Restriction map for
pF1KB8317
(Linear) (Six-base) MAPSORT of: KIBB8317.seq Check: 8194 from: 1 to: 1458
KIBB8317.orf
With 182 enzymes: SgfI *
March 3, 2008 11:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 612 1458
Size: 612 846
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 323 1027 1458
Size: 323 704 431
Fragments arranged by size:
704 431 323
AflII C'TTAA_G
Cuts at: 0 322 1458
Size: 322 1136
AlwNI CAG_nnn'CTG
Cuts at: 0 550 835 1235 1458
Size: 550 285 400 223
Fragments arranged by size:
550 400 285 223
AvaI C'yCGr_G
Cuts at: 0 923 1177 1458
Size: 923 254 281
Fragments arranged by size:
923 281 254
BanI G'GyrC_C
Cuts at: 0 583 1458
Size: 583 875
BanII G_rGCy'C
Cuts at: 0 23 134 167 493 1024 1178 1186 1214
Size: 23 111 33 326 531 154 8 28
Cuts at: 1214 1458
Size: 244
Fragments arranged by size:
531 326 244 154 111 33 28 23
8
BbsI GAAGACnn'nnnn_
Cuts at: 0 47 863 1015 1458
Size: 47 816 152 443
Fragments arranged by size:
816 443 152 47
BbvCI CC'TCA_GC
Cuts at: 0 701 770 1458
Size: 701 69 688
Fragments arranged by size:
701 688 69
BclI T'GATC_A
Cuts at: 0 371 740 1458
Size: 371 369 718
Fragments arranged by size:
718 371 369
BglI GCCn_nnn'nGGC
Cuts at: 0 844 1333 1458
Size: 844 489 125
Fragments arranged by size:
844 489 125
BlpI GC'TnA_GC
Cuts at: 0 161 1458
Size: 161 1297
BmgBI CAC'GTC
Cuts at: 0 608 1458
Size: 608 850
BmrI ACTGGGnnnn_n'
Cuts at: 0 488 1458
Size: 488 970
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 527 658 1291 1458
Size: 37 490 131 633 167
Fragments arranged by size:
633 490 167 131 37
Bpu10I CC'TnA_GC
Cuts at: 0 22 701 770 1172 1208 1458
Size: 22 679 69 402 36 250
Fragments arranged by size:
679 402 250 69 36 22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 61 1242 1458
Size: 46 15 1181 216
Fragments arranged by size:
1181 216 46 15
BsaI GGTCTCn'nnnn_
Cuts at: 0 585 886 1458
Size: 585 301 572
Fragments arranged by size:
585 572 301
BsaHI Gr'CG_yC
Cuts at: 0 567 1435 1458
Size: 567 868 23
Fragments arranged by size:
868 567 23
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1065 1258 1274 1429 1458
Size: 1065 193 16 155 29
Fragments arranged by size:
1065 193 155 29 16
BseYI C'CCAG_C
Cuts at: 0 7 349 1458
Size: 7 342 1109
Fragments arranged by size:
1109 342 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 640 907 1458
Size: 157 483 267 551
Fragments arranged by size:
551 483 267 157
BsiEI CG_ry'CG
Cuts at: 0 1337 1458
Size: 1337 121
BsiHKAI G_wGCw'C
Cuts at: 0 1097 1186 1458
Size: 1097 89 272
Fragments arranged by size:
1097 272 89
BsmBI CGTCTCn'nnnn_
Cuts at: 0 922 1458
Size: 922 536
Bsp1286I G_dGCh'C
Cuts at: 0 23 134 167 493 1024 1097 1178 1186
Size: 23 111 33 326 531 73 81 8
Cuts at: 1186 1214 1458
Size: 28 244
Fragments arranged by size:
531 326 244 111 81 73 33 28
23 8
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 612 1458
Size: 612 846
BsrBI CCG'CTC
Cuts at: 0 344 581 995 1458
Size: 344 237 414 463
Fragments arranged by size:
463 414 344 237
BssSI C'ACGA_G
Cuts at: 0 588 1458
Size: 588 870
BstAPI GCAn_nnn'nTGC
Cuts at: 0 835 1235 1458
Size: 835 400 223
Fragments arranged by size:
835 400 223
BstBI TT'CG_AA
Cuts at: 0 425 1458
Size: 425 1033
Bsu36I CC'TnA_GG
Cuts at: 0 782 1458
Size: 782 676
BtgI C'CryG_G
Cuts at: 0 1313 1331 1458
Size: 1313 18 127
Fragments arranged by size:
