Restriction map for
pF1KB8338
(Linear) (Six-base) MAPSORT of: KIBB8338.seq Check: 9629 from: 1 to: 816
KIBB8338.orf
With 182 enzymes: SgfI *
March 3, 2008 11:05 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 135 816
Size: 135 681
AflIII A'CryG_T
Cuts at: 0 683 816
Size: 683 133
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 337 816
Size: 337 479
AlwNI CAG_nnn'CTG
Cuts at: 0 654 816
Size: 654 162
ApaI G_GGCC'C
Cuts at: 0 18 816
Size: 18 798
ApaLI G'TGCA_C
Cuts at: 0 208 274 816
Size: 208 66 542
Fragments arranged by size:
542 208 66
BanI G'GyrC_C
Cuts at: 0 52 190 220 285 303 517 629 816
Size: 52 138 30 65 18 214 112 187
Fragments arranged by size:
214 187 138 112 65 52 30 18
BanII G_rGCy'C
Cuts at: 0 18 341 816
Size: 18 323 475
Fragments arranged by size:
475 323 18
BbeI G_GCGC'C
Cuts at: 0 56 194 224 289 307 521 816
Size: 56 138 30 65 18 214 295
Fragments arranged by size:
295 214 138 65 56 30 18
BglI GCCn_nnn'nGGC
Cuts at: 0 117 816
Size: 117 699
BglII A'GATC_T
Cuts at: 0 752 816
Size: 752 64
BlpI GC'TnA_GC
Cuts at: 0 73 816
Size: 73 743
Bme1580I G_kGCm'C
Cuts at: 0 18 212 278 634 816
Size: 18 194 66 356 182
Fragments arranged by size:
356 194 182 66 18
BmrI ACTGGGnnnn_n'
Cuts at: 0 20 199 816
Size: 20 179 617
Fragments arranged by size:
617 179 20
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 355 360 470 682 816
Size: 355 5 110 212 134
Fragments arranged by size:
355 212 134 110 5
BsaAI yAC'GTr
Cuts at: 0 33 273 816
Size: 33 240 543
Fragments arranged by size:
543 240 33
BsaHI Gr'CG_yC
Cuts at: 0 53 59 191 221 286 304 518 816
Size: 53 6 132 30 65 18 214 298
Fragments arranged by size:
298 214 132 65 53 30 18 6
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 310 340 655 685 816
Size: 310 30 315 30 131
Fragments arranged by size:
315 310 131 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 473 816
Size: 473 343
BseYI C'CCAG_C
Cuts at: 0 48 508 585 816
Size: 48 460 77 231
Fragments arranged by size:
460 231 77 48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 180 816
Size: 180 636
BsiHKAI G_wGCw'C
Cuts at: 0 143 212 278 816
Size: 143 69 66 538
Fragments arranged by size:
538 143 69 66
Bsp1286I G_dGCh'C
Cuts at: 0 18 143 212 278 341 634 816
Size: 18 125 69 66 63 293 182
Fragments arranged by size:
293 182 125 69 66 63 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 305 695 735 816
Size: 305 390 40 81
Fragments arranged by size:
390 305 81 40
BsrBI CCG'CTC
Cuts at: 0 551 816
Size: 551 265
BsrFI r'CCGG_y
Cuts at: 0 380 816
Size: 380 436
BssHII G'CGCG_C
Cuts at: 0 607 816
Size: 607 209
BssSI C'ACGA_G
Cuts at: 0 195 816
Size: 195 621
BstYI r'GATC_y
Cuts at: 0 752 816
Size: 752 64
BtgI C'CryG_G
Cuts at: 0 62 290 816
Size: 62 228 526
Fragments arranged by size:
526 228 62
ClaI AT'CG_AT
Cuts at: 0 572 816
Size: 572 244
EaeI y'GGCC_r
Cuts at: 0 2 81 134 413 816
Size: 2 79 53 279 403
Fragments arranged by size:
403 279 79 53 2
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 569 816
Size: 569 247
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 135 355 360 470 682 816
Size: 135 220 5 110 212 134
Fragments arranged by size:
220 212 135 134 110 5
EcoO109I rG'GnC_Cy
Cuts at: 0 14 15 98 816
Size: 14 1 83 718
Fragments arranged by size:
718 83 14 1
HaeII r_GCGC'y
Cuts at: 0 56 194 224 289 307 521 816
Size: 56 138 30 65 18 214 295
Fragments arranged by size:
295 214 138 65 56 30 18
KasI G'GCGC_C
Cuts at: 0 52 190 220 285 303 517 816
Size: 52 138 30 65 18 214 299
Fragments arranged by size:
299 214 138 65 52 30 18
MscI TGG'CCA
Cuts at: 0 4 816
Size: 4 812
MspA1I CmG'CkG
Cuts at: 0 64 238 408 816
Size: 64 174 170 408
Fragments arranged by size:
408 174 170 64
NaeI GCC'GGC
Cuts at: 0 382 816
Size: 382 434
NarI GG'CG_CC
Cuts at: 0 53 191 221 286 304 518 816
Size: 53 138 30 65 18 214 298
Fragments arranged by size:
298 214 138 65 53 30 18
NgoMIV G'CCGG_C
Cuts at: 0 380 816
Size: 380 436
NspI r_CATG'y
Cuts at: 0 324 687 816
Size: 324 363 129
Fragments arranged by size:
363 324 129
PasI CC'CwG_GG
Cuts at: 0 18 102 816
Size: 18 84 714
Fragments arranged by size:
714 84 18
PciI A'CATG_T
Cuts at: 0 683 816
Size: 683 133
PflMI CCAn_nnn'nTGG
Cuts at: 0 654 816
Size: 654 162
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 661 693 816
Size: 661 32 123
Fragments arranged by size:
661 123 32
PpuMI rG'GwC_Cy
Cuts at: 0 98 816
Size: 98 718
PspOMI G'GGCC_C
Cuts at: 0 14 816
Size: 14 802
SacII CC_GC'GG
Cuts at: 0 65 816
Size: 65 751
SanDI GG'GwC_CC
Cuts at: 0 98 816
Size: 98 718
SfoI GGC'GCC
Cuts at: 0 54 192 222 287 305 519 816
Size: 54 138 30 65 18 214 297
Fragments arranged by size:
297 214 138 65 54 30 18
StyI C'CwwG_G
Cuts at: 0 173 816
Size: 173 643
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 815 816
Size: 815 1
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 439 471 816
Size: 439 32 345
Fragments arranged by size:
439 345 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 15 593 816
Size: 15 578 223
Fragments arranged by size:
578 223 15
Enzymes that do cut:
AcuI AflIII AlfI AlwNI ApaI ApaLI BanI BanII
BbeI BglI BglII BlpI Bme1580I BmrI BpmI BsaAI
BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI
BsrBI BsrFI BssHII BssSI BstYI BtgI ClaI EaeI
EciI Eco57MI EcoO109I HaeII KasI MscI MspA1I NaeI
NarI NgoMIV NspI PasI PciI PflMI PpiI PpuMI
PspOMI SacII SanDI SfoI StyI TaqII TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AloI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI
BfrBI BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaBI
BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrDI
BsrGI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI
BtsI CspCI DraI DraIII DrdI EagI EarI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MslI
NcoI NdeI NheI NotI NruI NsiI PacI PfoI
PmeI PmlI PshAI PsiI PspXI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TatI Tth111I XbaI
XhoI XmaI XmnI ZraI