Restriction map for
pF1KB8326
(Linear) (Six-base) MAPSORT of: KIBB8326.seq Check: 8999 from: 1 to: 1098
KIBB8326.orf
With 182 enzymes: SgfI *
March 3, 2008 11:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 243 587 1098
Size: 243 344 511
Fragments arranged by size:
511 344 243
AlwNI CAG_nnn'CTG
Cuts at: 0 18 171 351 1098
Size: 18 153 180 747
Fragments arranged by size:
747 180 153 18
ApaI G_GGCC'C
Cuts at: 0 669 1098
Size: 669 429
ApaLI G'TGCA_C
Cuts at: 0 574 1098
Size: 574 524
AvaI C'yCGr_G
Cuts at: 0 565 1098
Size: 565 533
BanI G'GyrC_C
Cuts at: 0 328 490 588 912 1098
Size: 328 162 98 324 186
Fragments arranged by size:
328 324 186 162 98
BanII G_rGCy'C
Cuts at: 0 669 905 980 1091 1098
Size: 669 236 75 111 7
Fragments arranged by size:
669 236 111 75 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 617 1098
Size: 617 481
BbvCI CC'TCA_GC
Cuts at: 0 466 1098
Size: 466 632
BfrBI ATG'CAT
Cuts at: 0 297 1098
Size: 297 801
BglI GCCn_nnn'nGGC
Cuts at: 0 181 681 1098
Size: 181 500 417
Fragments arranged by size:
500 417 181
Bme1580I G_kGCm'C
Cuts at: 0 75 212 578 593 669 997 1098
Size: 75 137 366 15 76 328 101
Fragments arranged by size:
366 328 137 101 76 75 15
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 680 796 1098
Size: 160 520 116 302
Fragments arranged by size:
520 302 160 116
Bpu10I CC'TnA_GC
Cuts at: 0 333 466 1098
Size: 333 133 632
Fragments arranged by size:
632 333 133
BsaI GGTCTCn'nnnn_
Cuts at: 0 139 408 543 1098
Size: 139 269 135 555
Fragments arranged by size:
555 269 139 135
BsaHI Gr'CG_yC
Cuts at: 0 1043 1098
Size: 1043 55
BsaWI w'CCGG_w
Cuts at: 0 798 1098
Size: 798 300
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 769 799 1098
Size: 769 30 299
Fragments arranged by size:
769 299 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 157 606 722 793 824 827 1098
Size: 157 449 116 71 31 3 271
Fragments arranged by size:
449 271 157 116 71 31 3
BseYI C'CCAG_C
Cuts at: 0 276 712 904 908 1090 1098
Size: 276 436 192 4 182 8
Fragments arranged by size:
436 276 192 182 8 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 1098
Size: 133 965
BsiEI CG_ry'CG
Cuts at: 0 641 1098
Size: 641 457
BsiHKAI G_wGCw'C
Cuts at: 0 578 980 1098
Size: 578 402 118
Fragments arranged by size:
578 402 118
Bsp1286I G_dGCh'C
Cuts at: 0 75 212 578 593 669 905 980 997
Size: 75 137 366 15 76 236 75 17
Cuts at: 997 1091 1098
Size: 94 7
Fragments arranged by size:
366 236 137 94 76 75 75 17
15 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 239 243 587 836 1098
Size: 239 4 344 249 262
Fragments arranged by size:
344 262 249 239 4
BsrFI r'CCGG_y
Cuts at: 0 582 640 1098
Size: 582 58 458
Fragments arranged by size:
582 458 58
BstAPI GCAn_nnn'nTGC
Cuts at: 0 216 1098
Size: 216 882
BstXI CCAn_nnnn'nTGG
Cuts at: 0 720 1098
Size: 720 378
BstYI r'GATC_y
Cuts at: 0 32 1098
Size: 32 1066
BtgI C'CryG_G
Cuts at: 0 149 450 1098
Size: 149 301 648
Fragments arranged by size:
648 301 149
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 357 1098
Size: 357 741
EaeI y'GGCC_r
Cuts at: 0 42 173 203 360 447 638 1098
Size: 42 131 30 157 87 191 460
Fragments arranged by size:
460 191 157 131 87 42 30
EagI C'GGCC_G
Cuts at: 0 638 1098
Size: 638 460
EarI CTCTTCn'nnn_
Cuts at: 0 800 1098
Size: 800 298
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 525 597 1098
Size: 19 506 72 501
Fragments arranged by size:
506 501 72 19
