Restriction map for
pF1KB8288
(Linear) (Six-base) MAPSORT of: KIBB8288.seq Check: 7652 from: 1 to: 1221
KIBB8288.orf
With 182 enzymes: SgfI *
March 3, 2008 11:04 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 1221
Size: 292 929
AhdI GACnn_n'nnGTC
Cuts at: 0 1028 1221
Size: 1028 193
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 202 1221
Size: 202 1019
AlwNI CAG_nnn'CTG
Cuts at: 0 30 42 332 619 1221
Size: 30 12 290 287 602
Fragments arranged by size:
602 290 287 30 12
ApoI r'AATT_y
Cuts at: 0 94 1221
Size: 94 1127
AseI AT'TA_AT
Cuts at: 0 443 1221
Size: 443 778
AvaI C'yCGr_G
Cuts at: 0 966 1221
Size: 966 255
BamHI G'GATC_C
Cuts at: 0 279 970 1221
Size: 279 691 251
Fragments arranged by size:
691 279 251
BanI G'GyrC_C
Cuts at: 0 922 1221
Size: 922 299
BanII G_rGCy'C
Cuts at: 0 186 818 838 1221
Size: 186 632 20 383
Fragments arranged by size:
632 383 186 20
BbsI GAAGACnn'nnnn_
Cuts at: 0 518 1221
Size: 518 703
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 912 946 1017 1051 1221
Size: 912 34 71 34 170
Fragments arranged by size:
912 170 71 34 34
BclI T'GATC_A
Cuts at: 0 545 1221
Size: 545 676
BglI GCCn_nnn'nGGC
Cuts at: 0 475 640 1221
Size: 475 165 581
Fragments arranged by size:
581 475 165
BglII A'GATC_T
Cuts at: 0 662 1221
Size: 662 559
BlpI GC'TnA_GC
Cuts at: 0 175 1221
Size: 175 1046
BmgBI CAC'GTC
Cuts at: 0 579 611 1221
Size: 579 32 610
Fragments arranged by size:
610 579 32
BmrI ACTGGGnnnn_n'
Cuts at: 0 1160 1221
Size: 1160 61
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 517 802 832 1026 1077 1172 1221
Size: 517 285 30 194 51 95 49
Fragments arranged by size:
517 285 194 95 51 49 30
BsaHI Gr'CG_yC
Cuts at: 0 340 467 628 1221
Size: 340 127 161 593
Fragments arranged by size:
593 340 161 127
BsaWI w'CCGG_w
Cuts at: 0 345 1221
Size: 345 876
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 834 864 919 949 1221
Size: 834 30 55 30 272
Fragments arranged by size:
834 272 55 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 94 514 657 1221
Size: 94 420 143 564
Fragments arranged by size:
564 420 143 94
BseYI C'CCAG_C
Cuts at: 0 954 981 1221
Size: 954 27 240
Fragments arranged by size:
954 240 27
BsiHKAI G_wGCw'C
Cuts at: 0 917 1221
Size: 917 304
BsmBI CGTCTCn'nnnn_
Cuts at: 0 347 657 1221
Size: 347 310 564
Fragments arranged by size:
564 347 310
Bsp1286I G_dGCh'C
Cuts at: 0 186 818 838 917 1221
Size: 186 632 20 79 304
Fragments arranged by size:
632 304 186 79 20
BspEI T'CCGG_A
Cuts at: 0 345 1221
Size: 345 876
BsrFI r'CCGG_y
Cuts at: 0 588 1221
Size: 588 633
BsrGI T'GTAC_A
Cuts at: 0 242 1221
Size: 242 979
BssSI C'ACGA_G
Cuts at: 0 393 1221
Size: 393 828
BstAPI GCAn_nnn'nTGC
Cuts at: 0 42 1221
Size: 42 1179
BstXI CCAn_nnnn'nTGG
Cuts at: 0 591 888 1221
Size: 591 297 333
Fragments arranged by size:
591 333 297
BstYI r'GATC_y
Cuts at: 0 266 279 662 970 1221
Size: 266 13 383 308 251
Fragments arranged by size:
