Restriction map for
pF1KB8335
(Linear) (Six-base) MAPSORT of: KIBB8335.seq Check: 3729 from: 1 to: 1320
KIBB8335.orf
With 182 enzymes: SgfI *
July 2, 2008 13:23 ..
AccI GT'mk_AC
Cuts at: 0 413 1320
Size: 413 907
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 574 1320
Size: 56 518 746
Fragments arranged by size:
746 518 56
AfeI AGC'GCT
Cuts at: 0 1210 1320
Size: 1210 110
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 520 1070 1320
Size: 520 550 250
Fragments arranged by size:
550 520 250
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 343 375 1320
Size: 343 32 945
Fragments arranged by size:
945 343 32
AlwNI CAG_nnn'CTG
Cuts at: 0 180 513 1101 1320
Size: 180 333 588 219
Fragments arranged by size:
588 333 219 180
ApaLI G'TGCA_C
Cuts at: 0 319 1320
Size: 319 1001
ApoI r'AATT_y
Cuts at: 0 538 1320
Size: 538 782
AvaI C'yCGr_G
Cuts at: 0 25 1320
Size: 25 1295
BamHI G'GATC_C
Cuts at: 0 484 1320
Size: 484 836
BanII G_rGCy'C
Cuts at: 0 85 170 1320
Size: 85 85 1150
Fragments arranged by size:
1150 85 85
BbsI GAAGACnn'nnnn_
Cuts at: 0 398 1320
Size: 398 922
BlpI GC'TnA_GC
Cuts at: 0 628 763 811 1320
Size: 628 135 48 509
Fragments arranged by size:
628 509 135 48
Bme1580I G_kGCm'C
Cuts at: 0 323 1320
Size: 323 997
BmrI ACTGGGnnnn_n'
Cuts at: 0 1275 1320
Size: 1275 45
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 99 1320
Size: 99 1221
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 688 702 1042 1054 1148 1320
Size: 136 552 14 340 12 94 172
Fragments arranged by size:
552 340 172 136 94 14 12
Bpu10I CC'TnA_GC
Cuts at: 0 824 1320
Size: 824 496
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 636 853 1320
Size: 636 217 467
Fragments arranged by size:
636 467 217
BsaI GGTCTCn'nnnn_
Cuts at: 0 1123 1320
Size: 1123 197
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 316 343 346 373 954 984 1320
Size: 316 27 3 27 581 30 336
Fragments arranged by size:
581 336 316 30 27 27 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 232 1320
Size: 232 1088
BseYI C'CCAG_C
Cuts at: 0 965 1320
Size: 965 355
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1214 1320
Size: 1214 106
BsiHKAI G_wGCw'C
Cuts at: 0 323 1320
Size: 323 997
Bsp1286I G_dGCh'C
Cuts at: 0 85 170 323 1320
Size: 85 85 153 997
Fragments arranged by size:
997 153 85 85
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 650 812 1109 1320
Size: 650 162 297 211
Fragments arranged by size:
650 297 211 162
BsrBI CCG'CTC
Cuts at: 0 1017 1170 1320
Size: 1017 153 150
Fragments arranged by size:
1017 153 150
BsrFI r'CCGG_y
Cuts at: 0 686 1320
Size: 686 634
BsrGI T'GTAC_A
Cuts at: 0 797 1320
Size: 797 523
BssHII G'CGCG_C
Cuts at: 0 829 1320
Size: 829 491
BssSI C'ACGA_G
Cuts at: 0 325 1320
Size: 325 995
BstAPI GCAn_nnn'nTGC
Cuts at: 0 513 1101 1250 1320
Size: 513 588 149 70
Fragments arranged by size:
588 513 149 70
BstEII G'GTnAC_C
Cuts at: 0 186 1320
Size: 186 1134
BstXI CCAn_nnnn'nTGG
Cuts at: 0 312 1010 1320
Size: 312 698 310
Fragments arranged by size:
698 312 310
BstYI r'GATC_y
Cuts at: 0 484 998 1320
Size: 484 514 322
Fragments arranged by size:
514 484 322
Bsu36I CC'TnA_GG
Cuts at: 0 473 818 1320
Size: 473 345 502
Fragments arranged by size:
502 473 345
BtgI C'CryG_G
Cuts at: 0 192 1320
Size: 192 1128
BtsI GCAGTG_nn'
Cuts at: 0 236 895 1163 1197 1320
Size: 236 659 268 34 123
Fragments arranged by size:
659 268 236 123 34
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 194 229 1320
Size: 194 35 1091
Fragments arranged by size:
1091 194 35
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 56 136 574 688 702 1042 1054 1148
Size: 56 80 438 114 14 340 12 94
Cuts at: 1148 1320
Size: 172
Fragments arranged by size:
438 340 172 114 94 80 56 14
12
EcoO109I rG'GnC_Cy
Cuts at: 0 201 558 981 1098 1320
Size: 201 357 423 117 222
Fragments arranged by size:
423 357 222 201 117
FspI TGC'GCA
Cuts at: 0 58 1320
Size: 58 1262
HaeII r_GCGC'y
Cuts at: 0 1212 1320
Size: 1212 108
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 586 618 851 883 1320
Size: 586 32 233 32 437
Fragments arranged by size:
586 437 233 32 32
HindIII A'AGCT_T
Cuts at: 0 871 1320
Size: 871 449
MfeI C'AATT_G
Cuts at: 0 1052 1320
Size: 1052 268
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 752 971 1320
Size: 752 219 349
Fragments arranged by size:
752 349 219
MslI CAynn'nnrTG
Cuts at: 0 310 316 1320
Size: 310 6 1004
Fragments arranged by size:
1004 310 6
MspA1I CmG'CkG
Cuts at: 0 102 909 1178 1320
Size: 102 807 269 142
Fragments arranged by size:
807 269 142 102
NaeI GCC'GGC
Cuts at: 0 688 1320
Size: 688 632
NcoI C'CATG_G
Cuts at: 0 192 1320
Size: 192 1128
NgoMIV G'CCGG_C
Cuts at: 0 686 1320
Size: 686 634
NspI r_CATG'y
Cuts at: 0 132 1078 1320
Size: 132 946 242
Fragments arranged by size:
946 242 132
PasI CC'CwG_GG
Cuts at: 0 1302 1320
Size: 1302 18
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 343 375 1320
Size: 343 32 945
Fragments arranged by size:
945 343 32
PpuMI rG'GwC_Cy
Cuts at: 0 201 981 1098 1320
Size: 201 780 117 222
Fragments arranged by size:
780 222 201 117
PshAI GACnn'nnGTC
Cuts at: 0 1288 1320
Size: 1288 32
PstI C_TGCA'G
Cuts at: 0 271 518 710 1112 1192 1246 1320
Size: 271 247 192 402 80 54 74
Fragments arranged by size:
402 271 247 192 80 74 54
PvuII CAG'CTG
Cuts at: 0 909 1320
Size: 909 411
ScaI AGT'ACT
Cuts at: 0 569 1320
Size: 569 751
SexAI A'CCwGG_T
Cuts at: 0 983 1320
Size: 983 337
SfcI C'TryA_G
Cuts at: 0 267 514 706 1108 1134 1188 1242 1320
Size: 267 247 192 402 26 54 54 78
Fragments arranged by size:
402 267 247 192 78 54 54 26
SmaI CCC'GGG
Cuts at: 0 27 1320
Size: 27 1293
SmlI C'TyrA_G
Cuts at: 0 615 868 1320
Size: 615 253 452
Fragments arranged by size:
615 452 253
StuI AGG'CCT
Cuts at: 0 331 1320
Size: 331 989
StyI C'CwwG_G
Cuts at: 0 192 854 1320
Size: 192 662 466
Fragments arranged by size:
662 466 192
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 205 1320
Size: 205 1115
TatI w'GTAC_w
Cuts at: 0 567 797 1320
Size: 567 230 523
Fragments arranged by size:
567 523 230
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 316 348 1320
Size: 316 32 972
Fragments arranged by size:
972 316 32
Tth111I GACn'n_nGTC
Cuts at: 0 397 1320
Size: 397 923
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 312 457 1320
Size: 312 145 863
Fragments arranged by size:
863 312 145
XmaI C'CCGG_G
Cuts at: 0 25 1320
Size: 25 1295
XmnI GAAnn'nnTTC
Cuts at: 0 253 298 1320
Size: 253 45 1022
Fragments arranged by size:
1022 253 45
Enzymes that do cut:
AccI AcuI AfeI AlfI AloI AlwNI ApaLI ApoI
AvaI BamHI BanII BbsI BlpI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspMI BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI CspCI
Eco57MI EcoO109I FspI HaeII Hin4I HindIII MfeI MmeI
MslI MspA1I NaeI NcoI NgoMIV NspI PasI PpiI
PpuMI PshAI PstI PvuII ScaI SexAI SfcI SmaI
SmlI StuI StyI TaqII TatI TstI Tth111I XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AflIII AgeI AhdI
AleI ApaI AscI AseI AsiSI AvrII BaeI BanI
BbeI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BmgBI BmtI BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrDI BstBI BstZ17I BtgZI
ClaI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspAI HincII
HpaI KasI KpnI MluI MscI NarI NdeI NheI
NotI NruI NsiI PacI PciI PflMI PfoI PmeI
PmlI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI SwaI XbaI
XhoI ZraI