Restriction map for
pF1KB8292
(Linear) (Six-base) MAPSORT of: KIBB8292.seq Check: 4164 from: 1 to: 1347
KIBB8292.orf
With 182 enzymes: SgfI *
March 3, 2008 11:04 ..
AflIII A'CryG_T
Cuts at: 0 275 1091 1347
Size: 275 816 256
Fragments arranged by size:
816 275 256
AhdI GACnn_n'nnGTC
Cuts at: 0 564 693 1347
Size: 564 129 654
Fragments arranged by size:
654 564 129
AleI CACnn'nnGTG
Cuts at: 0 152 1094 1347
Size: 152 942 253
Fragments arranged by size:
942 253 152
AlwNI CAG_nnn'CTG
Cuts at: 0 1326 1347
Size: 1326 21
ApaLI G'TGCA_C
Cuts at: 0 927 1347
Size: 927 420
ApoI r'AATT_y
Cuts at: 0 100 136 1347
Size: 100 36 1211
Fragments arranged by size:
1211 100 36
AvaI C'yCGr_G
Cuts at: 0 546 1347
Size: 546 801
BbsI GAAGACnn'nnnn_
Cuts at: 0 856 1149 1347
Size: 856 293 198
Fragments arranged by size:
856 293 198
BciVI GTATCCnnnnn_n'
Cuts at: 0 358 1347
Size: 358 989
BclI T'GATC_A
Cuts at: 0 48 1347
Size: 48 1299
BglI GCCn_nnn'nGGC
Cuts at: 0 838 1347
Size: 838 509
Bme1580I G_kGCm'C
Cuts at: 0 931 1035 1347
Size: 931 104 312
Fragments arranged by size:
931 312 104
BmrI ACTGGGnnnn_n'
Cuts at: 0 1253 1347
Size: 1253 94
Bpu10I CC'TnA_GC
Cuts at: 0 583 1347
Size: 583 764
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 223 692 1111 1347
Size: 223 469 419 236
Fragments arranged by size:
469 419 236 223
BsaI GGTCTCn'nnnn_
Cuts at: 0 664 1347
Size: 664 683
BseYI C'CCAG_C
Cuts at: 0 612 1347
Size: 612 735
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1164 1347
Size: 1164 183
BsiHKAI G_wGCw'C
Cuts at: 0 147 931 1347
Size: 147 784 416
Fragments arranged by size:
784 416 147
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1324 1347
Size: 1324 23
Bsp1286I G_dGCh'C
Cuts at: 0 147 931 1035 1347
Size: 147 784 104 312
Fragments arranged by size:
784 312 147 104
BspHI T'CATG_A
Cuts at: 0 1134 1347
Size: 1134 213
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1239 1347
Size: 1239 108
BsrGI T'GTAC_A
Cuts at: 0 278 1347
Size: 278 1069
BssSI C'ACGA_G
Cuts at: 0 1219 1347
Size: 1219 128
BstEII G'GTnAC_C
Cuts at: 0 811 1251 1347
Size: 811 440 96
Fragments arranged by size:
811 440 96
BstXI CCAn_nnnn'nTGG
Cuts at: 0 844 1347
Size: 844 503
BtgI C'CryG_G
Cuts at: 0 665 1347
Size: 665 682
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1315 1347
Size: 1315 32
DraI TTT'AAA
Cuts at: 0 183 1347
Size: 183 1164
EaeI y'GGCC_r
Cuts at: 0 648 1347
Size: 648 699
EarI CTCTTCn'nnn_
Cuts at: 0 254 1347
Size: 254 1093
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 916 1347
Size: 916 431
EcoO109I rG'GnC_Cy
Cuts at: 0 264 695 1347
Size: 264 431 652
Fragments arranged by size:
652 431 264
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 550 582 679 711 1347
Size: 550 32 97 32 636
Fragments arranged by size:
636 550 97 32 32
HincII GTy'rAC
Cuts at: 0 315 1347
Size: 315 1032
HindIII A'AGCT_T
Cuts at: 0 187 199 1347
Size: 187 12 1148
Fragments arranged by size:
1148 187 12
HpaI GTT'AAC
Cuts at: 0 315 1347
Size: 315 1032
MfeI C'AATT_G
Cuts at: 0 639 1347
Size: 639 708
MslI CAynn'nnrTG
Cuts at: 0 16 150 152 1067 1094 1347
Size: 16 134 2 915 27 253
Fragments arranged by size:
915 253 134 27 16 2
MspA1I CmG'CkG
Cuts at: 0 1326 1347
Size: 1326 21
NcoI C'CATG_G
Cuts at: 0 665 1347
Size: 665 682
NspI r_CATG'y
Cuts at: 0 279 1095 1347
Size: 279 816 252
Fragments arranged by size:
816 279 252
PciI A'CATG_T
Cuts at: 0 275 1091 1347
Size: 275 816 256
Fragments arranged by size:
816 275 256
PpuMI rG'GwC_Cy
Cuts at: 0 264 695 1347
Size: 264 431 652
Fragments arranged by size:
652 431 264
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 262 294 1347
Size: 262 32 1053
Fragments arranged by size:
1053 262 32
PstI C_TGCA'G
Cuts at: 0 172 1347
Size: 172 1175
PvuII CAG'CTG
Cuts at: 0 1326 1347
Size: 1326 21
SanDI GG'GwC_CC
Cuts at: 0 695 1347
Size: 695 652
SfcI C'TryA_G
Cuts at: 0 168 996 1341 1347
Size: 168 828 345 6
Fragments arranged by size:
828 345 168 6
SmlI C'TyrA_G
Cuts at: 0 202 707 1126 1347
Size: 202 505 419 221
Fragments arranged by size:
505 419 221 202
StyI C'CwwG_G
Cuts at: 0 665 1184 1196 1347
Size: 665 519 12 151
Fragments arranged by size:
665 519 151 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 210 487 1347
Size: 210 277 860
Fragments arranged by size:
860 277 210
TatI w'GTAC_w
Cuts at: 0 278 1347
Size: 278 1069
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 352 384 1347
Size: 352 32 963
Fragments arranged by size:
963 352 32
Enzymes that do cut:
AflIII AhdI AleI AlwNI ApaLI ApoI AvaI BbsI
BciVI BclI BglI Bme1580I BmrI Bpu10I BpuEI BsaI
BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrGI
BssSI BstEII BstXI BtgI BtgZI DraI EaeI EarI
EciI EcoO109I Hin4I HincII HindIII HpaI MfeI MslI
MspA1I NcoI NspI PciI PpuMI PsrI PstI PvuII
SanDI SfcI SmlI StyI TaqII TatI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AlfI AloI ApaI AscI AseI AsiSI AvrII
BaeI BamHI BanI BanII BbeI BbvCI BcgI BfrBI
BglII BlpI BmgBI BmtI BplI BpmI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BsiEI BsiWI BsmI BspEI
BsrBI BsrDI BsrFI BssHII BstAPI BstBI BstYI BstZ17I
Bsu36I BtsI ClaI CspCI DraIII DrdI EagI EcoICRI
Eco57MI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HaeII KasI KpnI MluI MmeI MscI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PvuI RsrII SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI