Restriction map for
pF1KB8310
(Linear) (Six-base) MAPSORT of: KIBB8310.seq Check: 4073 from: 1 to: 1209
KIBB8310.orf
With 182 enzymes: SgfI *
March 3, 2008 11:04 ..
AccI GT'mk_AC
Cuts at: 0 617 1209
Size: 617 592
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 649 1209
Size: 649 560
AflIII A'CryG_T
Cuts at: 0 69 1209
Size: 69 1140
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 713 1209
Size: 713 496
AlwNI CAG_nnn'CTG
Cuts at: 0 34 303 1209
Size: 34 269 906
Fragments arranged by size:
906 269 34
ApoI r'AATT_y
Cuts at: 0 153 961 1033 1155 1209
Size: 153 808 72 122 54
Fragments arranged by size:
808 153 122 72 54
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 233 266 1209
Size: 233 33 943
Fragments arranged by size:
943 233 33
BanI G'GyrC_C
Cuts at: 0 312 871 1209
Size: 312 559 338
Fragments arranged by size:
559 338 312
BbsI GAAGACnn'nnnn_
Cuts at: 0 398 1209
Size: 398 811
BfrBI ATG'CAT
Cuts at: 0 915 1209
Size: 915 294
BlpI GC'TnA_GC
Cuts at: 0 131 500 1209
Size: 131 369 709
Fragments arranged by size:
709 369 131
BmtI G_CTAG'C
Cuts at: 0 1199 1209
Size: 1199 10
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 878 1209
Size: 878 331
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 180 249 526 821 957 1209
Size: 35 145 69 277 295 136 252
Fragments arranged by size:
295 277 252 145 136 69 35
BsaI GGTCTCn'nnnn_
Cuts at: 0 1000 1209
Size: 1000 209
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 346 1209
Size: 346 863
BseYI C'CCAG_C
Cuts at: 0 161 1102 1209
Size: 161 941 107
Fragments arranged by size:
941 161 107
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 893 1209
Size: 893 316
BsrFI r'CCGG_y
Cuts at: 0 207 1209
Size: 207 1002
BssSI C'ACGA_G
Cuts at: 0 76 1209
Size: 76 1133
BstAPI GCAn_nnn'nTGC
Cuts at: 0 303 1209
Size: 303 906
BstBI TT'CG_AA
Cuts at: 0 745 1209
Size: 745 464
BstYI r'GATC_y
Cuts at: 0 930 1209
Size: 930 279
Bsu36I CC'TnA_GG
Cuts at: 0 329 1209
Size: 329 880
BtsI GCAGTG_nn'
Cuts at: 0 385 1209
Size: 385 824
DraI TTT'AAA
Cuts at: 0 495 1209
Size: 495 714
EaeI y'GGCC_r
Cuts at: 0 123 1209
Size: 123 1086
EarI CTCTTCn'nnn_
Cuts at: 0 531 742 1209
Size: 531 211 467
Fragments arranged by size:
531 467 211
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 180 249 526 649 821 957 1209
Size: 35 145 69 277 123 172 136 252
Fragments arranged by size:
277 252 172 145 136 123 69 35
EcoO109I rG'GnC_Cy
Cuts at: 0 31 1071 1209
Size: 31 1040 138
Fragments arranged by size:
1040 138 31
EcoRI G'AATT_C
Cuts at: 0 1033 1209
Size: 1033 176
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 891 923 1209
Size: 891 32 286
Fragments arranged by size:
891 286 32
HindIII A'AGCT_T
Cuts at: 0 187 1209
Size: 187 1022
MfeI C'AATT_G
Cuts at: 0 232 1209
Size: 232 977
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 889 1209
Size: 36 853 320
Fragments arranged by size:
853 320 36
MscI TGG'CCA
Cuts at: 0 125 1209
Size: 125 1084
MspA1I CmG'CkG
Cuts at: 0 135 504 799 1209
Size: 135 369 295 410
Fragments arranged by size:
410 369 295 135
NheI G'CTAG_C
Cuts at: 0 1195 1209
Size: 1195 14
NsiI A_TGCA'T
Cuts at: 0 917 1209
Size: 917 292
NspI r_CATG'y
Cuts at: 0 841 1209
Size: 841 368
PfoI T'CCnGG_A
Cuts at: 0 850 1209
Size: 850 359
PpuMI rG'GwC_Cy
Cuts at: 0 31 1071 1209
Size: 31 1040 138
Fragments arranged by size:
1040 138 31
PvuII CAG'CTG
Cuts at: 0 504 799 1209
Size: 504 295 410
Fragments arranged by size:
504 410 295
ScaI AGT'ACT
Cuts at: 0 1130 1209
Size: 1130 79
SfcI C'TryA_G
Cuts at: 0 44 1209
Size: 44 1165
SspI AAT'ATT
Cuts at: 0 576 1209
Size: 576 633
StyI C'CwwG_G
Cuts at: 0 942 1209
Size: 942 267
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 20 298 815 1209
Size: 20 278 517 394
Fragments arranged by size:
517 394 278 20
TatI w'GTAC_w
Cuts at: 0 834 1128 1209
Size: 834 294 81
Fragments arranged by size:
834 294 81
Enzymes that do cut:
AccI AcuI AflIII AlfI AlwNI ApoI BaeI BanI
BbsI BfrBI BlpI BmtI BplI BpmI BsaI BseRI
BseYI BspMI BsrFI BssSI BstAPI BstBI BstYI Bsu36I
BtsI DraI EaeI EarI Eco57MI EcoO109I EcoRI Hin4I
HindIII MfeI MmeI MscI MspA1I NheI NsiI NspI
PfoI PpuMI PvuII ScaI SfcI SspI StyI TaqII
TatI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BamHI BanII BbeI BbvCI BcgI BciVI BclI
BglI BglII Bme1580I BmgBI BmrI Bpu10I BpuEI BsaAI
BsaBI BsaHI BsaWI BsaXI BsgI BsiEI BsiHKAI BsiWI
BsmI BsmBI Bsp1286I BspEI BspHI BsrBI BsrDI BsrGI
BssHII BstEII BstXI BstZ17I BtgI BtgZI ClaI CspCI
DraIII DrdI EagI EciI EcoICRI EcoNI EcoRV FalI
FseI FspI FspAI HaeII HincII HpaI KasI KpnI
MluI MslI NaeI NarI NcoI NdeI NgoMIV NotI
NruI PacI PasI PciI PflMI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI StuI SwaI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI