Restriction map for
pF1KB8342
(Linear) (Six-base) MAPSORT of: KIBB8342.seq Check: 2896 from: 1 to: 1044
KIBB8342.orf
With 182 enzymes: SgfI *
March 3, 2008 11:05 ..
AflIII A'CryG_T
Cuts at: 0 128 617 1044
Size: 128 489 427
Fragments arranged by size:
489 427 128
ApaI G_GGCC'C
Cuts at: 0 320 1044
Size: 320 724
AvaI C'yCGr_G
Cuts at: 0 538 1044
Size: 538 506
AvrII C'CTAG_G
Cuts at: 0 261 1044
Size: 261 783
BanI G'GyrC_C
Cuts at: 0 795 1044
Size: 795 249
BanII G_rGCy'C
Cuts at: 0 320 841 1044
Size: 320 521 203
Fragments arranged by size:
521 320 203
BbsI GAAGACnn'nnnn_
Cuts at: 0 303 672 1044
Size: 303 369 372
Fragments arranged by size:
372 369 303
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 51 85 1044
Size: 51 34 959
Fragments arranged by size:
959 51 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 295 1044
Size: 295 749
BglI GCCn_nnn'nGGC
Cuts at: 0 811 1044
Size: 811 233
BlpI GC'TnA_GC
Cuts at: 0 95 1044
Size: 95 949
Bme1580I G_kGCm'C
Cuts at: 0 35 320 800 1044
Size: 35 285 480 244
Fragments arranged by size:
480 285 244 35
BmrI ACTGGGnnnn_n'
Cuts at: 0 991 1044
Size: 991 53
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 565 1044
Size: 565 479
BsaAI yAC'GTr
Cuts at: 0 30 408 1044
Size: 30 378 636
Fragments arranged by size:
636 378 30
BsaBI GATnn'nnATC
Cuts at: 0 652 1044
Size: 652 392
BsaWI w'CCGG_w
Cuts at: 0 938 1044
Size: 938 106
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 823 853 1044
Size: 823 30 191
Fragments arranged by size:
823 191 30
BseYI C'CCAG_C
Cuts at: 0 147 704 791 870 1044
Size: 147 557 87 79 174
Fragments arranged by size:
557 174 147 87 79
BsiHKAI G_wGCw'C
Cuts at: 0 556 1044
Size: 556 488
BsmI GAATG_Cn'
Cuts at: 0 814 1044
Size: 814 230
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1015 1044
Size: 1015 29
Bsp1286I G_dGCh'C
Cuts at: 0 35 320 556 800 841 1044
Size: 35 285 236 244 41 203
Fragments arranged by size:
285 244 236 203 41 35
BspEI T'CCGG_A
Cuts at: 0 938 1044
Size: 938 106
BspHI T'CATG_A
Cuts at: 0 860 920 1044
Size: 860 60 124
Fragments arranged by size:
860 124 60
BsrBI CCG'CTC
Cuts at: 0 402 1044
Size: 402 642
BsrFI r'CCGG_y
Cuts at: 0 517 1044
Size: 517 527
BssSI C'ACGA_G
Cuts at: 0 555 1044
Size: 555 489
BstEII G'GTnAC_C
Cuts at: 0 747 1044
Size: 747 297
BtgI C'CryG_G
Cuts at: 0 100 239 1044
Size: 100 139 805
Fragments arranged by size:
805 139 100
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 759 1044
Size: 759 285
BtsI GCAGTG_nn'
Cuts at: 0 556 1044
Size: 556 488
DraIII CAC_nnn'GTG
Cuts at: 0 985 1044
Size: 985 59
EaeI y'GGCC_r
Cuts at: 0 119 392 1044
Size: 119 273 652
Fragments arranged by size:
652 273 119
EarI CTCTTCn'nnn_
Cuts at: 0 256 1044
Size: 256 788
EcoO109I rG'GnC_Cy
Cuts at: 0 16 543 1044
Size: 16 527 501
Fragments arranged by size:
527 501 16
FspI TGC'GCA
Cuts at: 0 205 1044
Size: 205 839
HaeII r_GCGC'y
Cuts at: 0 493 893 1044
Size: 493 400 151
Fragments arranged by size:
493 400 151
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 293 325 982 1014 1044
Size: 293 32 657 32 30
Fragments arranged by size:
657 293 32 32 30
HincII GTy'rAC
Cuts at: 0 594 1044
Size: 594 450
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 866 1044
Size: 866 178
MscI TGG'CCA
Cuts at: 0 394 1044
Size: 394 650
MslI CAynn'nnrTG
Cuts at: 0 616 622 643 754 766 859 1044
Size: 616 6 21 111 12 93 185
Fragments arranged by size:
616 185 111 93 21 12 6
MspA1I CmG'CkG
Cuts at: 0 700 1044
Size: 700 344
NaeI GCC'GGC
Cuts at: 0 519 1044
Size: 519 525
NcoI C'CATG_G
Cuts at: 0 100 1044
Size: 100 944
NgoMIV G'CCGG_C
Cuts at: 0 517 1044
Size: 517 527
NspI r_CATG'y
Cuts at: 0 132 621 951 1044
Size: 132 489 330 93
Fragments arranged by size:
489 330 132 93
PasI CC'CwG_GG
Cuts at: 0 354 1044
Size: 354 690
PciI A'CATG_T
Cuts at: 0 128 617 1044
Size: 128 489 427
Fragments arranged by size:
489 427 128
PspOMI G'GGCC_C
Cuts at: 0 316 1044
Size: 316 728
PstI C_TGCA'G
Cuts at: 0 551 1044
Size: 551 493
ScaI AGT'ACT
Cuts at: 0 85 1044
Size: 85 959
SfcI C'TryA_G
Cuts at: 0 547 1044
Size: 547 497
SmlI C'TyrA_G
Cuts at: 0 580 1044
Size: 580 464
SphI G_CATG'C
Cuts at: 0 951 1044
Size: 951 93
StuI AGG'CCT
Cuts at: 0 997 1044
Size: 997 47
StyI C'CwwG_G
Cuts at: 0 100 261 380 1044
Size: 100 161 119 664
Fragments arranged by size:
664 161 119 100
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 783 838 844 973 1044
Size: 783 55 6 129 71
Fragments arranged by size:
783 129 71 55 6
TatI w'GTAC_w
Cuts at: 0 83 1044
Size: 83 961
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 823 855 1044
Size: 823 32 189
Fragments arranged by size:
823 189 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 518 1044
Size: 518 526
Enzymes that do cut:
AflIII ApaI AvaI AvrII BanI BanII BbsI BcgI
BciVI BglI BlpI Bme1580I BmrI BpuEI BsaAI BsaBI
BsaWI BsaXI BseYI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BsrBI BsrFI BssSI BstEII BtgI BtgZI BtsI
DraIII EaeI EarI EcoO109I FspI HaeII Hin4I HincII
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI
PasI PciI PspOMI PstI ScaI SfcI SmlI SphI
StuI StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AscI AseI AsiSI BaeI BamHI BbeI BbvCI BclI
BfrBI BglII BmgBI BmtI BplI BpmI Bpu10I BsaI
BsaHI BseRI BsgI BsiEI BsiWI BspMI BsrDI BsrGI
BssHII BstAPI BstBI BstXI BstYI BstZ17I Bsu36I ClaI
CspCI DraI DrdI EagI EciI EcoICRI Eco57MI EcoNI
EcoRI EcoRV FalI FseI FspAI HindIII HpaI KasI
KpnI MfeI MluI NarI NdeI NheI NotI NruI
NsiI PacI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI
Tth111I XbaI XhoI XmaI XmnI ZraI