Restriction map for
pF1KB8339
(Linear) (Six-base) MAPSORT of: KIBB8339.seq Check: 4935 from: 1 to: 930
KIBB8339.orf
With 182 enzymes: SgfI *
March 3, 2008 11:05 ..
Acc65I G'GTAC_C
Cuts at: 0 388 930
Size: 388 542
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 806 930
Size: 806 124
AfeI AGC'GCT
Cuts at: 0 526 930
Size: 526 404
AleI CACnn'nnGTG
Cuts at: 0 367 930
Size: 367 563
AlwNI CAG_nnn'CTG
Cuts at: 0 334 412 930
Size: 334 78 518
Fragments arranged by size:
518 334 78
AvaI C'yCGr_G
Cuts at: 0 584 930
Size: 584 346
AvrII C'CTAG_G
Cuts at: 0 538 930
Size: 538 392
BanI G'GyrC_C
Cuts at: 0 235 388 890 930
Size: 235 153 502 40
Fragments arranged by size:
502 235 153 40
BanII G_rGCy'C
Cuts at: 0 433 606 882 930
Size: 433 173 276 48
Fragments arranged by size:
433 276 173 48
BbeI G_GCGC'C
Cuts at: 0 239 930
Size: 239 691
BbsI GAAGACnn'nnnn_
Cuts at: 0 83 141 930
Size: 83 58 789
Fragments arranged by size:
789 83 58
BglII A'GATC_T
Cuts at: 0 849 930
Size: 849 81
BlpI GC'TnA_GC
Cuts at: 0 605 930
Size: 605 325
BmgBI CAC'GTC
Cuts at: 0 828 930
Size: 828 102
BmrI ACTGGGnnnn_n'
Cuts at: 0 80 930
Size: 80 850
BsaHI Gr'CG_yC
Cuts at: 0 236 930
Size: 236 694
BseYI C'CCAG_C
Cuts at: 0 358 444 930
Size: 358 86 486
Fragments arranged by size:
486 358 86
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 80 679 930
Size: 64 16 599 251
Fragments arranged by size:
599 251 64 16
BsiHKAI G_wGCw'C
Cuts at: 0 700 882 930
Size: 700 182 48
Fragments arranged by size:
700 182 48
BsmBI CGTCTCn'nnnn_
Cuts at: 0 331 930
Size: 331 599
Bsp1286I G_dGCh'C
Cuts at: 0 433 606 700 882 930
Size: 433 173 94 182 48
Fragments arranged by size:
433 182 173 94 48
BsrGI T'GTAC_A
Cuts at: 0 179 930
Size: 179 751
BstYI r'GATC_y
Cuts at: 0 849 930
Size: 849 81
BtgI C'CryG_G
Cuts at: 0 894 930
Size: 894 36
BtsI GCAGTG_nn'
Cuts at: 0 68 374 439 930
Size: 68 306 65 491
Fragments arranged by size:
491 306 68 65
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 71 106 930
Size: 71 35 824
Fragments arranged by size:
824 71 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 136 930
Size: 136 794
EaeI y'GGCC_r
Cuts at: 0 216 708 930
Size: 216 492 222
Fragments arranged by size:
492 222 216
EarI CTCTTCn'nnn_
Cuts at: 0 26 930
Size: 26 904
EcoICRI GAG'CTC
Cuts at: 0 880 930
Size: 880 50
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 806 930
Size: 806 124
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 165 930
Size: 165 765
HaeII r_GCGC'y
Cuts at: 0 239 528 930
Size: 239 289 402
Fragments arranged by size:
402 289 239
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 626 658 890 922 930
Size: 626 32 232 32 8
Fragments arranged by size:
626 232 32 32 8
HincII GTy'rAC
Cuts at: 0 315 930
Size: 315 615
HindIII A'AGCT_T
Cuts at: 0 737 930
Size: 737 193
KasI G'GCGC_C
Cuts at: 0 235 930
Size: 235 695
KpnI G_GTAC'C
Cuts at: 0 392 930
Size: 392 538
MfeI C'AATT_G
Cuts at: 0 530 930
Size: 530 400
MscI TGG'CCA
Cuts at: 0 218 710 930
Size: 218 492 220
Fragments arranged by size:
492 220 218
MslI CAynn'nnrTG
Cuts at: 0 367 930
Size: 367 563
MspA1I CmG'CkG
Cuts at: 0 22 115 210 331 409 816 930
Size: 22 93 95 121 78 407 114
Fragments arranged by size:
407 121 114 95 93 78 22
NarI GG'CG_CC
Cuts at: 0 236 930
Size: 236 694
PasI CC'CwG_GG
Cuts at: 0 426 930
Size: 426 504
PfoI T'CCnGG_A
Cuts at: 0 752 930
Size: 752 178
PstI C_TGCA'G
Cuts at: 0 512 797 815 930
Size: 512 285 18 115
Fragments arranged by size:
512 285 115 18
PvuII CAG'CTG
Cuts at: 0 22 115 210 331 409 816 930
Size: 22 93 95 121 78 407 114
Fragments arranged by size:
407 121 114 95 93 78 22
SacI G_AGCT'C
Cuts at: 0 882 930
Size: 882 48
ScaI AGT'ACT
Cuts at: 0 506 930
Size: 506 424
SfcI C'TryA_G
Cuts at: 0 508 793 811 930
Size: 508 285 18 119
Fragments arranged by size:
508 285 119 18
SfoI GGC'GCC
Cuts at: 0 237 930
Size: 237 693
StyI C'CwwG_G
Cuts at: 0 538 930
Size: 538 392
TatI w'GTAC_w
Cuts at: 0 179 403 504 930
Size: 179 224 101 426
Fragments arranged by size:
426 224 179 101
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 538 570 930
Size: 538 32 360
Fragments arranged by size:
538 360 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 366 718 930
Size: 366 352 212
Fragments arranged by size:
366 352 212
Enzymes that do cut:
Acc65I AcuI AfeI AleI AlwNI AvaI AvrII BanI
BanII BbeI BbsI BglII BlpI BmgBI BmrI BsaHI
BseYI BsgI BsiHKAI BsmBI Bsp1286I BsrGI BstYI BtgI
BtsI CspCI DrdI EaeI EarI EcoICRI Eco57MI FalI
HaeII Hin4I HincII HindIII KasI KpnI MfeI MscI
MslI MspA1I NarI PasI PfoI PstI PvuII SacI
ScaI SfcI SfoI StyI TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AflIII AgeI AhdI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
BaeI BamHI BbvCI BcgI BciVI BclI BfrBI BglI
Bme1580I BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaWI BsaXI BseRI BsiEI BsiWI BsmI BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI DraI
DraIII EagI EciI EcoNI EcoO109I EcoRI EcoRV FseI
FspI FspAI HpaI MluI MmeI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SapI
SbfI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII Tth111I
XbaI XhoI XmaI XmnI ZraI