Restriction map for
pF1KB8321
(Linear) (Six-base) MAPSORT of: KIBB8321.seq Check: 8673 from: 1 to: 876
KIBB8321.orf
With 182 enzymes: SgfI *
March 3, 2008 11:04 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 610 876
Size: 610 266
AflII C'TTAA_G
Cuts at: 0 613 876
Size: 613 263
AflIII A'CryG_T
Cuts at: 0 317 876
Size: 317 559
AleI CACnn'nnGTG
Cuts at: 0 793 876
Size: 793 83
AlwNI CAG_nnn'CTG
Cuts at: 0 192 876
Size: 192 684
ApoI r'AATT_y
Cuts at: 0 124 157 298 538 876
Size: 124 33 141 240 338
Fragments arranged by size:
338 240 141 124 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 616 876
Size: 616 260
BciVI GTATCCnnnnn_n'
Cuts at: 0 272 876
Size: 272 604
BclI T'GATC_A
Cuts at: 0 639 876
Size: 639 237
BglII A'GATC_T
Cuts at: 0 339 876
Size: 339 537
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 454 876
Size: 454 422
Bpu10I CC'TnA_GC
Cuts at: 0 783 876
Size: 783 93
BsaBI GATnn'nnATC
Cuts at: 0 844 876
Size: 844 32
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 307 337 876
Size: 307 30 539
Fragments arranged by size:
539 307 30
BseYI C'CCAG_C
Cuts at: 0 228 876
Size: 228 648
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 385 876
Size: 385 491
BsiHKAI G_wGCw'C
Cuts at: 0 95 876
Size: 95 781
Bsp1286I G_dGCh'C
Cuts at: 0 95 876
Size: 95 781
BsrDI GCAATG_nn'
Cuts at: 0 207 876
Size: 207 669
BstYI r'GATC_y
Cuts at: 0 77 339 876
Size: 77 262 537
Fragments arranged by size:
537 262 77
BtgI C'CryG_G
Cuts at: 0 113 876
Size: 113 763
BtsI GCAGTG_nn'
Cuts at: 0 397 691 876
Size: 397 294 185
Fragments arranged by size:
397 294 185
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 747 782 876
Size: 747 35 94
Fragments arranged by size:
747 94 35
DraI TTT'AAA
Cuts at: 0 215 876
Size: 215 661
EarI CTCTTCn'nnn_
Cuts at: 0 59 374 521 592 876
Size: 59 315 147 71 284
Fragments arranged by size:
315 284 147 71 59
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 610 876
Size: 610 266
EcoNI CCTnn'n_nnAGG
Cuts at: 0 310 876
Size: 310 566
EcoRI G'AATT_C
Cuts at: 0 538 876
Size: 538 338
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 307 339 808 840 876
Size: 307 32 469 32 36
Fragments arranged by size:
469 307 36 32 32
HincII GTy'rAC
Cuts at: 0 207 876
Size: 207 669
MfeI C'AATT_G
Cuts at: 0 149 876
Size: 149 727
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 626 876
Size: 626 250
MslI CAynn'nnrTG
Cuts at: 0 793 876
Size: 793 83
MspA1I CmG'CkG
Cuts at: 0 228 876
Size: 228 648
NcoI C'CATG_G
Cuts at: 0 113 876
Size: 113 763
NspI r_CATG'y
Cuts at: 0 321 870 876
Size: 321 549 6
Fragments arranged by size:
549 321 6
PciI A'CATG_T
Cuts at: 0 317 876
Size: 317 559
PstI C_TGCA'G
Cuts at: 0 9 876
Size: 9 867
PvuII CAG'CTG
Cuts at: 0 228 876
Size: 228 648
SapI GCTCTTCn'nnn_
Cuts at: 0 592 876
Size: 592 284
ScaI AGT'ACT
Cuts at: 0 763 876
Size: 763 113
SfcI C'TryA_G
Cuts at: 0 5 361 876
Size: 5 356 515
Fragments arranged by size:
515 356 5
SmlI C'TyrA_G
Cuts at: 0 613 876
Size: 613 263
StyI C'CwwG_G
Cuts at: 0 113 876
Size: 113 763
TatI w'GTAC_w
Cuts at: 0 320 761 876
Size: 320 441 115
Fragments arranged by size:
441 320 115
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 111 143 876
Size: 111 32 733
Fragments arranged by size:
733 111 32
Tth111I GACn'n_nGTC
Cuts at: 0 448 876
Size: 448 428
XbaI T'CTAG_A
Cuts at: 0 57 876
Size: 57 819
XmnI GAAnn'nnTTC
Cuts at: 0 542 559 876
Size: 542 17 317
Fragments arranged by size:
542 317 17
Enzymes that do cut:
AcuI AflII AflIII AleI AlwNI ApoI BbsI BciVI
BclI BglII BplI Bpu10I BsaBI BsaXI BseYI BsgI
BsiHKAI Bsp1286I BsrDI BstYI BtgI BtsI CspCI DraI
EarI Eco57MI EcoNI EcoRI Hin4I HincII MfeI MmeI
MslI MspA1I NcoI NspI PciI PstI PvuII SapI
ScaI SfcI SmlI StyI TatI TstI Tth111I XbaI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BanII BbeI BbvCI BcgI
BfrBI BglI BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaHI BsaWI BseRI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI ClaI DraIII DrdI EaeI EagI EciI EcoICRI
EcoO109I EcoRV FalI FseI FspI FspAI HaeII HindIII
HpaI KasI KpnI MluI MscI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII XcmI
XhoI XmaI ZraI