(Linear) (Six-base) MAPSORT of: KIBB8359.seq  Check: 3777  from: 1  to: 357



KIBB8359.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:05  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     270     357 
   Size:        270      87
 
AvaI C'yCGr_G

Cuts at:      0     240     357 
   Size:        240     117
 
BanII G_rGCy'C

Cuts at:      0     316     357 
   Size:        316      41
 
BglI GCCn_nnn'nGGC

Cuts at:      0     334     357 
   Size:        334      23
 
Bme1580I G_kGCm'C

Cuts at:      0     134     357 
   Size:        134     223
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     280     357 
   Size:        280      77
 
BsaWI w'CCGG_w

Cuts at:      0     289     357 
   Size:        289      68
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     107     137     357 
   Size:        107      30     220

  Fragments arranged by size: 

                 220     107      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     131     357 
   Size:        131     226
 
BsiHKAI G_wGCw'C

Cuts at:      0     143     357 
   Size:        143     214
 
Bsp1286I G_dGCh'C

Cuts at:      0     134     143     316     357 
   Size:        134       9     173      41

  Fragments arranged by size: 

                 173     134      41       9
 
BspEI T'CCGG_A

Cuts at:      0     289     357 
   Size:        289      68
 
BsrDI GCAATG_nn'

Cuts at:      0     215     329     341     357 
   Size:        215     114      12      16

  Fragments arranged by size: 

                 215     114      16      12
 
BtgI C'CryG_G

Cuts at:      0     134     149     206     357 
   Size:        134      15      57     151

  Fragments arranged by size: 

                 151     134      57      15
 
BtsI GCAGTG_nn'

Cuts at:      0      35     158     357 
   Size:         35     123     199

  Fragments arranged by size: 

                 199     123      35
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     210     245     357 
   Size:        210      35     112

  Fragments arranged by size: 

                 210     112      35
 
EaeI y'GGCC_r

Cuts at:      0      41      89     212     357 
   Size:         41      48     123     145

  Fragments arranged by size: 

                 145     123      48      41
 
MscI TGG'CCA

Cuts at:      0      43      91     357 
   Size:         43      48     266

  Fragments arranged by size: 

                 266      48      43
 
MspA1I CmG'CkG

Cuts at:      0     208     357 
   Size:        208     149
 
NcoI C'CATG_G

Cuts at:      0     134     149     357 
   Size:        134      15     208

  Fragments arranged by size: 

                 208     134      15
 
PstI C_TGCA'G

Cuts at:      0     266     357 
   Size:        266      91
 
SacII CC_GC'GG

Cuts at:      0     209     357 
   Size:        209     148
 
SfcI C'TryA_G

Cuts at:      0     101     262     357 
   Size:        101     161      95

  Fragments arranged by size: 

                 161     101      95
 
StuI AGG'CCT

Cuts at:      0       7     357 
   Size:          7     350
 
StyI C'CwwG_G

Cuts at:      0     134     149     357 
   Size:        134      15     208

  Fragments arranged by size: 

                 208     134      15
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     107     139     357 
   Size:        107      32     218

  Fragments arranged by size: 

                 218     107      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     306     357 
   Size:        306      51
 

 Enzymes that do cut:

    AlwNI     AvaI    BanII     BglI Bme1580I     BmrI    BsaWI    BsaXI
    BseRI  BsiHKAI Bsp1286I    BspEI    BsrDI     BtgI     BtsI    CspCI
     EaeI     MscI   MspA1I     NcoI     PstI    SacII     SfcI     StuI
     StyI     TstI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII
     BlpI    BmgBI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BseYI     BsgI    BsiEI    BsiWI     BsmI
    BsmBI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI
     ClaI     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MslI     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI    TaqII     TatI  Tth111I     XbaI     XhoI     XmaI     XmnI
     ZraI