EcoICRI GAG'CTC
Cuts at: 0 978 1098
Size: 978 120
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 680 796 1098
Size: 160 520 116 302
Fragments arranged by size:
520 302 160 116
EcoNI CCTnn'n_nnAGG
Cuts at: 0 250 408 1098
Size: 250 158 690
Fragments arranged by size:
690 250 158
EcoO109I rG'GnC_Cy
Cuts at: 0 462 516 665 666 1098
Size: 462 54 149 1 432
Fragments arranged by size:
462 432 149 54 1
FspI TGC'GCA
Cuts at: 0 220 1098
Size: 220 878
HaeII r_GCGC'y
Cuts at: 0 139 650 1098
Size: 139 511 448
Fragments arranged by size:
511 448 139
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 769 801 1098
Size: 769 32 297
Fragments arranged by size:
769 297 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 206 1098
Size: 206 892
MscI TGG'CCA
Cuts at: 0 175 449 1098
Size: 175 274 649
Fragments arranged by size:
649 274 175
MslI CAynn'nnrTG
Cuts at: 0 298 1098
Size: 298 800
MspA1I CmG'CkG
Cuts at: 0 18 348 477 762 908 1094 1098
Size: 18 330 129 285 146 186 4
Fragments arranged by size:
330 285 186 146 129 18 4
NaeI GCC'GGC
Cuts at: 0 642 1098
Size: 642 456
NcoI C'CATG_G
Cuts at: 0 149 450 1098
Size: 149 301 648
Fragments arranged by size:
648 301 149
NdeI CA'TA_TG
Cuts at: 0 299 1098
Size: 299 799
NgoMIV G'CCGG_C
Cuts at: 0 640 1098
Size: 640 458
NsiI A_TGCA'T
Cuts at: 0 299 1098
Size: 299 799
NspI r_CATG'y
Cuts at: 0 225 297 1098
Size: 225 72 801
Fragments arranged by size:
801 225 72
PasI CC'CwG_GG
Cuts at: 0 1072 1098
Size: 1072 26
PflMI CCAn_nnn'nTGG
Cuts at: 0 171 889 1098
Size: 171 718 209
Fragments arranged by size:
718 209 171
PpuMI rG'GwC_Cy
Cuts at: 0 462 1098
Size: 462 636
PspOMI G'GGCC_C
Cuts at: 0 665 1098
Size: 665 433
PstI C_TGCA'G
Cuts at: 0 23 236 412 1098
Size: 23 213 176 686
Fragments arranged by size:
686 213 176 23
PvuII CAG'CTG
Cuts at: 0 18 477 908 1094 1098
Size: 18 459 431 186 4
Fragments arranged by size:
459 431 186 18 4
SacI G_AGCT'C
Cuts at: 0 980 1098
Size: 980 118
SapI GCTCTTCn'nnn_
Cuts at: 0 800 1098
Size: 800 298
SbfI CC_TGCA'GG
Cuts at: 0 236 1098
Size: 236 862
SexAI A'CCwGG_T
Cuts at: 0 188 1098
Size: 188 910
SfcI C'TryA_G
Cuts at: 0 10 19 232 408 1098
Size: 10 9 213 176 690
Fragments arranged by size:
690 213 176 10 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 181 1098
Size: 181 917
StyI C'CwwG_G
Cuts at: 0 149 450 542 677 719 1098
Size: 149 301 92 135 42 379
Fragments arranged by size:
379 301 149 135 92 42
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 900 1098
Size: 900 198
Enzymes that do cut:
AarI AlwNI ApaI ApaLI AvaI BanI BanII BbsI
BbvCI BfrBI BglI Bme1580I BpmI Bpu10I BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspMI BsrFI BstAPI BstXI BstYI BtgI BtgZI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI
HaeII Hin4I MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NsiI NspI PasI PflMI PpuMI PspOMI
PstI PvuII SacI SapI SbfI SexAI SfcI SfiI
StyI TaqII
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BcgI BciVI BclI
BglII BlpI BmgBI BmrI BmtI BplI BpuEI BsaAI
BsaBI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI
BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtsI
ClaI CspCI DraI DraIII DrdI EcoRI EcoRV FalI
FseI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI NarI NheI NotI NruI PacI PciI PfoI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SalI SanDI ScaI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI