: 419 1090
SapI GCTCTTCn'nnn_
Cuts at: 0 610 1509
Size: 610 899
SexAI A'CCwGG_T
Cuts at: 0 141 1509
Size: 141 1368
SfcI C'TryA_G
Cuts at: 0 364 1509
Size: 364 1145
SmlI C'TyrA_G
Cuts at: 0 300 609 1210 1509
Size: 300 309 601 299
Fragments arranged by size:
601 309 300 299
SphI G_CATG'C
Cuts at: 0 1092 1509
Size: 1092 417
SspI AAT'ATT
Cuts at: 0 1191 1509
Size: 1191 318
StyI C'CwwG_G
Cuts at: 0 651 1328 1509
Size: 651 677 181
Fragments arranged by size:
677 651 181
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1454 1509
Size: 1454 55
TatI w'GTAC_w
Cuts at: 0 1319 1509
Size: 1319 190
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 198 230 1414 1446 1509
Size: 198 32 1184 32 63
Fragments arranged by size:
1184 198 63 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1114 1509
Size: 1114 395
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1015 1509
Size: 1015 494
XhoI C'TCGA_G
Cuts at: 0 1210 1509
Size: 1210 299
Enzymes that do cut:
Acc65I AcuI AflIII AhdI AlfI AlwNI ApaI ApoI
AvaI BanI BanII BbsI BfrBI Bme1580I BmgBI BmrI
BpmI BpuEI BsaAI BsaBI BseRI BsgI BsiHKAI BsmBI
Bsp1286I BsrDI BstAPI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI EarI EcoICRI Eco57MI EcoO109I EcoRI FspI
Hin4I HincII KpnI MmeI MslI NcoI NsiI NspI
PflMI PmlI PpuMI PshAI PsiI PspOMI PspXI PstI
SacI SapI SexAI SfcI SmlI SphI SspI StyI
TaqII TatI TstI Tth111I XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AleI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbvCI BcgI BciVI BclI BglI BglII BlpI
BmtI BplI Bpu10I BsaI BsaHI BsaWI BsaXI BseYI
BsiEI BsiWI BsmI BspEI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstBI BstZ17I Bsu36I CspCI DraI
DraIII DrdI EaeI EagI EciI EcoNI EcoRV FalI
FseI FspAI HaeII HindIII HpaI KasI MfeI MluI
MscI MspA1I NaeI NarI NdeI NgoMIV NheI NotI
NruI PacI PasI PciI PfoI PmeI PpiI PsrI
PvuI PvuII RsrII SacII SalI SanDI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
StuI SwaI XbaI XmaI XmnI ZraI