Restriction map for
pF1KB8356
(Linear) (Six-base) MAPSORT of: KIBB8356.seq Check: 9658 from: 1 to: 1143
KIBB8356.orf
With 182 enzymes: SgfI *
March 3, 2008 11:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 618 1143
Size: 618 525
AccI GT'mk_AC
Cuts at: 0 566 1079 1143
Size: 566 513 64
Fragments arranged by size:
566 513 64
AflIII A'CryG_T
Cuts at: 0 935 1143
Size: 935 208
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1069 1143
Size: 1069 74
ApaI G_GGCC'C
Cuts at: 0 34 1143
Size: 34 1109
AvaI C'yCGr_G
Cuts at: 0 89 610 1143
Size: 89 521 533
Fragments arranged by size:
533 521 89
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1008 1041 1143
Size: 1008 33 102
Fragments arranged by size:
1008 102 33
BanII G_rGCy'C
Cuts at: 0 34 545 1143
Size: 34 511 598
Fragments arranged by size:
598 511 34
BbvCI CC'TCA_GC
Cuts at: 0 379 599 905 1143
Size: 379 220 306 238
Fragments arranged by size:
379 306 238 220
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 382 416 1143
Size: 382 34 727
Fragments arranged by size:
727 382 34
BglI GCCn_nnn'nGGC
Cuts at: 0 725 1143
Size: 725 418
BglII A'GATC_T
Cuts at: 0 1002 1143
Size: 1002 141
Bme1580I G_kGCm'C
Cuts at: 0 34 234 1143
Size: 34 200 909
Fragments arranged by size:
909 200 34
BmgBI CAC'GTC
Cuts at: 0 369 1143
Size: 369 774
BmrI ACTGGGnnnn_n'
Cuts at: 0 715 1143
Size: 715 428
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 540 1143
Size: 540 603
Bpu10I CC'TnA_GC
Cuts at: 0 379 599 905 1143
Size: 379 220 306 238
Fragments arranged by size:
379 306 238 220
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 164 1123 1143
Size: 164 959 20
Fragments arranged by size:
959 164 20
BsaWI w'CCGG_w
Cuts at: 0 50 1143
Size: 50 1093
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 353 383 471 501 614 644 1143
Size: 353 30 88 30 113 30 499
Fragments arranged by size:
499 353 113 88 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 374 492 553 567 605 616 922 1143
Size: 374 118 61 14 38 11 306 221
Fragments arranged by size:
374 306 221 118 61 38 14 11
BseYI C'CCAG_C
Cuts at: 0 33 1143
Size: 33 1110
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 646 936 1143
Size: 646 290 207
Fragments arranged by size:
646 290 207
BsiHKAI G_wGCw'C
Cuts at: 0 914 1143
Size: 914 229
BsmI GAATG_Cn'
Cuts at: 0 1046 1143
Size: 1046 97
Bsp1286I G_dGCh'C
Cuts at: 0 34 234 545 914 1143
Size: 34 200 311 369 229
Fragments arranged by size:
369 311 229 200 34
BspEI T'CCGG_A
Cuts at: 0 50 1143
Size: 50 1093
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 618 1143
Size: 618 525
BstXI CCAn_nnnn'nTGG
Cuts at: 0 336 1143
Size: 336 807
BstYI r'GATC_y
Cuts at: 0 983 1002 1143
Size: 983 19 141
Fragments arranged by size:
983 141 19
Bsu36I CC'TnA_GG
Cuts at: 0 296 1143
Size: 296 847
BtgI C'CryG_G
Cuts at: 0 459 1143
Size: 459 684
BtsI GCAGTG_nn'
Cuts at: 0 109 757 1143
Size: 109 648 386
Fragments arranged by size:
648 386 109
EaeI y'GGCC_r
Cuts at: 0 818 858 1143
Size: 818 40 285
Fragments arranged by size:
818 285 40
EarI CTCTTCn'nnn_
Cuts at: 0 794 1143
Size: 794 349
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 540 1143
Size: 540 603
EcoO109I rG'GnC_Cy
Cuts at: 0 30 164 1143
Size: 30 134 979
Fragments arranged by size:
979 134 30
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 672 1143
Size: 672 471
HaeII r_GCGC'y
Cuts at: 0 47 654 1143
Size: 47 607 489
Fragments arranged by size:
607 489 47
MscI TGG'CCA
Cuts at: 0 820 1143
Size: 820 323
MslI CAynn'nnrTG
Cuts at: 0 269 1051 1143
Size: 269 782 92
Fragments arranged by size:
782 269 92
MspA1I CmG'CkG
Cuts at: 0 581 1143
Size: 581 562
NspI r_CATG'y
Cuts at: 0 939 1143
Size: 939 204
PciI A'CATG_T
Cuts at: 0 935 1143
Size: 935 208
PflMI CCAn_nnn'nTGG
Cuts at: 0 518 1143
Size: 518 625
PpuMI rG'GwC_Cy
Cuts at: 0 164 1143
Size: 164 979
PspOMI G'GGCC_C
Cuts at: 0 30 1143
Size: 30 1113
SanDI GG'GwC_CC
Cuts at: 0 164 1143
Size: 164 979
SexAI A'CCwGG_T
Cuts at: 0 494 1143
Size: 494 649
SfcI C'TryA_G
Cuts at: 0 143 918 1143
Size: 143 775 225
Fragments arranged by size:
775 225 143
SmlI C'TyrA_G
Cuts at: 0 179 1138 1143
Size: 179 959 5
Fragments arranged by size:
959 179 5
StyI C'CwwG_G
Cuts at: 0 512 1143
Size: 512 631
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 353 385 471 503 614 646 757 789
Size: 353 32 86 32 111 32 111 32
Cuts at: 789 1143
Size: 354
Fragments arranged by size:
354 353 111 111 86 32 32 32
32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 973 994 1143
Size: 973 21 149
Fragments arranged by size:
973 149 21
Enzymes that do cut:
AarI AccI AflIII AlfI ApaI AvaI BaeI BanII
BbvCI BcgI BglI BglII Bme1580I BmgBI BmrI BpmI
Bpu10I BpuEI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspEI BspMI BstXI BstYI Bsu36I BtgI
BtsI EaeI EarI Eco57MI EcoO109I FalI HaeII MscI
MslI MspA1I NspI PciI PflMI PpuMI PspOMI SanDI
SexAI SfcI SmlI StyI TstI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI
AleI AloI AlwNI ApaLI ApoI AscI AseI AsiSI
AvrII BamHI BanI BbeI BbsI BciVI BclI BfrBI
BlpI BmtI BplI BsaI BsaAI BsaBI BsaHI BsiEI
BsiWI BsmBI BspHI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstZ17I BtgZI ClaI CspCI
DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI
EcoRV FseI FspI FspAI Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII TatI
Tth111I XbaI XhoI XmaI XmnI ZraI