(Linear) (Six-base) MAPSORT of: KIBB8320.seq  Check: 3219  from: 1  to: 387



KIBB8320.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:04  ..

ApoI r'AATT_y

Cuts at:      0     193     212     344     387 
   Size:        193      19     132      43

  Fragments arranged by size: 

                 193     132      43      19
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     341     387 
   Size:        341      46
 
BsiHKAI G_wGCw'C

Cuts at:      0     355     387 
   Size:        355      32
 
BsmI GAATG_Cn'

Cuts at:      0     246     387 
   Size:        246     141
 
Bsp1286I G_dGCh'C

Cuts at:      0     355     387 
   Size:        355      32
 
BspHI T'CATG_A

Cuts at:      0     266     387 
   Size:        266     121
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     116     151     387 
   Size:        116      35     236

  Fragments arranged by size: 

                 236     116      35
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     341     387 
   Size:        341      46
 
EcoO109I rG'GnC_Cy

Cuts at:      0     301     387 
   Size:        301      86
 
EcoRI G'AATT_C

Cuts at:      0     212     387 
   Size:        212     175
 
HindIII A'AGCT_T

Cuts at:      0     181     387 
   Size:        181     206
 
MfeI C'AATT_G

Cuts at:      0     128     387 
   Size:        128     259
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      45     387 
   Size:         45     342
 
MslI CAynn'nnrTG

Cuts at:      0     271     387 
   Size:        271     116
 
MspA1I CmG'CkG

Cuts at:      0     280     316     387 
   Size:        280      36      71

  Fragments arranged by size: 

                 280      71      36
 
PfoI T'CCnGG_A

Cuts at:      0     303     387 
   Size:        303      84
 
PpuMI rG'GwC_Cy

Cuts at:      0     301     387 
   Size:        301      86
 
PvuII CAG'CTG

Cuts at:      0     280     316     387 
   Size:        280      36      71

  Fragments arranged by size: 

                 280      71      36
 
SanDI GG'GwC_CC

Cuts at:      0     301     387 
   Size:        301      86
 
SfcI C'TryA_G

Cuts at:      0     155     387 
   Size:        155     232
 
StyI C'CwwG_G

Cuts at:      0     207     387 
   Size:        207     180
 

 Enzymes that do cut:

     ApoI     BpmI  BsiHKAI     BsmI Bsp1286I    BspHI    CspCI  Eco57MI
 EcoO109I    EcoRI  HindIII     MfeI     MmeI     MslI   MspA1I     PfoI
    PpuMI    PvuII    SanDI     SfcI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsiWI    BsmBI    BspEI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI    EcoNI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII     HpaI     KasI     KpnI     MluI     MscI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI