Restriction map for
pF1KB8294
(Linear) (Six-base) MAPSORT of: KIBB8294.seq Check: 3736 from: 1 to: 2073
KIBB8294.orf
With 182 enzymes: SgfI *
March 3, 2008 11:04 ..
Acc65I G'GTAC_C
Cuts at: 0 1912 2073
Size: 1912 161
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 517 919 1184 1300 1817 2073
Size: 517 402 265 116 517 256
Fragments arranged by size:
517 517 402 265 256 116
AfeI AGC'GCT
Cuts at: 0 1984 2073
Size: 1984 89
AleI CACnn'nnGTG
Cuts at: 0 85 190 242 2073
Size: 85 105 52 1831
Fragments arranged by size:
1831 105 85 52
AlwNI CAG_nnn'CTG
Cuts at: 0 137 948 2073
Size: 137 811 1125
Fragments arranged by size:
1125 811 137
ApaI G_GGCC'C
Cuts at: 0 1044 1942 2073
Size: 1044 898 131
Fragments arranged by size:
1044 898 131
ApoI r'AATT_y
Cuts at: 0 888 2073
Size: 888 1185
AvaI C'yCGr_G
Cuts at: 0 550 785 1364 1700 2073
Size: 550 235 579 336 373
Fragments arranged by size:
579 550 373 336 235
AvrII C'CTAG_G
Cuts at: 0 981 2073
Size: 981 1092
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1378 1411 2073
Size: 1378 33 662
Fragments arranged by size:
1378 662 33
BanI G'GyrC_C
Cuts at: 0 24 115 1912 2073
Size: 24 91 1797 161
Fragments arranged by size:
1797 161 91 24
BanII G_rGCy'C
Cuts at: 0 285 340 409 551 561 857 1019 1044
Size: 285 55 69 142 10 296 162 25
Cuts at: 1044 1190 1942 2073
Size: 146 752 131
Fragments arranged by size:
752 296 285 162 146 142 131 69
55 25 10
BbsI GAAGACnn'nnnn_
Cuts at: 0 191 1640 2073
Size: 191 1449 433
Fragments arranged by size:
1449 433 191
BbvCI CC'TCA_GC
Cuts at: 0 529 1769 2073
Size: 529 1240 304
Fragments arranged by size:
1240 529 304
BlpI GC'TnA_GC
Cuts at: 0 284 851 941 1184 1588 2073
Size: 284 567 90 243 404 485
Fragments arranged by size:
567 485 404 284 243 90
Bme1580I G_kGCm'C
Cuts at: 0 27 1044 1942 2073
Size: 27 1017 898 131
Fragments arranged by size:
1017 898 131 27
BmrI ACTGGGnnnn_n'
Cuts at: 0 119 1879 1943 2073
Size: 119 1760 64 130
Fragments arranged by size:
1760 130 119 64
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 304 2073
Size: 304 1769
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 409 529 712 1033 1429 1540 2035 2073
Size: 409 120 183 321 396 111 495 38
Fragments arranged by size:
495 409 396 321 183 120 111 38
Bpu10I CC'TnA_GC
Cuts at: 0 529 655 935 1769 2073
Size: 529 126 280 834 304
Fragments arranged by size:
834 529 304 280 126
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1044 2073
Size: 1044 1029
BsaI GGTCTCn'nnnn_
Cuts at: 0 665 1667 2073
Size: 665 1002 406
Fragments arranged by size:
1002 665 406
BsaBI GATnn'nnATC
Cuts at: 0 145 2073
Size: 145 1928
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 662 692 1666 1696 2073
Size: 662 30 974 30 377
Fragments arranged by size:
974 662 377 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 406 546 937 1105 1339 1486 1585 1674
Size: 406 140 391 168 234 147 99 89
Cuts at: 1674 1739 1858 2073
Size: 65 119 215
Fragments arranged by size:
406 391 234 215 168 147 140 119
99 89 65
BseYI C'CCAG_C
Cuts at: 0 992 1693 2073
Size: 992 701 380
