Restriction map for
pF1KB8347
(Linear) (Six-base) MAPSORT of: KIBB8347.seq Check: 3714 from: 1 to: 1134
KIBB8347.orf
With 182 enzymes: SgfI *
March 3, 2008 11:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 612 1134
Size: 612 522
AccI GT'mk_AC
Cuts at: 0 890 1134
Size: 890 244
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 354 1134
Size: 223 131 780
Fragments arranged by size:
780 223 131
AfeI AGC'GCT
Cuts at: 0 1003 1134
Size: 1003 131
AlwNI CAG_nnn'CTG
Cuts at: 0 158 270 303 948 1075 1134
Size: 158 112 33 645 127 59
Fragments arranged by size:
645 158 127 112 59 33
ApaLI G'TGCA_C
Cuts at: 0 608 1134
Size: 608 526
AvaI C'yCGr_G
Cuts at: 0 104 397 689 1134
Size: 104 293 292 445
Fragments arranged by size:
445 293 292 104
BbsI GAAGACnn'nnnn_
Cuts at: 0 53 409 1134
Size: 53 356 725
Fragments arranged by size:
725 356 53
BglI GCCn_nnn'nGGC
Cuts at: 0 869 1048 1134
Size: 869 179 86
Fragments arranged by size:
869 179 86
BlpI GC'TnA_GC
Cuts at: 0 355 1134
Size: 355 779
Bme1580I G_kGCm'C
Cuts at: 0 612 1134
Size: 612 522
BmgBI CAC'GTC
Cuts at: 0 939 1134
Size: 939 195
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 432 1134
Size: 432 702
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 262 325 679 898 1134
Size: 232 30 63 354 219 236
Fragments arranged by size:
354 236 232 219 63 30
BsaHI Gr'CG_yC
Cuts at: 0 956 1134
Size: 956 178
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 295 322 424 436 565 613 850 853
Size: 295 27 102 12 129 48 237 3
Cuts at: 853 856 1078 1134
Size: 3 222 56
Fragments arranged by size:
295 237 222 129 102 56 48 27
12 3 3
BseYI C'CCAG_C
Cuts at: 0 290 500 1134
Size: 290 210 634
Fragments arranged by size:
634 290 210
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 961 1134
Size: 961 173
BsiEI CG_ry'CG
Cuts at: 0 735 1134
Size: 735 399
BsiHKAI G_wGCw'C
Cuts at: 0 612 1134
Size: 612 522
BsmBI CGTCTCn'nnnn_
Cuts at: 0 46 1134
Size: 46 1088
Bsp1286I G_dGCh'C
Cuts at: 0 612 1134
Size: 612 522
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 612 1060 1134
Size: 612 448 74
Fragments arranged by size:
612 448 74
BsrBI CCG'CTC
Cuts at: 0 285 1134
Size: 285 849
BstAPI GCAn_nnn'nTGC
Cuts at: 0 948 1134
Size: 948 186
BstXI CCAn_nnnn'nTGG
Cuts at: 0 567 1134
Size: 567 567
Bsu36I CC'TnA_GG
Cuts at: 0 905 1134
Size: 905 229
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1127 1134
Size: 1127 7
DraIII CAC_nnn'GTG
Cuts at: 0 543 1134
Size: 543 591
EaeI y'GGCC_r
Cuts at: 0 74 182 218 665 1134
Size: 74 108 36 447 469
Fragments arranged by size:
469 447 108 74 36
EarI CTCTTCn'nnn_
Cuts at: 0 88 322 1134
Size: 88 234 812
Fragments arranged by size:
812 234 88
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 232 262 325 354 679 898 1134
Size: 223 9 30 63 29 325 219 236
Fragments arranged by size:
325 236 223 219 63 30 29 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 904 1134
Size: 904 230
EcoO109I rG'GnC_Cy
Cuts at: 0 1026 1072 1080 1134
Size: 1026 46 8 54
Fragments arranged by size:
1026 54 46 8
FspI TGC'GCA
Cuts at: 0 274 316 808 1134
Size: 274 42 492 326
