Restriction map for
pF1KB8353
(Linear) (Six-base) MAPSORT of: KIBB8353.seq Check: 623 from: 1 to: 1041
KIBB8353.orf
With 182 enzymes: SgfI *
July 2, 2008 13:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 755 1041
Size: 755 286
AccI GT'mk_AC
Cuts at: 0 608 764 1041
Size: 608 156 277
Fragments arranged by size:
608 277 156
Acc65I G'GTAC_C
Cuts at: 0 908 1041
Size: 908 133
ApaLI G'TGCA_C
Cuts at: 0 220 1041
Size: 220 821
AvaI C'yCGr_G
Cuts at: 0 787 1041
Size: 787 254
BanI G'GyrC_C
Cuts at: 0 775 879 908 1041
Size: 775 104 29 133
Fragments arranged by size:
775 133 104 29
BanII G_rGCy'C
Cuts at: 0 248 971 1041
Size: 248 723 70
Fragments arranged by size:
723 248 70
BbvCI CC'TCA_GC
Cuts at: 0 331 1041
Size: 331 710
BclI T'GATC_A
Cuts at: 0 134 1041
Size: 134 907
BglI GCCn_nnn'nGGC
Cuts at: 0 649 827 1041
Size: 649 178 214
Fragments arranged by size:
649 214 178
Bme1580I G_kGCm'C
Cuts at: 0 224 685 884 1040 1041
Size: 224 461 199 156 1
Fragments arranged by size:
461 224 199 156 1
BmgBI CAC'GTC
Cuts at: 0 756 1041
Size: 756 285
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 531 1041
Size: 531 510
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 417 501 694 721 1041
Size: 112 305 84 193 27 320
Fragments arranged by size:
320 305 193 112 84 27
Bpu10I CC'TnA_GC
Cuts at: 0 331 1041
Size: 331 710
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 1041
Size: 232 809
BsaBI GATnn'nnATC
Cuts at: 0 545 1041
Size: 545 496
BsaWI w'CCGG_w
Cuts at: 0 857 1041
Size: 857 184
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 415 445 661 691 1041
Size: 415 30 216 30 350
Fragments arranged by size:
415 350 216 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 217 1041
Size: 217 824
BseYI C'CCAG_C
Cuts at: 0 14 395 822 988 1041
Size: 14 381 427 166 53
Fragments arranged by size:
427 381 166 53 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 1041
Size: 337 704
BsiEI CG_ry'CG
Cuts at: 0 441 1041
Size: 441 600
BsiHKAI G_wGCw'C
Cuts at: 0 224 248 746 1041
Size: 224 24 498 295
Fragments arranged by size:
498 295 224 24
BsmBI CGTCTCn'nnnn_
Cuts at: 0 999 1041
Size: 999 42
Bsp1286I G_dGCh'C
Cuts at: 0 224 248 685 746 884 971 1040 1041
Size: 224 24 437 61 138 87 69 1
Fragments arranged by size:
437 224 138 87 69 61 24 1
BspEI T'CCGG_A
Cuts at: 0 857 1041
Size: 857 184
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 218 755 1041
Size: 218 537 286
Fragments arranged by size:
537 286 218
BsrDI GCAATG_nn'
Cuts at: 0 561 932 1041
Size: 561 371 109
Fragments arranged by size:
561 371 109
BsrGI T'GTAC_A
Cuts at: 0 593 1041
Size: 593 448
BssSI C'ACGA_G
Cuts at: 0 225 1041
Size: 225 816
BstEII G'GTnAC_C
Cuts at: 0 894 1041
Size: 894 147
BstXI CCAn_nnnn'nTGG
Cuts at: 0 408 891 1041
Size: 408 483 150
Fragments arranged by size:
483 408 150
BstZ17I GTA'TAC
Cuts at: 0 765 1041
Size: 765 276
Bsu36I CC'TnA_GG
Cuts at: 0 900 1041
Size: 900 141
BtgI C'CryG_G
Cuts at: 0 441 867 1041
Size: 441 426 174
Fragments arranged by size:
441 426 174
BtsI GCAGTG_nn'
Cuts at: 0 426 1040 1041
Size: 426 614 1
Fragments arranged by size:
614 426 1
EaeI y'GGCC_r
Cuts at: 0 46 870 1041
Size: 46 824 171
Fragments arranged by size:
824 171 46
EarI CTCTTCn'nnn_
Cuts at: 0 146 1041
Size: 146 895
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 675 1041