383 308 266 251 13
BtgI C'CryG_G
Cuts at: 0 524 1221
Size: 524 697
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 924 1221
Size: 924 297
BtsI GCAGTG_nn'
Cuts at: 0 1033 1132 1221
Size: 1033 99 89
Fragments arranged by size:
1033 99 89
DraIII CAC_nnn'GTG
Cuts at: 0 20 291 1221
Size: 20 271 930
Fragments arranged by size:
930 271 20
DrdI GACnn_nn'nnGTC
Cuts at: 0 577 1221
Size: 577 644
EaeI y'GGCC_r
Cuts at: 0 122 377 656 1143 1221
Size: 122 255 279 487 78
Fragments arranged by size:
487 279 255 122 78
EarI CTCTTCn'nnn_
Cuts at: 0 781 863 1221
Size: 781 82 358
Fragments arranged by size:
781 358 82
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 621 693 1221
Size: 621 72 528
Fragments arranged by size:
621 528 72
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 517 802 832 1026 1077 1172 1221
Size: 292 225 285 30 194 51 95 49
Fragments arranged by size:
292 285 225 194 95 51 49 30
EcoRI G'AATT_C
Cuts at: 0 94 1221
Size: 94 1127
HaeII r_GCGC'y
Cuts at: 0 904 1221
Size: 904 317
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 267 299 834 866 919 951 1014 1046
Size: 267 32 535 32 53 32 63 32
Cuts at: 1046 1221
Size: 175
Fragments arranged by size:
535 267 175 63 53 32 32 32
32
MscI TGG'CCA
Cuts at: 0 124 1145 1221
Size: 124 1021 76
Fragments arranged by size:
1021 124 76
MslI CAynn'nnrTG
Cuts at: 0 1153 1221
Size: 1153 68
MspA1I CmG'CkG
Cuts at: 0 909 1083 1221
Size: 909 174 138
Fragments arranged by size:
909 174 138
NspI r_CATG'y
Cuts at: 0 1152 1221
Size: 1152 69
PflMI CCAn_nnn'nTGG
Cuts at: 0 131 1221
Size: 131 1090
PfoI T'CCnGG_A
Cuts at: 0 260 779 1221
Size: 260 519 442
Fragments arranged by size:
519 442 260
PstI C_TGCA'G
Cuts at: 0 323 455 1221
Size: 323 132 766
Fragments arranged by size:
766 323 132
ScaI AGT'ACT
Cuts at: 0 1193 1221
Size: 1193 28
SfcI C'TryA_G
Cuts at: 0 319 451 1221
Size: 319 132 770
Fragments arranged by size:
770 319 132
StuI AGG'CCT
Cuts at: 0 422 1221
Size: 422 799
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 713 1221
Size: 713 508
TatI w'GTAC_w
Cuts at: 0 242 425 1191 1221
Size: 242 183 766 30
Fragments arranged by size:
766 242 183 30
Tth111I GACn'n_nGTC
Cuts at: 0 846 1221
Size: 846 375
Enzymes that do cut:
AcuI AhdI AlfI AlwNI ApoI AseI AvaI BamHI
BanI BanII BbsI BcgI BclI BglI BglII BlpI
BmgBI BmrI BpmI BsaHI BsaWI BsaXI BseRI BseYI
BsiHKAI BsmBI Bsp1286I BspEI BsrFI BsrGI BssSI BstAPI
BstXI BstYI BtgI BtgZI BtsI DraIII DrdI EaeI
EarI EciI Eco57MI EcoRI HaeII Hin4I MscI MslI
MspA1I NspI PflMI PfoI PstI ScaI SfcI StuI
TaqII TatI Tth111I
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AloI ApaI ApaLI AscI AsiSI AvrII
BaeI BbeI BbvCI BciVI BfrBI Bme1580I BmtI BplI
Bpu10I BpuEI BsaI BsaAI BsaBI BsgI BsiEI BsiWI
BsmI BspHI BspMI BsrBI BsrDI BssHII BstBI BstEII
BstZ17I Bsu36I ClaI CspCI DraI EagI EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StyI SwaI TstI XbaI XcmI XhoI XmaI XmnI
ZraI