Fragments arranged by size:
492 326 274 42
HaeII r_GCGC'y
Cuts at: 0 249 1005 1134
Size: 249 756 129
Fragments arranged by size:
756 249 129
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 717 749 1134
Size: 717 32 385
Fragments arranged by size:
717 385 32
MscI TGG'CCA
Cuts at: 0 667 1134
Size: 667 467
MslI CAynn'nnrTG
Cuts at: 0 31 1134
Size: 31 1103
MspA1I CmG'CkG
Cuts at: 0 89 270 500 1134
Size: 89 181 230 634
Fragments arranged by size:
634 230 181 89
NspI r_CATG'y
Cuts at: 0 336 492 999 1134
Size: 336 156 507 135
Fragments arranged by size:
507 336 156 135
PflMI CCAn_nnn'nTGG
Cuts at: 0 744 1134
Size: 744 390
PfoI T'CCnGG_A
Cuts at: 0 656 1134
Size: 656 478
PpuMI rG'GwC_Cy
Cuts at: 0 1026 1072 1080 1134
Size: 1026 46 8 54
Fragments arranged by size:
1026 54 46 8
PshAI GACnn'nnGTC
Cuts at: 0 640 1134
Size: 640 494
PstI C_TGCA'G
Cuts at: 0 953 1134
Size: 953 181
PvuI CG_AT'CG
Cuts at: 0 735 1134
Size: 735 399
PvuII CAG'CTG
Cuts at: 0 270 1134
Size: 270 864
SanDI GG'GwC_CC
Cuts at: 0 1026 1080 1134
Size: 1026 54 54
Fragments arranged by size:
1026 54 54
SapI GCTCTTCn'nnn_
Cuts at: 0 322 1134
Size: 322 812
SexAI A'CCwGG_T
Cuts at: 0 539 566 1134
Size: 539 27 568
Fragments arranged by size:
568 539 27
SfcI C'TryA_G
Cuts at: 0 9 949 1134
Size: 9 940 185
Fragments arranged by size:
940 185 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 869 1048 1134
Size: 869 179 86
Fragments arranged by size:
869 179 86
SmaI CCC'GGG
Cuts at: 0 106 691 1134
Size: 106 585 443
Fragments arranged by size:
585 443 106
SphI G_CATG'C
Cuts at: 0 336 492 1134
Size: 336 156 642
Fragments arranged by size:
642 336 156
StyI C'CwwG_G
Cuts at: 0 1052 1134
Size: 1052 82
TatI w'GTAC_w
Cuts at: 0 740 1134
Size: 740 394
Tth111I GACn'n_nGTC
Cuts at: 0 532 880 1134
Size: 532 348 254
Fragments arranged by size:
532 348 254
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 966 1134
Size: 966 168
XmaI C'CCGG_G
Cuts at: 0 104 689 1134
Size: 104 585 445
Fragments arranged by size:
585 445 104
XmnI GAAnn'nnTTC
Cuts at: 0 922 1097 1134
Size: 922 175 37
Fragments arranged by size:
922 175 37
Enzymes that do cut:
AarI AccI AcuI AfeI AlwNI ApaLI AvaI BbsI
BglI BlpI Bme1580I BmgBI BplI BpmI BsaHI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI
BstAPI BstXI Bsu36I BtgZI DraIII EaeI EarI Eco57MI
EcoNI EcoO109I FspI HaeII Hin4I MscI MslI MspA1I
NspI PflMI PfoI PpuMI PshAI PstI PvuI PvuII
SanDI SapI SexAI SfcI SfiI SmaI SphI StyI
TatI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AleI
AlfI AloI ApaI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BanI BanII BbeI BbvCI BcgI BciVI
BclI BfrBI BglII BmrI BmtI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaWI BsaXI BsiWI BsmI BspEI BspHI
BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII BstYI
BstZ17I BtgI BtsI ClaI CspCI DraI DrdI EagI
EciI EcoICRI EcoRI EcoRV FalI FseI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PmeI PmlI PpiI PsiI PspOMI
PspXI PsrI RsrII SacI SacII SalI SbfI ScaI
SfoI SgfI SgrAI SmlI SnaBI SpeI SrfI SspI
StuI SwaI TaqII TstI XbaI XhoI ZraI