(Linear) (Six-base) MAPSORT of: KIBB8318.seq  Check: 8727  from: 1  to: 741



KIBB8318.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:04  ..

Acc65I G'GTAC_C

Cuts at:      0     199     293     741 
   Size:        199      94     448

  Fragments arranged by size: 

                 448     199      94
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     194     710     741 
   Size:        194     516      31

  Fragments arranged by size: 

                 516     194      31
 
AflIII A'CryG_T

Cuts at:      0     680     682     741 
   Size:        680       2      59

  Fragments arranged by size: 

                 680      59       2
 
AleI CACnn'nnGTG

Cuts at:      0      25     741 
   Size:         25     716
 
ApoI r'AATT_y

Cuts at:      0     136     399     424     622     670     741 
   Size:        136     263      25     198      48      71

  Fragments arranged by size: 

                 263     198     136      71      48      25
 
BanI G'GyrC_C

Cuts at:      0     199     293     741 
   Size:        199      94     448

  Fragments arranged by size: 

                 448     199      94
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     516     698     741 
   Size:        516     182      43

  Fragments arranged by size: 

                 516     182      43
 
BbvCI CC'TCA_GC

Cuts at:      0     488     741 
   Size:        488     253
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     493     741 
   Size:        493     248
 
Bpu10I CC'TnA_GC

Cuts at:      0     488     741 
   Size:        488     253
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       8     741 
   Size:          8     733
 
BsaAI yAC'GTr

Cuts at:      0     683     741 
   Size:        683      58
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     192     222     267     297     741 
   Size:        192      30      45      30     444

  Fragments arranged by size: 

                 444     192      45      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     274     275     741 
   Size:        274       1     466

  Fragments arranged by size: 

                 466     274       1
 
BspHI T'CATG_A

Cuts at:      0     110     741 
   Size:        110     631
 
EarI CTCTTCn'nnn_

Cuts at:      0     730     741 
   Size:        730      11
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     194     710     741 
   Size:        194     516      31

  Fragments arranged by size: 

                 516     194      31
 
EcoRI G'AATT_C

Cuts at:      0     424     622     741 
   Size:        424     198     119

  Fragments arranged by size: 

                 424     198     119
 
HincII GTy'rAC

Cuts at:      0     156     741 
   Size:        156     585
 
KpnI G_GTAC'C

Cuts at:      0     203     297     741 
   Size:        203      94     444

  Fragments arranged by size: 

                 444     203      94
 
MslI CAynn'nnrTG

Cuts at:      0      25     741 
   Size:         25     716
 
PmlI CAC'GTG

Cuts at:      0     683     741 
   Size:        683      58
 
SmlI C'TyrA_G

Cuts at:      0      23     741 
   Size:         23     718
 
StuI AGG'CCT

Cuts at:      0       8     741 
   Size:          8     733
 
StyI C'CwwG_G

Cuts at:      0     289     741 
   Size:        289     452
 
TatI w'GTAC_w

Cuts at:      0     314     741 
   Size:        314     427
 

 Enzymes that do cut:

   Acc65I     AcuI   AflIII     AleI     ApoI     BanI     BbsI    BbvCI
     BmrI   Bpu10I    BpuEI    BsaAI    BsaXI    BseRI    BspHI     EarI
  Eco57MI    EcoRI   HincII     KpnI     MslI     PmlI     SmlI     StuI
     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII     AgeI     AhdI
     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI    BanII     BbeI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI
     BplI     BpmI     BsaI    BsaBI    BsaHI    BsaWI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I  HindIII     HpaI     KasI     MfeI     MluI     MmeI
     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI