Restriction map for
pF1KB8304
(Linear) (Six-base) MAPSORT of: KIBB8304.seq Check: 3058 from: 1 to: 894
KIBB8304.orf
With 182 enzymes: SgfI *
March 3, 2008 11:04 ..
Acc65I G'GTAC_C
Cuts at: 0 500 894
Size: 500 394
AflII C'TTAA_G
Cuts at: 0 94 163 894
Size: 94 69 731
Fragments arranged by size:
731 94 69
AleI CACnn'nnGTG
Cuts at: 0 547 894
Size: 547 347
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 449 481 894
Size: 449 32 413
Fragments arranged by size:
449 413 32
AlwNI CAG_nnn'CTG
Cuts at: 0 396 894
Size: 396 498
ApaI G_GGCC'C
Cuts at: 0 603 732 894
Size: 603 129 162
Fragments arranged by size:
603 162 129
ApoI r'AATT_y
Cuts at: 0 241 265 894
Size: 241 24 629
Fragments arranged by size:
629 241 24
BanI G'GyrC_C
Cuts at: 0 500 894
Size: 500 394
BanII G_rGCy'C
Cuts at: 0 510 603 732 894
Size: 510 93 129 162
Fragments arranged by size:
510 162 129 93
BglII A'GATC_T
Cuts at: 0 152 255 894
Size: 152 103 639
Fragments arranged by size:
639 152 103
Bme1580I G_kGCm'C
Cuts at: 0 40 134 603 732 894
Size: 40 94 469 129 162
Fragments arranged by size:
469 162 129 94 40
BmrI ACTGGGnnnn_n'
Cuts at: 0 128 894
Size: 128 766
BmtI G_CTAG'C
Cuts at: 0 430 894
Size: 430 464
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 579 632 894
Size: 319 260 53 262
Fragments arranged by size:
319 262 260 53
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 894
Size: 244 650
BsaI GGTCTCn'nnnn_
Cuts at: 0 371 894
Size: 371 523
BsaAI yAC'GTr
Cuts at: 0 41 894
Size: 41 853
BsaWI w'CCGG_w
Cuts at: 0 638 894
Size: 638 256
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 71 101 449 479 894
Size: 71 30 348 30 415
Fragments arranged by size:
415 348 71 30 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 784 894
Size: 232 552 110
Fragments arranged by size:
552 232 110
BsiHKAI G_wGCw'C
Cuts at: 0 510 894
Size: 510 384
BsiWI C'GTAC_G
Cuts at: 0 41 894
Size: 41 853
BsmI GAATG_Cn'
Cuts at: 0 293 894
Size: 293 601
Bsp1286I G_dGCh'C
Cuts at: 0 40 134 510 603 732 894
Size: 40 94 376 93 129 162
Fragments arranged by size:
376 162 129 94 93 40
BspEI T'CCGG_A
Cuts at: 0 638 894
Size: 638 256
BsrGI T'GTAC_A
Cuts at: 0 53 894
Size: 53 841
BstAPI GCAn_nnn'nTGC
Cuts at: 0 798 894
Size: 798 96
BstEII G'GTnAC_C
Cuts at: 0 489 894
Size: 489 405
BstXI CCAn_nnnn'nTGG
Cuts at: 0 549 894
Size: 549 345
BstYI r'GATC_y
Cuts at: 0 152 255 641 894
Size: 152 103 386 253
Fragments arranged by size:
386 253 152 103
EaeI y'GGCC_r
Cuts at: 0 679 827 894
Size: 679 148 67
Fragments arranged by size:
679 148 67
EarI CTCTTCn'nnn_
Cuts at: 0 305 640 894
Size: 305 335 254
Fragments arranged by size:
335 305 254
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 94 894
Size: 94 800
EcoICRI GAG'CTC
Cuts at: 0 508 894
Size: 508 386
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 579 632 894
Size: 319 260 53 262
Fragments arranged by size:
319 262 260 53
EcoO109I rG'GnC_Cy
Cuts at: 0 339 366 600 659 894