1313 127 18
BtsI GCAGTG_nn'
Cuts at: 0 1141 1458
Size: 1141 317
DraI TTT'AAA
Cuts at: 0 84 1458
Size: 84 1374
EaeI y'GGCC_r
Cuts at: 0 339 602 1334 1458
Size: 339 263 732 124
Fragments arranged by size:
732 339 263 124
EagI C'GGCC_G
Cuts at: 0 1334 1458
Size: 1334 124
EarI CTCTTCn'nnn_
Cuts at: 0 104 985 1458
Size: 104 881 473
Fragments arranged by size:
881 473 104
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 198 1044 1458
Size: 198 846 414
Fragments arranged by size:
846 414 198
EcoICRI GAG'CTC
Cuts at: 0 1184 1458
Size: 1184 274
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 323 527 658 1027 1291 1458
Size: 37 286 204 131 369 264 167
Fragments arranged by size:
369 286 264 204 167 131 37
EcoO109I rG'GnC_Cy
Cuts at: 0 48 57 274 633 1131 1191 1458
Size: 48 9 217 359 498 60 267
Fragments arranged by size:
498 359 267 217 60 48 9
HaeII r_GCGC'y
Cuts at: 0 255 1073 1458
Size: 255 818 385
Fragments arranged by size:
818 385 255
HindIII A'AGCT_T
Cuts at: 0 227 319 1458
Size: 227 92 1139
Fragments arranged by size:
1139 227 92
MspA1I CmG'CkG
Cuts at: 0 249 835 1315 1458
Size: 249 586 480 143
Fragments arranged by size:
586 480 249 143
PasI CC'CwG_GG
Cuts at: 0 381 1024 1458
Size: 381 643 434
Fragments arranged by size:
643 434 381
PflMI CCAn_nnn'nTGG
Cuts at: 0 550 735 1458
Size: 550 185 723
Fragments arranged by size:
723 550 185
PfoI T'CCnGG_A
Cuts at: 0 543 635 1458
Size: 543 92 823
Fragments arranged by size:
823 543 92
PpuMI rG'GwC_Cy
Cuts at: 0 48 274 633 1191 1458
Size: 48 226 359 558 267
Fragments arranged by size:
558 359 267 226 48
SacI G_AGCT'C
Cuts at: 0 1186 1458
Size: 1186 272
SacII CC_GC'GG
Cuts at: 0 1316 1458
Size: 1316 142
SanDI GG'GwC_CC
Cuts at: 0 274 1458
Size: 274 1184
SapI GCTCTTCn'nnn_
Cuts at: 0 985 1458
Size: 985 473
SmaI CCC'GGG
Cuts at: 0 925 1458
Size: 925 533
SmlI C'TyrA_G
Cuts at: 0 61 76 322 1221 1458
Size: 61 15 246 899 237
Fragments arranged by size:
899 246 237 61 15
StyI C'CwwG_G
Cuts at: 0 596 1052 1289 1399 1458
Size: 596 456 237 110 59
Fragments arranged by size:
596 456 237 110 59
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 742 1458
Size: 742 716
TatI w'GTAC_w
Cuts at: 0 1346 1458
Size: 1346 112
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1105 1137 1458
Size: 1105 32 321
Fragments arranged by size:
1105 321 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 732 1458
Size: 732 726
XmaI C'CCGG_G
Cuts at: 0 923 1458
Size: 923 535
Enzymes that do cut:
AarI AcuI AflII AlwNI AvaI BanI BanII BbsI
BbvCI BclI BglI BlpI BmgBI BmrI BpmI Bpu10I
BpuEI BsaI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspMI BsrBI BssSI BstAPI BstBI Bsu36I
BtgI BtsI DraI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I HaeII HindIII MspA1I PasI PflMI PfoI
PpuMI SacI SacII SanDI SapI SmaI SmlI StyI
TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BcgI BciVI BfrBI
BglII Bme1580I BmtI BplI BsaAI BsaBI BsaWI BsaXI
BsiWI BsmI BspEI BspHI BsrDI BsrFI BsrGI BssHII
BstEII BstXI BstYI BstZ17I BtgZI ClaI CspCI DraIII
DrdI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
Hin4I HincII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PciI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SalI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StuI SwaI Tth111I XbaI XhoI XmnI ZraI