(Linear) (Six-base) MAPSORT of: KIBB8340.seq  Check: 640  from: 1  to: 219



KIBB8340.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:05  ..

AccI GT'mk_AC

Cuts at:      0      71     219 
   Size:         71     148
 
AlwNI CAG_nnn'CTG

Cuts at:      0     162     219 
   Size:        162      57
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70     219 
   Size:         70     149
 
BsaI GGTCTCn'nnnn_

Cuts at:      0       4     130     219 
   Size:          4     126      89

  Fragments arranged by size: 

                 126      89       4
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     145     148     219 
   Size:        145       3      71

  Fragments arranged by size: 

                 145      71       3
 
BsiEI CG_ry'CG

Cuts at:      0     113     219 
   Size:        113     106
 
BsrBI CCG'CTC

Cuts at:      0      20     219 
   Size:         20     199
 
BstYI r'GATC_y

Cuts at:      0     205     219 
   Size:        205      14
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      90     219 
   Size:         90     129
 
DraIII CAC_nnn'GTG

Cuts at:      0      98     219 
   Size:         98     121
 
EaeI y'GGCC_r

Cuts at:      0     110     219 
   Size:        110     109
 
EagI C'GGCC_G

Cuts at:      0     110     219 
   Size:        110     109
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      22     219 
   Size:         22     197
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      70     219 
   Size:         70     149
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     127     219 
   Size:        127      92
 
HaeII r_GCGC'y

Cuts at:      0     162     180     219 
   Size:        162      18      39

  Fragments arranged by size: 

                 162      39      18
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     190     219 
   Size:        190      29
 
HincII GTy'rAC

Cuts at:      0      72     219 
   Size:         72     147
 
PfoI T'CCnGG_A

Cuts at:      0      47     219 
   Size:         47     172
 
SalI G'TCGA_C

Cuts at:      0      70     219 
   Size:         70     149
 
SexAI A'CCwGG_T

Cuts at:      0      35     219 
   Size:         35     184
 
StuI AGG'CCT

Cuts at:      0     122     188     219 
   Size:        122      66      31

  Fragments arranged by size: 

                 122      66      31
 
TatI w'GTAC_w

Cuts at:      0      53     219 
   Size:         53     166
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      70     102     219 
   Size:         70      32     117

  Fragments arranged by size: 

                 117      70      32
 

 Enzymes that do cut:

     AccI    AlwNI     BpmI     BsaI    BseRI    BsiEI    BsrBI    BstYI
    BtgZI   DraIII     EaeI     EagI     EciI  Eco57MI    EcoNI    HaeII
    Hin4I   HincII     PfoI     SalI    SexAI     StuI     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI
     BsgI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI     BtsI     ClaI    CspCI
     DraI     DrdI     EarI  EcoICRI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII    SanDI     SapI     SbfI     ScaI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StyI     SwaI    TaqII  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI