Restriction map for
pF1KB8331
(Linear) (Six-base) MAPSORT of: KIBB8331.seq Check: 5791 from: 1 to: 630
KIBB8331.orf
With 182 enzymes: SgfI *
July 2, 2008 13:23 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 388 630
Size: 388 242
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 215 630
Size: 215 415
AvaI C'yCGr_G
Cuts at: 0 134 159 329 562 630
Size: 134 25 170 233 68
Fragments arranged by size:
233 170 134 68 25
BamHI G'GATC_C
Cuts at: 0 320 466 630
Size: 320 146 164
Fragments arranged by size:
320 164 146
BanI G'GyrC_C
Cuts at: 0 112 175 199 224 630
Size: 112 63 24 25 406
Fragments arranged by size:
406 112 63 25 24
BanII G_rGCy'C
Cuts at: 0 573 630
Size: 573 57
BbeI G_GCGC'C
Cuts at: 0 179 630
Size: 179 451
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 140 174 576 610 630
Size: 140 34 402 34 20
Fragments arranged by size:
402 140 34 34 20
BglI GCCn_nnn'nGGC
Cuts at: 0 56 83 630
Size: 56 27 547
Fragments arranged by size:
547 56 27
BglII A'GATC_T
Cuts at: 0 498 630
Size: 498 132
Bme1580I G_kGCm'C
Cuts at: 0 22 229 630
Size: 22 207 401
Fragments arranged by size:
401 207 22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 630
Size: 283 347
BsaI GGTCTCn'nnnn_
Cuts at: 0 528 630
Size: 528 102
BsaHI Gr'CG_yC
Cuts at: 0 176 630
Size: 176 454
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 185 630
Size: 185 445
BseYI C'CCAG_C
Cuts at: 0 433 462 630
Size: 433 29 168
Fragments arranged by size:
433 168 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 606 630
Size: 606 24
BsmI GAATG_Cn'
Cuts at: 0 65 630
Size: 65 565
Bsp1286I G_dGCh'C
Cuts at: 0 22 229 573 630
Size: 22 207 344 57
Fragments arranged by size:
344 207 57 22
BsrBI CCG'CTC
Cuts at: 0 345 630
Size: 345 285
BsrDI GCAATG_nn'
Cuts at: 0 93 630
Size: 93 537
BstXI CCAn_nnnn'nTGG
Cuts at: 0 580 630
Size: 580 50
BstYI r'GATC_y
Cuts at: 0 320 466 498 630
Size: 320 146 32 132
Fragments arranged by size:
320 146 132 32
BtgI C'CryG_G
Cuts at: 0 116 181 296 630
Size: 116 65 115 334
Fragments arranged by size:
334 116 115 65
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 53 630
Size: 53 577
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 453 459 630
Size: 453 6 171
Fragments arranged by size:
453 171 6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 388 630
Size: 388 242
EcoO109I rG'GnC_Cy
Cuts at: 0 58 130 155 630
Size: 58 72 25 475
Fragments arranged by size:
475 72 58 25
FspI TGC'GCA
Cuts at: 0 212 630
Size: 212 418
HaeII r_GCGC'y
Cuts at: 0 179 434 630
Size: 179 255 196
Fragments arranged by size:
255 196 179
KasI G'GCGC_C
Cuts at: 0 175 630
Size: 175 455
MslI CAynn'nnrTG
Cuts at: 0 197 277 630
Size: 197 80 353
Fragments arranged by size:
353 197 80
MspA1I CmG'CkG
Cuts at: 0 183 630
Size: 183 447
NarI GG'CG_CC
Cuts at: 0 176 630
Size: 176 454
NcoI C'CATG_G
Cuts at: 0 296 630
Size: 296 334
PasI CC'CwG_GG
Cuts at: 0 79 573 630
Size: 79 494 57
Fragments arranged by size:
494 79 57
PpuMI rG'GwC_Cy
Cuts at: 0 130 630
Size: 130 500
SacII CC_GC'GG
Cuts at: 0 184 630
Size: 184 446
SexAI A'CCwGG_T
Cuts at: 0 537 630
Size: 537 93
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 56 630
Size: 56 574
SfoI GGC'GCC
Cuts at: 0 177 630
Size: 177 453
SmaI CCC'GGG
Cuts at: 0 136 331 630
Size: 136 195 299
Fragments arranged by size:
299 195 136
SmlI C'TyrA_G
Cuts at: 0 262 630
Size: 262 368
StuI AGG'CCT
Cuts at: 0 523 630
Size: 523 107
StyI C'CwwG_G
Cuts at: 0 296 630
Size: 296 334
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 525 572 630
Size: 525 47 58
Fragments arranged by size:
525 58 47
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 208 240 630
Size: 208 32 390
Fragments arranged by size:
390 208 32
XmaI C'CCGG_G
Cuts at: 0 134 329 630
Size: 134 195 301
Fragments arranged by size:
301 195 134
XmnI GAAnn'nnTTC
Cuts at: 0 373 630
Size: 373 257
Enzymes that do cut:
AcuI AlfI AvaI BamHI BanI BanII BbeI BcgI
BglI BglII Bme1580I BpuEI BsaI BsaHI BseRI BseYI
BsgI BsmI Bsp1286I BsrBI BsrDI BstXI BstYI BtgI
BtgZI EciI Eco57MI EcoO109I FspI HaeII KasI MslI
MspA1I NarI NcoI PasI PpuMI SacII SexAI SfiI
SfoI SmaI SmlI StuI StyI TaqII TstI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BbsI BbvCI BciVI
BclI BfrBI BlpI BmgBI BmrI BmtI BplI BpmI
Bpu10I BsaAI BsaBI BsaWI BsaXI BsiEI BsiHKAI BsiWI
BsmBI BspEI BspHI BspMI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstZ17I Bsu36I BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspAI Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI NaeI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SalI
SanDI SapI SbfI ScaI SfcI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI SwaI TatI Tth111I XbaI
XcmI XhoI ZraI