(Linear) (Six-base) MAPSORT of: KIBB8297.seq  Check: 4487  from: 1  to: 573



KIBB8297.orf

 With 182 enzymes: SgfI * 

                        January 9, 2009 11:11  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     100     124     573 
   Size:        100      24     449

  Fragments arranged by size: 

                 449     100      24
 
AlwNI CAG_nnn'CTG

Cuts at:      0      78     573 
   Size:         78     495
 
AvaI C'yCGr_G

Cuts at:      0      28     573 
   Size:         28     545
 
BanI G'GyrC_C

Cuts at:      0     232     397     573 
   Size:        232     165     176

  Fragments arranged by size: 

                 232     176     165
 
BanII G_rGCy'C

Cuts at:      0     434     573 
   Size:        434     139
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      13     573 
   Size:         13     560
 
BclI T'GATC_A

Cuts at:      0     404     573 
   Size:        404     169
 
BglI GCCn_nnn'nGGC

Cuts at:      0     119     573 
   Size:        119     454
 
BglII A'GATC_T

Cuts at:      0     458     573 
   Size:        458     115
 
Bme1580I G_kGCm'C

Cuts at:      0     400     573 
   Size:        400     173
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     315     573 
   Size:        315     258
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58     154     376     385     573 
   Size:         58      96     222       9     188

  Fragments arranged by size: 

                 222     188      96      58       9
 
BsaHI Gr'CG_yC

Cuts at:      0     179     573 
   Size:        179     394
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     151     520     573 
   Size:        151     369      53

  Fragments arranged by size: 

                 369     151      53
 
BseYI C'CCAG_C

Cuts at:      0     115     440     573 
   Size:        115     325     133

  Fragments arranged by size: 

                 325     133     115
 
Bsp1286I G_dGCh'C

Cuts at:      0     400     434     573 
   Size:        400      34     139

  Fragments arranged by size: 

                 400     139      34
 
BspHI T'CATG_A

Cuts at:      0     407     573 
   Size:        407     166
 
BstEII G'GTnAC_C

Cuts at:      0     270     573 
   Size:        270     303
 
BstYI r'GATC_y

Cuts at:      0     458     573 
   Size:        458     115
 
BtgI C'CryG_G

Cuts at:      0     279     573 
   Size:        279     294
 
BtsI GCAGTG_nn'

Cuts at:      0      91     562     573 
   Size:         91     471      11

  Fragments arranged by size: 

                 471      91      11
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58     100     124     154     376     385     573 
   Size:         58      42      24      30     222       9     188

  Fragments arranged by size: 

                 222     188      58      42      30      24       9
 
EcoO109I rG'GnC_Cy

Cuts at:      0      42     573 
   Size:         42     531
 
FspI TGC'GCA

Cuts at:      0     415     573 
   Size:        415     158
 
FspAI rTGC'GCAy

Cuts at:      0     415     573 
   Size:        415     158
 
MspA1I CmG'CkG

Cuts at:      0     119     281     573 
   Size:        119     162     292

  Fragments arranged by size: 

                 292     162     119
 
NspI r_CATG'y

Cuts at:      0     567     573 
   Size:        567       6
 
PfoI T'CCnGG_A

Cuts at:      0     245     573 
   Size:        245     328
 
PpuMI rG'GwC_Cy

Cuts at:      0      42     573 
   Size:         42     531
 
PstI C_TGCA'G

Cuts at:      0     557     573 
   Size:        557      16
 
SacII CC_GC'GG

Cuts at:      0     282     573 
   Size:        282     291
 
SfcI C'TryA_G

Cuts at:      0     553     573 
   Size:        553      20
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     258     573 
   Size:        258     315
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      18     573 
   Size:         18     555
 

 Enzymes that do cut:

     AcuI    AlwNI     AvaI     BanI    BanII     BbsI     BclI     BglI
    BglII Bme1580I     BmrI     BpmI    BsaHI    BseRI    BseYI Bsp1286I
    BspHI   BstEII    BstYI     BtgI     BtsI  Eco57MI EcoO109I     FspI
    FspAI   MspA1I     NspI     PfoI    PpuMI     PstI    SacII     SfcI
    TaqII     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI    BbvCI
     BcgI    BciVI    BfrBI     BlpI    BmgBI     BmtI     BplI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI    BstXI  BstZ17I
   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI
     FseI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     StyI     SwaI     TatI     TstI  Tth111I     XbaI     XhoI     XmaI
     XmnI     ZraI