Fragments arranged by size:
992 701 380
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 562 1310 1849 2073
Size: 562 748 539 224
Fragments arranged by size:
748 562 539 224
BsiHKAI G_wGCw'C
Cuts at: 0 285 551 584 857 936 1190 1445 2073
Size: 285 266 33 273 79 254 255 628
Fragments arranged by size:
628 285 273 266 255 254 79 33
Bsp1286I G_dGCh'C
Cuts at: 0 27 285 340 409 551 561 584 857
Size: 27 258 55 69 142 10 23 273
Cuts at: 857 936 1019 1044 1190 1445 1942 2073
Size: 79 83 25 146 255 497 131
Fragments arranged by size:
497 273 258 255 146 142 131 83
79 69 55 27 25 23 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 275 2073
Size: 275 1798
BsrBI CCG'CTC
Cuts at: 0 1233 2073
Size: 1233 840
BsrFI r'CCGG_y
Cuts at: 0 659 1734 2073
Size: 659 1075 339
Fragments arranged by size:
1075 659 339
BstEII G'GTnAC_C
Cuts at: 0 381 2073
Size: 381 1692
BstXI CCAn_nnnn'nTGG
Cuts at: 0 328 2029 2073
Size: 328 1701 44
Fragments arranged by size:
1701 328 44
BstYI r'GATC_y
Cuts at: 0 1274 2073
Size: 1274 799
BtgI C'CryG_G
Cuts at: 0 33 368 624 2073
Size: 33 335 256 1449
Fragments arranged by size:
1449 335 256 33
BtsI GCAGTG_nn'
Cuts at: 0 1881 2073
Size: 1881 192
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1065 1100 2073
Size: 1065 35 973
Fragments arranged by size:
1065 973 35
EaeI y'GGCC_r
Cuts at: 0 1004 1097 1892 2073
Size: 1004 93 795 181
Fragments arranged by size:
1004 795 181 93
EarI CTCTTCn'nnn_
Cuts at: 0 635 904 2073
Size: 635 269 1169
Fragments arranged by size:
1169 635 269
EcoICRI GAG'CTC
Cuts at: 0 283 549 855 1188 2073
Size: 283 266 306 333 885
Fragments arranged by size:
885 333 306 283 266
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 409 517 529 712 919 1033 1184 1300
Size: 409 108 12 183 207 114 151 116
Cuts at: 1300 1429 1540 1817 2035 2073
Size: 129 111 277 218 38
Fragments arranged by size:
409 277 218 207 183 151 129 116
114 111 108 38 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 391 901 985 1522 2073
Size: 391 510 84 537 551
Fragments arranged by size:
551 537 510 391 84
EcoO109I rG'GnC_Cy
Cuts at: 0 777 1040 1331 1938 1939 2055 2073
Size: 777 263 291 607 1 116 18
Fragments arranged by size:
777 607 291 263 116 18 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1096 2001 2073
Size: 1096 905 72
Fragments arranged by size:
1096 905 72
HaeII r_GCGC'y
Cuts at: 0 1986 2073
Size: 1986 87
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 391 423 1411 1443 1609 1641 1666 1698
Size: 391 32 988 32 166 32 25 32
Cuts at: 1698 1765 1792 1797 1824 2073
Size: 67 27 5 27 249
Fragments arranged by size:
988 391 249 166 67 32 32 32
32 27 27 25 5
HincII GTy'rAC
Cuts at: 0 45 1170 2073
Size: 45 1125 903
Fragments arranged by size:
1125 903 45
HpaI GTT'AAC
Cuts at: 0 1170 2073
Size: 1170 903
KpnI G_GTAC'C
Cuts at: 0 1916 2073
Size: 1916 157
MscI TGG'CCA
Cuts at: 0 1099 1894 2073
Size: 1099 795 179
Fragments arranged by size:
1099 795 179
MslI CAynn'nnrTG
Cuts at: 0 85 190 192 242 2027 2073
Size: 85 105 2 50 1785 46
Fragments arranged by size:
1785 105 85 50 46 2
MspA1I CmG'CkG
Cuts at: 0 495 507 999 1579 1768 1825 2073
Size: 495 12 492 580 189 57 248
Fragments arranged by size:
580 495 492 248 189 57 12
NaeI GCC'GGC
Cuts at: 0 661 1736 2073
Size: 661 1075 337
Fragments arranged by size:
1075 661 337
NcoI C'CATG_G
Cuts at: 0 368 624 2073
Size: 368 256 1449
Fragments arranged by size:
1449 368 256
NgoMIV G'CCGG_C
Cuts at: 0 659 1734 2073
Size: 659 1075 339
Fragments arranged by size:
1075 659 339
PflMI CCAn_nnn'nTGG
Cuts at: 0 34 137 1654 2073
Size: 34 103 1517 419
Fragments arranged by size:
1517 419 103 34
PfoI T'CCnGG_A
Cuts at: 0 689 2073
Size: 689 1384
PpuMI rG'GwC_Cy
Cuts at: 0 1331 2055 2073
Size: 1331 724 18
Fragments arranged by size:
1331 724 18
PspOMI G'GGCC_C
Cuts at: 0 1040 1938 2073
Size: 1040 898 135
Fragments arranged by size:
1040 898 135
PstI C_TGCA'G
Cuts at: 0 1250 2073
Size: 1250 823
PvuII CAG'CTG
Cuts at: 0 495 507 1768 1825 2073
Size: 495 12 1261 57 248
Fragments arranged by size:
1261 495 248 57 12
SacI G_AGCT'C
Cuts at: 0 285 551 857 1190 2073
Size: 285 266 306 333 883
Fragments arranged by size:
883 333 306 285 266
SapI GCTCTTCn'nnn_
Cuts at: 0 904 2073
Size: 904 1169
SfcI C'TryA_G
Cuts at: 0 502 1246 2073
Size: 502 744 827
Fragments arranged by size:
827 744 502
SmaI CCC'GGG
Cuts at: 0 1366 2073
Size: 1366 707
SmlI C'TyrA_G
Cuts at: 0 550 1059 1700 2073
Size: 550 509 641 373
Fragments arranged by size:
641 550 509 373
StyI C'CwwG_G
Cuts at: 0 83 361 368 431 624 804 981 2073
Size: 83 278 7 63 193 180 177 1092
Fragments arranged by size:
1092 278 193 180 177 83 63 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2065 2073
Size: 2065 8
TatI w'GTAC_w
Cuts at: 0 214 602 822 1493 2073
Size: 214 388 220 671 580
Fragments arranged by size:
671 580 388 220 214
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 662 694 2073
Size: 662 32 1379
Fragments arranged by size:
1379 662 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 84 375 2073
Size: 84 291 1698
Fragments arranged by size:
1698 291 84
XhoI C'TCGA_G
Cuts at: 0 550 1700 2073
Size: 550 1150 373
Fragments arranged by size:
1150 550 373
XmaI C'CCGG_G
Cuts at: 0 1364 2073
Size: 1364 709
Enzymes that do cut:
Acc65I AcuI AfeI AleI AlwNI ApaI ApoI AvaI
AvrII BaeI BanI BanII BbsI BbvCI BlpI Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaXI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI BsrFI
BstEII BstXI BstYI BtgI BtsI CspCI EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII
HpaI KpnI MscI MslI MspA1I NaeI NcoI NgoMIV
PflMI PfoI PpuMI PspOMI PstI PvuII SacI SapI
SfcI SmaI SmlI StyI TaqII TatI TstI XcmI
XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AflIII AgeI AhdI
AlfI AloI ApaLI AscI AseI AsiSI BamHI BbeI
BcgI BciVI BclI BfrBI BglI BglII BmgBI BmtI
BsaAI BsaHI BsaWI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstZ17I
Bsu36I BtgZI ClaI DraI DraIII DrdI EagI EciI
EcoRI EcoRV FseI FspI FspAI HindIII KasI MfeI
MluI MmeI NarI NdeI NheI NotI NruI NsiI
NspI PacI PasI PciI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI
XmnI ZraI