Restriction map for
pF1KB8301
(Linear) (Six-base) MAPSORT of: KIBB8301.seq Check: 8535 from: 1 to: 1434
KIBB8301.orf
With 182 enzymes: SgfI *
March 3, 2008 11:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 573 1434
Size: 573 861
AccI GT'mk_AC
Cuts at: 0 371 1346 1353 1434
Size: 371 975 7 81
Fragments arranged by size:
975 371 81 7
AflIII A'CryG_T
Cuts at: 0 548 1434
Size: 548 886
AhdI GACnn_n'nnGTC
Cuts at: 0 1360 1434
Size: 1360 74
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 418 1434
Size: 418 1016
AlwNI CAG_nnn'CTG
Cuts at: 0 10 639 740 1425 1434
Size: 10 629 101 685 9
Fragments arranged by size:
685 629 101 10 9
AvaI C'yCGr_G
Cuts at: 0 464 1068 1434
Size: 464 604 366
Fragments arranged by size:
604 464 366
BanII G_rGCy'C
Cuts at: 0 235 503 1053 1434
Size: 235 268 550 381
Fragments arranged by size:
550 381 268 235
BbsI GAAGACnn'nnnn_
Cuts at: 0 268 668 1434
Size: 268 400 766
Fragments arranged by size:
766 400 268
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1067 1101 1434
Size: 1067 34 333
Fragments arranged by size:
1067 333 34
BglI GCCn_nnn'nGGC
Cuts at: 0 130 1434
Size: 130 1304
BlpI GC'TnA_GC
Cuts at: 0 1015 1434
Size: 1015 419
Bme1580I G_kGCm'C
Cuts at: 0 168 620 1281 1434
Size: 168 452 661 153
Fragments arranged by size:
661 452 168 153
BmrI ACTGGGnnnn_n'
Cuts at: 0 750 987 1268 1434
Size: 750 237 281 166
Fragments arranged by size:
750 281 237 166
BmtI G_CTAG'C
Cuts at: 0 991 1434
Size: 991 443
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 473 649 913 1283 1434
Size: 473 176 264 370 151
Fragments arranged by size:
473 370 264 176 151
Bpu10I CC'TnA_GC
Cuts at: 0 187 271 1434
Size: 187 84 1163
Fragments arranged by size:
1163 187 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 576 1075 1084 1254 1434
Size: 576 499 9 170 180
Fragments arranged by size:
576 499 180 170 9
BsaI GGTCTCn'nnnn_
Cuts at: 0 1202 1434
Size: 1202 232
BsaWI w'CCGG_w
Cuts at: 0 428 503 1200 1434
Size: 428 75 697 234
Fragments arranged by size:
697 428 234 75
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 886 916 1434
Size: 886 30 518
Fragments arranged by size:
886 518 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 294 1198 1434
Size: 294 904 236
Fragments arranged by size:
904 294 236
BseYI C'CCAG_C
Cuts at: 0 90 114 209 1434
Size: 90 24 95 1225
Fragments arranged by size:
1225 95 90 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 919 1434
Size: 919 515
BsiEI CG_ry'CG
Cuts at: 0 153 1218 1434
Size: 153 1065 216
Fragments arranged by size:
1065 216 153
BsiHKAI G_wGCw'C
Cuts at: 0 71 503 1434
Size: 71 432 931
Fragments arranged by size:
931 432 71
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1027 1434
Size: 1027 407
Bsp1286I G_dGCh'C
Cuts at: 0 71 168 235 503 620 1053 1281 1434
Size: 71 97 67 268 117 433 228 153
Fragments arranged by size:
433 268 228 153 117 97 71 67
BspEI T'CCGG_A
Cuts at: 0 503 1200 1434
Size: 503 697 234
Fragments arranged by size:
697 503 234
BspHI T'CATG_A
Cuts at: 0 671 1434
Size: 671 763
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 573 587 1434
Size: 573 14 847
Fragments arranged by size:
847 573 14
BsrBI CCG'CTC
Cuts at: 0 106 1434
Size: 106 1328
BsrFI r'CCGG_y
Cuts at: 0 275 983 1434
Size: 275 708 451
Fragments arranged by size:
708 451 275
BssSI C'ACGA_G
Cuts at: 0 725 1434
Size: 725 709
BstXI CCAn_nnnn'nTGG
Cuts at: 0 228 351 1434
Size: 228 123 1083
Fragments arranged by size:
1083 228 123
BstYI r'GATC_y
Cuts at: 0 1094 1434
Size: 1094 340
BtgI C'CryG_G
Cuts at: 0 1028 1434
Size: 1028 406
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 105 1434
Size: 105 1329
BtsI GCAGTG_nn'
Cuts at: 0 1139 1434
Size: 1139 295
EaeI y'GGCC_r
Cuts at: 0 53 107 353 826 971 1211 1399 1434
Size: 53 54 246 473 145 240 188 35
Fragments arranged by size:
473 246 240 188 145 54 53 35
EarI CTCTTCn'nnn_
Cuts at: 0 928 1434
Size: 928 506