Size: 675 366
EcoICRI GAG'CTC
Cuts at: 0 246 1041
Size: 246 795
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 417 501 694 721 1041
Size: 112 305 84 193 27 320
Fragments arranged by size:
320 305 193 112 84 27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 706 1041
Size: 706 335
EcoO109I rG'GnC_Cy
Cuts at: 0 207 335 1041
Size: 207 128 706
Fragments arranged by size:
706 207 128
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 175 273 1041
Size: 175 98 768
Fragments arranged by size:
768 175 98
HaeII r_GCGC'y
Cuts at: 0 234 1041
Size: 234 807
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 219 251 1041
Size: 219 32 790
Fragments arranged by size:
790 219 32
HindIII A'AGCT_T
Cuts at: 0 157 1041
Size: 157 884
KpnI G_GTAC'C
Cuts at: 0 912 1041
Size: 912 129
MscI TGG'CCA
Cuts at: 0 48 1041
Size: 48 993
MslI CAynn'nnrTG
Cuts at: 0 82 178 406 889 1041
Size: 82 96 228 483 152
Fragments arranged by size:
483 228 152 96 82
MspA1I CmG'CkG
Cuts at: 0 18 826 1020 1041
Size: 18 808 194 21
Fragments arranged by size:
808 194 21 18
NspI r_CATG'y
Cuts at: 0 31 177 1041
Size: 31 146 864
Fragments arranged by size:
864 146 31
PasI CC'CwG_GG
Cuts at: 0 628 1041
Size: 628 413
PfoI T'CCnGG_A
Cuts at: 0 737 1041
Size: 737 304
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 838 870 1041
Size: 838 32 171
Fragments arranged by size:
838 171 32
PpuMI rG'GwC_Cy
Cuts at: 0 207 1041
Size: 207 834
PspXI vC'TCGA_Gb
Cuts at: 0 787 1041
Size: 787 254
PstI C_TGCA'G
Cuts at: 0 1035 1041
Size: 1035 6
PvuII CAG'CTG
Cuts at: 0 18 1041
Size: 18 1023
SacI G_AGCT'C
Cuts at: 0 248 1041
Size: 248 793
SfcI C'TryA_G
Cuts at: 0 1031 1041
Size: 1031 10
SmlI C'TyrA_G
Cuts at: 0 247 787 1041
Size: 247 540 254
Fragments arranged by size:
540 254 247
SphI G_CATG'C
Cuts at: 0 177 1041
Size: 177 864
StuI AGG'CCT
Cuts at: 0 196 1041
Size: 196 845
StyI C'CwwG_G
Cuts at: 0 801 1041
Size: 801 240
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1002 1041
Size: 1002 39
TatI w'GTAC_w
Cuts at: 0 56 593 1041
Size: 56 537 448
Fragments arranged by size:
537 448 56
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 415 447 661 693 852 884 1041
Size: 415 32 214 32 159 32 157
Fragments arranged by size:
415 214 159 157 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 41 553 1041
Size: 41 512 488
Fragments arranged by size:
512 488 41
XhoI C'TCGA_G
Cuts at: 0 787 1041
Size: 787 254
Enzymes that do cut:
AarI AccI Acc65I ApaLI AvaI BanI BanII BbvCI
BclI BglI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI
BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspMI BsrDI BsrGI BssSI BstEII
BstXI BstZ17I Bsu36I BtgI BtsI EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HindIII
KpnI MscI MslI MspA1I NspI PasI PfoI PpiI
PpuMI PspXI PstI PvuII SacI SfcI SmlI SphI
StuI StyI TaqII TatI TstI XcmI XhoI
Enzymes that do not cut:
AatII AclI AcuI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbsI BcgI BciVI
BfrBI BglII BlpI BmrI BmtI BsaI BsaAI BsaHI
BsiWI BsmI BspHI BsrBI BsrFI BssHII BstAPI BstBI
BstYI BtgZI ClaI CspCI DraI DraIII DrdI EagI
EcoRI EcoRV FseI FspI FspAI HincII HpaI KasI
MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PflMI PmeI
PmlI PshAI PsiI PspOMI PsrI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI
Tth111I XbaI XmaI XmnI ZraI