Size: 339 27 234 59 235
Fragments arranged by size:
339 235 234 59 27
HaeII r_GCGC'y
Cuts at: 0 94 894
Size: 94 800
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 576 608 894
Size: 576 32 286
Fragments arranged by size:
576 286 32
HincII GTy'rAC
Cuts at: 0 74 894
Size: 74 820
KpnI G_GTAC'C
Cuts at: 0 504 894
Size: 504 390
MscI TGG'CCA
Cuts at: 0 681 894
Size: 681 213
MslI CAynn'nnrTG
Cuts at: 0 547 894
Size: 547 347
MspA1I CmG'CkG
Cuts at: 0 330 547 894
Size: 330 217 347
Fragments arranged by size:
347 330 217
NheI G'CTAG_C
Cuts at: 0 426 894
Size: 426 468
PflMI CCAn_nnn'nTGG
Cuts at: 0 18 486 894
Size: 18 468 408
Fragments arranged by size:
468 408 18
PfoI T'CCnGG_A
Cuts at: 0 857 894
Size: 857 37
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 449 481 894
Size: 449 32 413
Fragments arranged by size:
449 413 32
PpuMI rG'GwC_Cy
Cuts at: 0 366 659 894
Size: 366 293 235
Fragments arranged by size:
366 293 235
PspOMI G'GGCC_C
Cuts at: 0 599 728 894
Size: 599 129 166
Fragments arranged by size:
599 166 129
PvuII CAG'CTG
Cuts at: 0 330 547 894
Size: 330 217 347
Fragments arranged by size:
347 330 217
SacI G_AGCT'C
Cuts at: 0 510 894
Size: 510 384
SanDI GG'GwC_CC
Cuts at: 0 659 894
Size: 659 235
SapI GCTCTTCn'nnn_
Cuts at: 0 640 894
Size: 640 254
ScaI AGT'ACT
Cuts at: 0 138 894
Size: 138 756
SexAI A'CCwGG_T
Cuts at: 0 230 894
Size: 230 664
SmlI C'TyrA_G
Cuts at: 0 94 163 259 894
Size: 94 69 96 635
Fragments arranged by size:
635 96 94 69
SspI AAT'ATT
Cuts at: 0 186 535 894
Size: 186 349 359
Fragments arranged by size:
359 349 186
StyI C'CwwG_G
Cuts at: 0 830 894
Size: 830 64
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 493 894
Size: 493 401
TatI w'GTAC_w
Cuts at: 0 53 136 894
Size: 53 83 758
Fragments arranged by size:
758 83 53
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 71 103 279 311 894
Size: 71 32 176 32 583
Fragments arranged by size:
583 176 71 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 837 894
Size: 837 57
Enzymes that do cut:
Acc65I AflII AleI AloI AlwNI ApaI ApoI BanI
BanII BglII Bme1580I BmrI BmtI BpmI BpuEI BsaI
BsaAI BsaWI BsaXI BsgI BsiHKAI BsiWI BsmI Bsp1286I
BspEI BsrGI BstAPI BstEII BstXI BstYI EaeI EarI
EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I HincII KpnI
MscI MslI MspA1I NheI PflMI PfoI PpiI PpuMI
PspOMI PvuII SacI SanDI SapI ScaI SexAI SmlI
SspI StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflIII AgeI
AhdI AlfI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BlpI BmgBI BplI Bpu10I BsaBI BsaHI
BseRI BseYI BsiEI BsmBI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstBI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI
KasI MfeI MluI MmeI NaeI NarI NcoI NdeI
NgoMIV NotI NruI NsiI NspI PacI PasI PciI
PmeI PmlI PshAI PsiI PspXI PsrI PstI PvuI
RsrII SacII SalI SbfI SfcI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI
Tth111I XbaI XhoI XmaI XmnI ZraI