(Linear) (Six-base) MAPSORT of: KIBB8360.seq  Check: 2670  from: 1  to: 504



KIBB8360.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:05  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     110     364     385     504 
   Size:        110     254      21     119

  Fragments arranged by size: 

                 254     119     110      21
 
ApoI r'AATT_y

Cuts at:      0      34     504 
   Size:         34     470
 
BanII G_rGCy'C

Cuts at:      0     375     504 
   Size:        375     129
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     247     352     403     504 
   Size:        247     105      51     101

  Fragments arranged by size: 

                 247     105     101      51
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      10      44     504 
   Size:         10      34     460

  Fragments arranged by size: 

                 460      34      10
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      75     504 
   Size:         75     429
 
BglI GCCn_nnn'nGGC

Cuts at:      0      52     504 
   Size:         52     452
 
Bme1580I G_kGCm'C

Cuts at:      0     170     196     208     504 
   Size:        170      26      12     296

  Fragments arranged by size: 

                 296     170      26      12
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     236     504 
   Size:        236     268
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     431     504 
   Size:        431      73
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     147     177     504 
   Size:        147      30     327

  Fragments arranged by size: 

                 327     147      30
 
BseYI C'CCAG_C

Cuts at:      0     189     504 
   Size:        189     315
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     361     504 
   Size:        361     143
 
BsmI GAATG_Cn'

Cuts at:      0      55     394     504 
   Size:         55     339     110

  Fragments arranged by size: 

                 339     110      55
 
Bsp1286I G_dGCh'C

Cuts at:      0     170     196     208     375     504 
   Size:        170      26      12     167     129

  Fragments arranged by size: 

                 170     167     129      26      12
 
BstYI r'GATC_y

Cuts at:      0     257     504 
   Size:        257     247
 
BtsI GCAGTG_nn'

Cuts at:      0     157     504 
   Size:        157     347
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     402     437     504 
   Size:        402      35      67

  Fragments arranged by size: 

                 402      67      35
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     110     236     364     385     504 
   Size:        110     126     128      21     119

  Fragments arranged by size: 

                 128     126     119     110      21
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     242     274     504 
   Size:        242      32     230

  Fragments arranged by size: 

                 242     230      32
 
MspA1I CmG'CkG

Cuts at:      0      43      76     504 
   Size:         43      33     428

  Fragments arranged by size: 

                 428      43      33
 
PvuII CAG'CTG

Cuts at:      0      43      76     504 
   Size:         43      33     428

  Fragments arranged by size: 

                 428      43      33
 
SmlI C'TyrA_G

Cuts at:      0     446     504 
   Size:        446      58
 
StuI AGG'CCT

Cuts at:      0     313     504 
   Size:        313     191
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     147     179     504 
   Size:        147      32     325

  Fragments arranged by size: 

                 325     147      32
 

 Enzymes that do cut:

     AcuI     ApoI    BanII     BbsI     BcgI    BciVI     BglI Bme1580I
     BpmI    BpuEI    BsaXI    BseYI     BsgI     BsmI Bsp1286I    BstYI
     BtsI    CspCI  Eco57MI    Hin4I   MspA1I    PvuII     SmlI     StuI
     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
     BbeI    BbvCI     BclI    BfrBI    BglII     BlpI    BmgBI     BmrI
     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseRI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StyI     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI