(Linear) (Six-base) MAPSORT of: KIBB8293.seq  Check: 238  from: 1  to: 906



KIBB8293.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:04  ..

AccI GT'mk_AC

Cuts at:      0     513     906 
   Size:        513     393
 
AclI AA'CG_TT

Cuts at:      0     672     906 
   Size:        672     234
 
AflII C'TTAA_G

Cuts at:      0     709     906 
   Size:        709     197
 
AlwNI CAG_nnn'CTG

Cuts at:      0     754     906 
   Size:        754     152
 
ApoI r'AATT_y

Cuts at:      0     269     819     906 
   Size:        269     550      87

  Fragments arranged by size: 

                 550     269      87
 
BamHI G'GATC_C

Cuts at:      0      82     906 
   Size:         82     824
 
BanII G_rGCy'C

Cuts at:      0     169     906 
   Size:        169     737
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     695     733     906 
   Size:        695      38     173

  Fragments arranged by size: 

                 695     173      38
 
BglII A'GATC_T

Cuts at:      0     255     618     906 
   Size:        255     363     288

  Fragments arranged by size: 

                 363     288     255
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     793     906 
   Size:        793     113
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      90     906 
   Size:         90     816
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     317     421     906 
   Size:        317     104     485

  Fragments arranged by size: 

                 485     317     104
 
BsiHKAI G_wGCw'C

Cuts at:      0     119     906 
   Size:        119     787
 
BsmI GAATG_Cn'

Cuts at:      0     659     879     906 
   Size:        659     220      27

  Fragments arranged by size: 

                 659     220      27
 
Bsp1286I G_dGCh'C

Cuts at:      0     119     169     906 
   Size:        119      50     737

  Fragments arranged by size: 

                 737     119      50
 
BsrDI GCAATG_nn'

Cuts at:      0     236     906 
   Size:        236     670
 
BstBI TT'CG_AA

Cuts at:      0     461     504     906 
   Size:        461      43     402

  Fragments arranged by size: 

                 461     402      43
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     771     906 
   Size:        771     135
 
BstYI r'GATC_y

Cuts at:      0      82     255     618     906 
   Size:         82     173     363     288

  Fragments arranged by size: 

                 363     288     173      82
 
BtsI GCAGTG_nn'

Cuts at:      0      28     906 
   Size:         28     878
 
DraIII CAC_nnn'GTG

Cuts at:      0     340     906 
   Size:        340     566
 
EarI CTCTTCn'nnn_

Cuts at:      0     290     906 
   Size:        290     616
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      92     906 
   Size:         92     814
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     793     906 
   Size:        793     113
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     448     480     595     627     906 
   Size:        448      32     115      32     279

  Fragments arranged by size: 

                 448     279     115      32      32
 
HincII GTy'rAC

Cuts at:      0     210     906 
   Size:        210     696
 
HindIII A'AGCT_T

Cuts at:      0     320     906 
   Size:        320     586
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     443     906 
   Size:        443     463
 
NspI r_CATG'y

Cuts at:      0     104     906 
   Size:        104     802
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     657     689     906 
   Size:        657      32     217

  Fragments arranged by size: 

                 657     217      32
 
PstI C_TGCA'G

Cuts at:      0      62     906 
   Size:         62     844
 
SfcI C'TryA_G

Cuts at:      0      58     530     906 
   Size:         58     472     376

  Fragments arranged by size: 

                 472     376      58
 
SmlI C'TyrA_G

Cuts at:      0     105     709     906 
   Size:        105     604     197

  Fragments arranged by size: 

                 604     197     105
 
SpeI A'CTAG_T

Cuts at:      0     589     906 
   Size:        589     317
 
StuI AGG'CCT

Cuts at:      0     186     906 
   Size:        186     720
 
XbaI T'CTAG_A

Cuts at:      0     621     906 
   Size:        621     285
 
XmnI GAAnn'nnTTC

Cuts at:      0     500     692     906 
   Size:        500     192     214

  Fragments arranged by size: 

                 500     214     192
 

 Enzymes that do cut:

     AccI     AclI    AflII    AlwNI     ApoI    BamHI    BanII     BbsI
    BglII     BpmI    BpuEI     BsgI  BsiHKAI     BsmI Bsp1286I    BsrDI
    BstBI    BstXI    BstYI     BtsI   DraIII     EarI     EciI  Eco57MI
    Hin4I   HincII  HindIII     MmeI     NspI     PpiI     PstI     SfcI
     SmlI     SpeI     StuI     XbaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AcuI     AfeI   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI     BanI     BbeI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI   BstEII  BstZ17I
   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI     DrdI     EaeI
     EagI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII     HpaI     KasI     KpnI     MfeI     MluI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SphI     SrfI     SspI     StyI     SwaI    TaqII
     TatI     TstI  Tth111I     XcmI     XhoI     XmaI     ZraI