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 850 1434
Size: 850 584
EcoICRI GAG'CTC
Cuts at: 0 501 1434
Size: 501 933
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 473 649 913 1283 1434
Size: 473 176 264 370 151
Fragments arranged by size:
473 370 264 176 151
EcoO109I rG'GnC_Cy
Cuts at: 0 839 1422 1434
Size: 839 583 12
Fragments arranged by size:
839 583 12
EcoRV GAT'ATC
Cuts at: 0 1324 1434
Size: 1324 110
HaeII r_GCGC'y
Cuts at: 0 11 1434
Size: 11 1423
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 135 167 1008 1040 1346 1378 1434
Size: 135 32 841 32 306 32 56
Fragments arranged by size:
841 306 135 56 32 32 32
HincII GTy'rAC
Cuts at: 0 1354 1434
Size: 1354 80
MfeI C'AATT_G
Cuts at: 0 822 1434
Size: 822 612
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 630 1434
Size: 630 804
MscI TGG'CCA
Cuts at: 0 355 828 973 1401 1434
Size: 355 473 145 428 33
Fragments arranged by size:
473 428 355 145 33
MslI CAynn'nnrTG
Cuts at: 0 670 1434
Size: 670 764
MspA1I CmG'CkG
Cuts at: 0 595 749 1030 1367 1434
Size: 595 154 281 337 67
Fragments arranged by size:
595 337 281 154 67
NdeI CA'TA_TG
Cuts at: 0 490 1108 1434
Size: 490 618 326
Fragments arranged by size:
618 490 326
NheI G'CTAG_C
Cuts at: 0 987 1434
Size: 987 447
NspI r_CATG'y
Cuts at: 0 399 537 552 1434
Size: 399 138 15 882
Fragments arranged by size:
882 399 138 15
PciI A'CATG_T
Cuts at: 0 548 1434
Size: 548 886
PflMI CCAn_nnn'nTGG
Cuts at: 0 824 1434
Size: 824 610
PpuMI rG'GwC_Cy
Cuts at: 0 839 1434
Size: 839 595
PstI C_TGCA'G
Cuts at: 0 407 584 1229 1434
Size: 407 177 645 205
Fragments arranged by size:
645 407 205 177
PvuI CG_AT'CG
Cuts at: 0 153 1434
Size: 153 1281
PvuII CAG'CTG
Cuts at: 0 595 1367 1434
Size: 595 772 67
Fragments arranged by size:
772 595 67
SacI G_AGCT'C
Cuts at: 0 503 1434
Size: 503 931
SacII CC_GC'GG
Cuts at: 0 1031 1434
Size: 1031 403
SalI G'TCGA_C
Cuts at: 0 1352 1434
Size: 1352 82
SanDI GG'GwC_CC
Cuts at: 0 839 1434
Size: 839 595
SbfI CC_TGCA'GG
Cuts at: 0 407 584 1229 1434
Size: 407 177 645 205
Fragments arranged by size:
645 407 205 177
ScaI AGT'ACT
Cuts at: 0 718 1434
Size: 718 716
SfcI C'TryA_G
Cuts at: 0 403 580 1225 1434
Size: 403 177 645 209
Fragments arranged by size:
645 403 209 177
SmlI C'TyrA_G
Cuts at: 0 555 1090 1099 1233 1434
Size: 555 535 9 134 201
Fragments arranged by size:
555 535 201 134 9
SpeI A'CTAG_T
Cuts at: 0 1383 1434
Size: 1383 51
StuI AGG'CCT
Cuts at: 0 1231 1300 1434
Size: 1231 69 134
Fragments arranged by size:
1231 134 69
StyI C'CwwG_G
Cuts at: 0 1289 1434
Size: 1289 145
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 88 1434
Size: 88 1346
TatI w'GTAC_w
Cuts at: 0 551 716 1434
Size: 551 165 718
Fragments arranged by size:
718 551 165
Tth111I GACn'n_nGTC
Cuts at: 0 961 1434
Size: 961 473
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 81 1125 1384 1434
Size: 81 1044 259 50
Fragments arranged by size:
1044 259 81 50
Enzymes that do cut:
AarI AccI AflIII AhdI AlfI AlwNI AvaI BanII
BbsI BcgI BglI BlpI Bme1580I BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrFI BssSI BstXI BstYI BtgI BtgZI BtsI EaeI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRV HaeII Hin4I
HincII MfeI MmeI MscI MslI MspA1I NdeI NheI
NspI PciI PflMI PpuMI PstI PvuI PvuII SacI
SacII SalI SanDI SbfI ScaI SfcI SmlI SpeI
StuI StyI TaqII TatI Tth111I XcmI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AgeI AleI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BbvCI BciVI BclI BfrBI
BglII BmgBI BplI BsaAI BsaBI BsaHI BsiWI BsmI
BsrDI BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I
ClaI CspCI DraI DraIII DrdI EagI EcoNI EcoRI
FalI FseI FspI FspAI HindIII HpaI KasI KpnI
MluI NaeI NarI NcoI NgoMIV NotI NruI NsiI
PacI PasI PfoI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI RsrII SapI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SphI SrfI SspI SwaI
TstI XbaI XhoI XmaI XmnI ZraI