(Linear) (Six-base) MAPSORT of: KIBB8325.seq  Check: 7422  from: 1  to: 618



KIBB8325.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:05  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      46     618 
   Size:         46     572
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     204     236     618 
   Size:        204      32     382

  Fragments arranged by size: 

                 382     204      32
 
ApaLI G'TGCA_C

Cuts at:      0     239     618 
   Size:        239     379
 
ApoI r'AATT_y

Cuts at:      0     412     618 
   Size:        412     206
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     272     305     618 
   Size:        272      33     313

  Fragments arranged by size: 

                 313     272      33
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     180     618 
   Size:        180     438
 
Bme1580I G_kGCm'C

Cuts at:      0     243     618 
   Size:        243     375
 
BmgBI CAC'GTC

Cuts at:      0      12     618 
   Size:         12     606
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      96     618 
   Size:         96     522
 
BsaBI GATnn'nnATC

Cuts at:      0      34     618 
   Size:         34     584
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     204     234     618 
   Size:        204      30     384

  Fragments arranged by size: 

                 384     204      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     599     602     618 
   Size:        599       3      16

  Fragments arranged by size: 

                 599      16       3
 
BseYI C'CCAG_C

Cuts at:      0     223     618 
   Size:        223     395
 
BsiHKAI G_wGCw'C

Cuts at:      0     243     618 
   Size:        243     375
 
BsmI GAATG_Cn'

Cuts at:      0     426     618 
   Size:        426     192
 
Bsp1286I G_dGCh'C

Cuts at:      0     243     618 
   Size:        243     375
 
BsrGI T'GTAC_A

Cuts at:      0     328     471     618 
   Size:        328     143     147

  Fragments arranged by size: 

                 328     147     143
 
BstYI r'GATC_y

Cuts at:      0      29     618 
   Size:         29     589
 
ClaI AT'CG_AT

Cuts at:      0     371     618 
   Size:        371     247
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     134     169     618 
   Size:        134      35     449

  Fragments arranged by size: 

                 449     134      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     247     618 
   Size:        247     371
 
EarI CTCTTCn'nnn_

Cuts at:      0     270     618 
   Size:        270     348
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      46     618 
   Size:         46     572
 
HincII GTy'rAC

Cuts at:      0     129     618 
   Size:        129     489
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     339     618 
   Size:        339     279
 
NspI r_CATG'y

Cuts at:      0     354     618 
   Size:        354     264
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     204     236     618 
   Size:        204      32     382

  Fragments arranged by size: 

                 382     204      32
 
SmlI C'TyrA_G

Cuts at:      0      75     618 
   Size:         75     543
 
StuI AGG'CCT

Cuts at:      0     407     618 
   Size:        407     211
 
StyI C'CwwG_G

Cuts at:      0     579     618 
   Size:        579      39
 
TatI w'GTAC_w

Cuts at:      0     328     471     618 
   Size:        328     143     147

  Fragments arranged by size: 

                 328     147     143
 
XmnI GAAnn'nnTTC

Cuts at:      0     426     618 
   Size:        426     192
 

 Enzymes that do cut:

     AcuI     AloI    ApaLI     ApoI     BaeI    BciVI Bme1580I    BmgBI
    BpuEI    BsaBI    BsaXI    BseRI    BseYI  BsiHKAI     BsmI Bsp1286I
    BsrGI    BstYI     ClaI    CspCI   DraIII     EarI  Eco57MI   HincII
     MmeI     NspI     PpiI     SmlI     StuI     StyI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI     AscI     AseI
    AsiSI     AvaI    AvrII    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI     BclI    BfrBI     BglI    BglII     BlpI     BmrI
     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaHI    BsaWI
     BsgI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI
     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII    Hin4I  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI    TaqII     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     ZraI