Restriction map for
pF1KB8333
(Linear) (Six-base) MAPSORT of: KIBB8333.seq Check: 8553 from: 1 to: 939
KIBB8333.orf
With 182 enzymes: SgfI *
March 3, 2008 11:05 ..
Acc65I G'GTAC_C
Cuts at: 0 414 939
Size: 414 525
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 622 939
Size: 622 317
AflIII A'CryG_T
Cuts at: 0 849 851 939
Size: 849 2 88
Fragments arranged by size:
849 88 2
AhdI GACnn_n'nnGTC
Cuts at: 0 825 939
Size: 825 114
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 88 939
Size: 88 851
AvaI C'yCGr_G
Cuts at: 0 160 939
Size: 160 779
BamHI G'GATC_C
Cuts at: 0 636 939
Size: 636 303
BanI G'GyrC_C
Cuts at: 0 40 91 414 545 939
Size: 40 51 323 131 394
Fragments arranged by size:
394 323 131 51 40
BanII G_rGCy'C
Cuts at: 0 434 838 939
Size: 434 404 101
Fragments arranged by size:
434 404 101
BbeI G_GCGC'C
Cuts at: 0 95 549 939
Size: 95 454 390
Fragments arranged by size:
454 390 95
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 217 251 939
Size: 217 34 688
Fragments arranged by size:
688 217 34
Bme1580I G_kGCm'C
Cuts at: 0 405 534 905 939
Size: 405 129 371 34
Fragments arranged by size:
405 371 129 34
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 30 939
Size: 30 909
Bpu10I CC'TnA_GC
Cuts at: 0 143 736 939
Size: 143 593 203
Fragments arranged by size:
593 203 143
BsaAI yAC'GTr
Cuts at: 0 852 939
Size: 852 87
BsaHI Gr'CG_yC
Cuts at: 0 92 252 546 939
Size: 92 160 294 393
Fragments arranged by size:
393 294 160 92
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 871 901 939
Size: 871 30 38
Fragments arranged by size:
871 38 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 438 637 939
Size: 438 199 302
Fragments arranged by size:
438 302 199
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 607 939
Size: 607 332
BsiEI CG_ry'CG
Cuts at: 0 807 939
Size: 807 132
BsiHKAI G_wGCw'C
Cuts at: 0 838 939
Size: 838 101
BsmI GAATG_Cn'
Cuts at: 0 319 939
Size: 319 620
Bsp1286I G_dGCh'C
Cuts at: 0 405 434 534 838 905 939
Size: 405 29 100 304 67 34
Fragments arranged by size:
405 304 100 67 34 29
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 890 939
Size: 890 49
BsrDI GCAATG_nn'
Cuts at: 0 65 939
Size: 65 874
BssSI C'ACGA_G
Cuts at: 0 535 939
Size: 535 404
BstYI r'GATC_y
Cuts at: 0 636 723 939
Size: 636 87 216
Fragments arranged by size:
636 216 87
BtgI C'CryG_G
Cuts at: 0 60 549 939
Size: 60 489 390
Fragments arranged by size:
489 390 60
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 367 402 939
Size: 367 35 537
Fragments arranged by size:
537 367 35
EaeI y'GGCC_r
Cuts at: 0 18 57 111 423 574 804 939
Size: 18 39 54 312 151 230 135
Fragments arranged by size:
312 230 151 135 54 39 18
EagI C'GGCC_G
Cuts at: 0 804 939
Size: 804 135
EcoICRI GAG'CTC
Cuts at: 0 836 939
Size: 836 103
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 30 622 939
Size: 30 592 317
Fragments arranged by size:
592 317 30
EcoO109I rG'GnC_Cy
Cuts at: 0 627 939
Size: 627 312
EcoRV GAT'ATC
Cuts at: 0 354 939
Size: 354 585
HaeII r_GCGC'y
Cuts at: 0 95 549 939
Size: 95 454 390
Fragments arranged by size:
454 390 95
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 139 171 811 843 939
Size: 139 32 640 32 96
Fragments arranged by size:
640 139 96 32 32
HincII GTy'rAC
Cuts at: 0 270 939
Size: 270 669
KasI G'GCGC_C
Cuts at: 0 91 545 939
Size: 91 454 394
Fragments arranged by size:
454 394 91
KpnI G_GTAC'C
Cuts at: 0 418 939
Size: 418 521
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 345 939
Size: 345 594
MscI TGG'CCA
Cuts at: 0 20 425 939
Size: 20 405 514
Fragments arranged by size:
514 405 20
MspA1I CmG'CkG
Cuts at: 0 421 560 939
Size: 421 139 379
Fragments arranged by size:
421 379 139
NarI GG'CG_CC
Cuts at: 0 92 546 939
Size: 92 454 393
Fragments arranged by size:
454 393 92
NcoI C'CATG_G
Cuts at: 0 549 939
Size: 549 390
NdeI CA'TA_TG
Cuts at: 0 611 939
Size: 611 328
PmlI CAC'GTG
Cuts at: 0 852 939
Size: 852 87
PpuMI rG'GwC_Cy
Cuts at: 0 627 939
Size: 627 312
PstI C_TGCA'G
Cuts at: 0 467 635 939
Size: 467 168 304
Fragments arranged by size:
467 304 168
PvuII CAG'CTG
Cuts at: 0 421 560 939
Size: 421 139 379
Fragments arranged by size:
421 379 139
SacI G_AGCT'C
Cuts at: 0 838 939
Size: 838 101
SbfI CC_TGCA'GG
Cuts at: 0 635 939
Size: 635 304
SfcI C'TryA_G
Cuts at: 0 463 631 939
Size: 463 168 308
Fragments arranged by size:
463 308 168
SfoI GGC'GCC
Cuts at: 0 93 547 939
Size: 93 454 392
Fragments arranged by size:
454 392 93
StyI C'CwwG_G
Cuts at: 0 549 939
Size: 549 390
XbaI T'CTAG_A
Cuts at: 0 255 939
Size: 255 684
XmnI GAAnn'nnTTC
Cuts at: 0 652 939
Size: 652 287
Enzymes that do cut:
Acc65I AcuI AflIII AhdI AlfI AvaI BamHI BanI
BanII BbeI BcgI Bme1580I BpmI Bpu10I BsaAI BsaHI
BsaXI BseRI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI
BsrDI BssSI BstYI BtgI CspCI EaeI EagI EcoICRI
Eco57MI EcoO109I EcoRV HaeII Hin4I HincII KasI KpnI
MmeI MscI MspA1I NarI NcoI NdeI PmlI PpuMI
PstI PvuII SacI SbfI SfcI SfoI StyI XbaI
XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AleI
AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BbsI BbvCI BciVI BclI BfrBI BglI
BglII BlpI BmgBI BmrI BmtI BplI BpuEI BsaI
BsaBI BsaWI BseYI BsiWI BsmBI BspEI BspHI BsrBI
BsrFI BsrGI BssHII BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI BtsI ClaI DraI DraIII DrdI EarI
EciI EcoNI EcoRI FalI FseI FspI FspAI HindIII
HpaI MfeI MluI MslI NaeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI ScaI SexAI SfiI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TatI TstI Tth111I XcmI
XhoI XmaI ZraI