Restriction map for
pF1KB8344
(Linear) (Six-base) MAPSORT of: KIBB8344.seq Check: 4219 from: 1 to: 903
KIBB8344.orf
With 182 enzymes: SgfI *
March 3, 2008 11:05 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 382 903
Size: 382 521
AflIII A'CryG_T
Cuts at: 0 868 903
Size: 868 35
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 139 903
Size: 139 764
AlwNI CAG_nnn'CTG
Cuts at: 0 136 903
Size: 136 767
ApaI G_GGCC'C
Cuts at: 0 467 903
Size: 467 436
ApoI r'AATT_y
Cuts at: 0 808 903
Size: 808 95
BanI G'GyrC_C
Cuts at: 0 197 667 903
Size: 197 470 236
Fragments arranged by size:
470 236 197
BanII G_rGCy'C
Cuts at: 0 467 658 903
Size: 467 191 245
Fragments arranged by size:
467 245 191
BbeI G_GCGC'C
Cuts at: 0 201 671 903
Size: 201 470 232
Fragments arranged by size:
470 232 201
BclI T'GATC_A
Cuts at: 0 185 260 903
Size: 185 75 643
Fragments arranged by size:
643 185 75
BglII A'GATC_T
Cuts at: 0 488 903
Size: 488 415
BlpI GC'TnA_GC
Cuts at: 0 137 903
Size: 137 766
Bme1580I G_kGCm'C
Cuts at: 0 108 467 903
Size: 108 359 436
Fragments arranged by size:
436 359 108
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 477 787 903
Size: 316 161 310 116
Fragments arranged by size:
316 310 161 116
BsaAI yAC'GTr
Cuts at: 0 869 903
Size: 869 34
BsaHI Gr'CG_yC
Cuts at: 0 198 668 903
Size: 198 470 235
Fragments arranged by size:
470 235 198
BsaWI w'CCGG_w
Cuts at: 0 419 903
Size: 419 484
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 223 253 903
Size: 223 30 650
Fragments arranged by size:
650 223 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 247 319 322 903
Size: 247 72 3 581
Fragments arranged by size:
581 247 72 3
BseYI C'CCAG_C
Cuts at: 0 24 108 627 762 903
Size: 24 84 519 135 141
Fragments arranged by size:
519 141 135 84 24
BsiHKAI G_wGCw'C
Cuts at: 0 658 903
Size: 658 245
BsmI GAATG_Cn'
Cuts at: 0 572 696 903
Size: 572 124 207
Fragments arranged by size:
572 207 124
Bsp1286I G_dGCh'C
Cuts at: 0 108 467 658 903
Size: 108 359 191 245
Fragments arranged by size:
359 245 191 108
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 275 468 903
Size: 275 193 435
Fragments arranged by size:
435 275 193
BssSI C'ACGA_G
Cuts at: 0 79 903
Size: 79 824
BstAPI GCAn_nnn'nTGC
Cuts at: 0 608 903
Size: 608 295
BstXI CCAn_nnnn'nTGG
Cuts at: 0 811 903
Size: 811 92
BstYI r'GATC_y
Cuts at: 0 488 903
Size: 488 415
BtsI GCAGTG_nn'
Cuts at: 0 63 244 903
Size: 63 181 659
Fragments arranged by size:
659 181 63
DrdI GACnn_nn'nnGTC
Cuts at: 0 285 903
Size: 285 618
EarI CTCTTCn'nnn_
Cuts at: 0 296 395 431 903
Size: 296 99 36 472
Fragments arranged by size:
472 296 99 36
EcoICRI GAG'CTC
Cuts at: 0 656 903
Size: 656 247
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 382 477 787 903
Size: 316 66 95 310 116
Fragments arranged by size:
316 310 116 95 66
EcoO109I rG'GnC_Cy
Cuts at: 0 287 463 464 903
Size: 287 176 1 439
Fragments arranged by size:
439 287 176 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 852 903
Size: 852 51
HaeII r_GCGC'y
Cuts at: 0 201 671 903
Size: 201 470 232
Fragments arranged by size:
470 232 201
HincII GTy'rAC
Cuts at: 0 165 903
Size: 165 738
HindIII A'AGCT_T
Cuts at: 0 794 903
Size: 794 109
HpaI GTT'AAC
Cuts at: 0 165 903
Size: 165 738
KasI G'GCGC_C
Cuts at: 0 197 667 903
Size: 197 470 236
Fragments arranged by size:
470 236 197
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 194 903
Size: 194 709
MslI CAynn'nnrTG
Cuts at: 0 296 560 903
Size: 296 264 343
Fragments arranged by size:
343 296 264
MspA1I CmG'CkG
Cuts at: 0 631 762 903
Size: 631 131 141
Fragments arranged by size:
631 141 131
NarI GG'CG_CC
Cuts at: 0 198 668 903
Size: 198 470 235
Fragments arranged by size:
470 235 198
PfoI T'CCnGG_A
Cuts at: 0 332 803 903
Size: 332 471 100
Fragments arranged by size:
471 332 100
PmlI CAC'GTG
Cuts at: 0 869 903
Size: 869 34
PpuMI rG'GwC_Cy
Cuts at: 0 287 903
Size: 287 616
PspOMI G'GGCC_C
Cuts at: 0 463 903
Size: 463 440
PstI C_TGCA'G
Cuts at: 0 41 132 413 903
Size: 41 91 281 490
Fragments arranged by size:
490 281 91 41
PvuII CAG'CTG
Cuts at: 0 631 762 903
Size: 631 131 141
Fragments arranged by size:
631 141 131
SacI G_AGCT'C
Cuts at: 0 658 903
Size: 658 245
ScaI AGT'ACT
Cuts at: 0 43 903
Size: 43 860
SfcI C'TryA_G
Cuts at: 0 37 48 128 409 903
Size: 37 11 80 281 494
Fragments arranged by size:
494 281 80 37 11
SfoI GGC'GCC
Cuts at: 0 199 669 903
Size: 199 470 234
Fragments arranged by size:
470 234 199
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 107 598 903
Size: 107 491 305
Fragments arranged by size:
491 305 107
TatI w'GTAC_w
Cuts at: 0 41 903
Size: 41 862
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 223 255 903
Size: 223 32 648
Fragments arranged by size:
648 223 32
XmnI GAAnn'nnTTC
Cuts at: 0 404 797 903
Size: 404 393 106
Fragments arranged by size:
404 393 106
Enzymes that do cut:
AcuI AflIII AlfI AlwNI ApaI ApoI BanI BanII
BbeI BclI BglII BlpI Bme1580I BpmI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I BspMI
BssSI BstAPI BstXI BstYI BtsI DrdI EarI EcoICRI
Eco57MI EcoO109I FalI HaeII HincII HindIII HpaI KasI
MmeI MslI MspA1I NarI PfoI PmlI PpuMI PspOMI
PstI PvuII SacI ScaI SfcI SfoI TaqII TatI
TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AloI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BfrBI
BglI BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaI
BsaBI BsgI BsiEI BsiWI BsmBI BspEI BspHI BsrBI
BsrDI BsrFI BsrGI BssHII BstBI BstEII BstZ17I Bsu36I
BtgI BtgZI ClaI CspCI DraI DraIII EaeI EagI
EciI EcoNI EcoRI EcoRV FseI FspI FspAI Hin4I
KpnI MfeI MluI MscI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI SbfI SexAI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI Tth111I XbaI XcmI XhoI
XmaI ZraI