(Linear) (Six-base) MAPSORT of: KIBB8329.seq  Check: 7265  from: 1  to: 645



KIBB8329.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:05  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     470     645 
   Size:        470     175
 
AleI CACnn'nnGTG

Cuts at:      0     202     645 
   Size:        202     443
 
AlwNI CAG_nnn'CTG

Cuts at:      0     564     645 
   Size:        564      81
 
ApoI r'AATT_y

Cuts at:      0      50     645 
   Size:         50     595
 
AvaI C'yCGr_G

Cuts at:      0     326     645 
   Size:        326     319
 
BanI G'GyrC_C

Cuts at:      0     274     645 
   Size:        274     371
 
BglI GCCn_nnn'nGGC

Cuts at:      0      30     645 
   Size:         30     615
 
BglII A'GATC_T

Cuts at:      0     215     645 
   Size:        215     430
 
BlpI GC'TnA_GC

Cuts at:      0     252     645 
   Size:        252     393
 
Bme1580I G_kGCm'C

Cuts at:      0     279     645 
   Size:        279     366
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     171     645 
   Size:        171     474
 
BseYI C'CCAG_C

Cuts at:      0      75     229     614     645 
   Size:         75     154     385      31

  Fragments arranged by size: 

                 385     154      75      31
 
BsiHKAI G_wGCw'C

Cuts at:      0     154     645 
   Size:        154     491
 
Bsp1286I G_dGCh'C

Cuts at:      0     154     279     645 
   Size:        154     125     366

  Fragments arranged by size: 

                 366     154     125
 
BsrBI CCG'CTC

Cuts at:      0     163     645 
   Size:        163     482
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      53     645 
   Size:         53     592
 
BstYI r'GATC_y

Cuts at:      0     215     597     645 
   Size:        215     382      48

  Fragments arranged by size: 

                 382     215      48
 
BtsI GCAGTG_nn'

Cuts at:      0      95     645 
   Size:         95     550
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     365     645 
   Size:        365     280
 
EaeI y'GGCC_r

Cuts at:      0     410     645 
   Size:        410     235
 
EarI CTCTTCn'nnn_

Cuts at:      0     431     523     645 
   Size:        431      92     122

  Fragments arranged by size: 

                 431     122      92
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     470     645 
   Size:        470     175
 
EcoRV GAT'ATC

Cuts at:      0     244     645 
   Size:        244     401
 
MscI TGG'CCA

Cuts at:      0     412     645 
   Size:        412     233
 
MslI CAynn'nnrTG

Cuts at:      0     202     645 
   Size:        202     443
 
MspA1I CmG'CkG

Cuts at:      0     229     645 
   Size:        229     416
 
NdeI CA'TA_TG

Cuts at:      0     450     645 
   Size:        450     195
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     564     645 
   Size:        564      81
 
PstI C_TGCA'G

Cuts at:      0      90     645 
   Size:         90     555
 
PvuII CAG'CTG

Cuts at:      0     229     645 
   Size:        229     416
 
SfcI C'TryA_G

Cuts at:      0      86     267     352     645 
   Size:         86     181      85     293

  Fragments arranged by size: 

                 293     181      86      85
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     190     330     645 
   Size:        190     140     315

  Fragments arranged by size: 

                 315     190     140
 
TatI w'GTAC_w

Cuts at:      0     263     645 
   Size:        263     382
 
XbaI T'CTAG_A

Cuts at:      0     189     645 
   Size:        189     456
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     420     645 
   Size:        420     225
 

 Enzymes that do cut:

     AcuI     AleI    AlwNI     ApoI     AvaI     BanI     BglI    BglII
     BlpI Bme1580I     BmrI    BseYI  BsiHKAI Bsp1286I    BsrBI   BstAPI
    BstYI     BtsI     DrdI     EaeI     EarI  Eco57MI    EcoRV     MscI
     MslI   MspA1I     NdeI    PflMI     PstI    PvuII     SfcI    TaqII
     TatI     XbaI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI    BmgBI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI
   DraIII     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI     FalI
     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     NaeI     NarI     NcoI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI     TstI  Tth111I     XhoI     XmaI     XmnI
     ZraI