Restriction map for
pF1KB8302
(Linear) (Six-base) MAPSORT of: KIBB8302.seq Check: 2590 from: 1 to: 675
KIBB8302.orf
With 182 enzymes: SgfI *
March 3, 2008 11:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 8 675
Size: 8 667
AflIII A'CryG_T
Cuts at: 0 509 546 675
Size: 509 37 129
Fragments arranged by size:
509 129 37
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 199 675
Size: 199 476
AlwNI CAG_nnn'CTG
Cuts at: 0 192 675
Size: 192 483
ApoI r'AATT_y
Cuts at: 0 103 166 607 675
Size: 103 63 441 68
Fragments arranged by size:
441 103 68 63
BanI G'GyrC_C
Cuts at: 0 20 675
Size: 20 655
BanII G_rGCy'C
Cuts at: 0 31 675
Size: 31 644
BmrI ACTGGGnnnn_n'
Cuts at: 0 80 258 675
Size: 80 178 417
Fragments arranged by size:
417 178 80
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 270 675
Size: 163 107 405
Fragments arranged by size:
405 163 107
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 260 675
Size: 260 415
BsaAI yAC'GTr
Cuts at: 0 512 549 675
Size: 512 37 126
Fragments arranged by size:
512 126 37
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 253 544 675
Size: 253 291 131
Fragments arranged by size:
291 253 131
BseYI C'CCAG_C
Cuts at: 0 33 220 675
Size: 33 187 455
Fragments arranged by size:
455 187 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 503 675
Size: 503 172
Bsp1286I G_dGCh'C
Cuts at: 0 31 675
Size: 31 644
BspHI T'CATG_A
Cuts at: 0 476 641 675
Size: 476 165 34
Fragments arranged by size:
476 165 34
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 8 177 675
Size: 8 169 498
Fragments arranged by size:
498 169 8
BsrFI r'CCGG_y
Cuts at: 0 128 675
Size: 128 547
BstEII G'GTnAC_C
Cuts at: 0 594 675
Size: 594 81
Bsu36I CC'TnA_GG
Cuts at: 0 134 675
Size: 134 541
DraIII CAC_nnn'GTG
Cuts at: 0 122 675
Size: 122 553
EarI CTCTTCn'nnn_
Cuts at: 0 313 675
Size: 313 362
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 163 270 675
Size: 163 107 405
Fragments arranged by size:
405 163 107
EcoNI CCTnn'n_nnAGG
Cuts at: 0 436 675
Size: 436 239
EcoO109I rG'GnC_Cy
Cuts at: 0 189 534 675
Size: 189 345 141
Fragments arranged by size:
345 189 141
EcoRI G'AATT_C
Cuts at: 0 607 675
Size: 607 68
MspA1I CmG'CkG
Cuts at: 0 33 63 150 224 675
Size: 33 30 87 74 451
Fragments arranged by size:
451 87 74 33 30
PflMI CCAn_nnn'nTGG
Cuts at: 0 570 675
Size: 570 105
PfoI T'CCnGG_A
Cuts at: 0 524 675
Size: 524 151
PmlI CAC'GTG
Cuts at: 0 549 675
Size: 549 126
PpuMI rG'GwC_Cy
Cuts at: 0 189 675
Size: 189 486
PstI C_TGCA'G
Cuts at: 0 155 675
Size: 155 520
PvuII CAG'CTG
Cuts at: 0 63 150 675
Size: 63 87 525
Fragments arranged by size:
525 87 63
SfcI C'TryA_G
Cuts at: 0 151 392 675
Size: 151 241 283
Fragments arranged by size:
283 241 151
SmlI C'TyrA_G
Cuts at: 0 275 675
Size: 275 400
StyI C'CwwG_G
Cuts at: 0 455 538 675
Size: 455 83 137
Fragments arranged by size:
455 137 83
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 330 675
Size: 330 345
TatI w'GTAC_w
Cuts at: 0 506 675
Size: 506 169
Tth111I GACn'n_nGTC
Cuts at: 0 72 667 675
Size: 72 595 8
Fragments arranged by size:
595 72 8
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 67 675
Size: 67 608
Enzymes that do cut:
AarI AflIII AlfI AlwNI ApoI BanI BanII BmrI
BpmI BpuEI BsaAI BseRI BseYI BsgI Bsp1286I BspHI
BspMI BsrFI BstEII Bsu36I DraIII EarI Eco57MI EcoNI
EcoO109I EcoRI MspA1I PflMI PfoI PmlI PpuMI PstI
PvuII SfcI SmlI StyI TaqII TatI Tth111I XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmtI BplI Bpu10I BsaI BsaBI BsaHI BsaWI BsaXI
BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BsrBI BsrDI
BsrGI BssHII BssSI BstAPI BstBI BstXI BstYI BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EciI EcoICRI EcoRV FalI FseI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TstI XbaI
XhoI XmaI XmnI ZraI