(Linear) (Six-base) MAPSORT of: KIBB8286.seq  Check: 9909  from: 1  to: 1197



KIBB8286.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:04  ..

AclI AA'CG_TT

Cuts at:      0     483    1197 
   Size:        483     714
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     340     938    1197 
   Size:        340     598     259

  Fragments arranged by size: 

                 598     340     259
 
AflII C'TTAA_G

Cuts at:      0     942    1197 
   Size:        942     255
 
AflIII A'CryG_T

Cuts at:      0     397    1197 
   Size:        397     800
 
AlwNI CAG_nnn'CTG

Cuts at:      0     318     835    1197 
   Size:        318     517     362

  Fragments arranged by size: 

                 517     362     318
 
ApoI r'AATT_y

Cuts at:      0     646     769    1197 
   Size:        646     123     428

  Fragments arranged by size: 

                 646     428     123
 
AvaI C'yCGr_G

Cuts at:      0     406    1046    1197 
   Size:        406     640     151

  Fragments arranged by size: 

                 640     406     151
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     919     952    1197 
   Size:        919      33     245

  Fragments arranged by size: 

                 919     245      33
 
BanI G'GyrC_C

Cuts at:      0      63    1197 
   Size:         63    1134
 
BmtI G_CTAG'C

Cuts at:      0     350     368    1197 
   Size:        350      18     829

  Fragments arranged by size: 

                 829     350      18
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     302    1197 
   Size:        302     895
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1137    1197 
   Size:       1137      60
 
BsaAI yAC'GTr

Cuts at:      0     400    1197 
   Size:        400     797
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     387     417    1197 
   Size:        387      30     780

  Fragments arranged by size: 

                 780     387      30
 
BsiHKAI G_wGCw'C

Cuts at:      0     745    1197 
   Size:        745     452
 
Bsp1286I G_dGCh'C

Cuts at:      0     745    1197 
   Size:        745     452
 
BsrFI r'CCGG_y

Cuts at:      0     661    1197 
   Size:        661     536
 
BssSI C'ACGA_G

Cuts at:      0     386    1197 
   Size:        386     811
 
BstYI r'GATC_y

Cuts at:      0     429    1197 
   Size:        429     768
 
Bsu36I CC'TnA_GG

Cuts at:      0      15    1197 
   Size:         15    1182
 
DraI TTT'AAA

Cuts at:      0     717    1076    1127    1197 
   Size:        717     359      51      70

  Fragments arranged by size: 

                 717     359      70      51
 
EarI CTCTTCn'nnn_

Cuts at:      0     917    1142    1197 
   Size:        917     225      55

  Fragments arranged by size: 

                 917     225      55
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     302     340     938    1197 
   Size:        302      38     598     259

  Fragments arranged by size: 

                 598     302     259      38
 
EcoO109I rG'GnC_Cy

Cuts at:      0     219     315    1197 
   Size:        219      96     882

  Fragments arranged by size: 

                 882     219      96
 
EcoRI G'AATT_C

Cuts at:      0     769    1197 
   Size:        769     428
 
EcoRV GAT'ATC

Cuts at:      0     382    1197 
   Size:        382     815
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     957    1197 
   Size:        957     240
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     810     824     842     856    1197 
   Size:        810      14      18      14     341

  Fragments arranged by size: 

                 810     341      18      14      14
 
HincII GTy'rAC

Cuts at:      0     590     782    1197 
   Size:        590     192     415

  Fragments arranged by size: 

                 590     415     192
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      97     537    1197 
   Size:         97     440     660

  Fragments arranged by size: 

                 660     440      97
 
NdeI CA'TA_TG

Cuts at:      0      83    1197 
   Size:         83    1114
 
NheI G'CTAG_C

Cuts at:      0     346     364    1197 
   Size:        346      18     833

  Fragments arranged by size: 

                 833     346      18
 
PfoI T'CCnGG_A

Cuts at:      0     828    1197 
   Size:        828     369
 
PpuMI rG'GwC_Cy

Cuts at:      0     219     315    1197 
   Size:        219      96     882

  Fragments arranged by size: 

                 882     219      96
 
ScaI AGT'ACT

Cuts at:      0    1071    1122    1197 
   Size:       1071      51      75

  Fragments arranged by size: 

                1071      75      51
 
SmlI C'TyrA_G

Cuts at:      0     942    1116    1197 
   Size:        942     174      81

  Fragments arranged by size: 

                 942     174      81
 
SspI AAT'ATT

Cuts at:      0     474    1197 
   Size:        474     723
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     494    1197 
   Size:        494     703
 
TatI w'GTAC_w

Cuts at:      0     913    1069    1120    1197 
   Size:        913     156      51      77

  Fragments arranged by size: 

                 913     156      77      51
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      74    1197 
   Size:         74    1123
 
XmnI GAAnn'nnTTC

Cuts at:      0     325    1197 
   Size:        325     872
 

 Enzymes that do cut:

     AclI     AcuI    AflII   AflIII    AlwNI     ApoI     AvaI     BaeI
     BanI     BmtI     BpmI    BpuEI    BsaAI    BsaXI  BsiHKAI Bsp1286I
    BsrFI    BssSI    BstYI   Bsu36I     DraI     EarI  Eco57MI EcoO109I
    EcoRI    EcoRV     FalI    Hin4I   HincII     MmeI     NdeI     NheI
     PfoI    PpuMI     ScaI     SmlI     SspI    TaqII     TatI     XcmI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AfeI     AgeI     AhdI     AleI
     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI    AvrII
    BamHI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI
   Bpu10I     BsaI    BsaBI    BsaHI    BsaWI    BseRI    BseYI     BsgI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrGI   BssHII   BstAPI    BstBI   BstEII    BstXI  BstZ17I
     BtgI    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI     EaeI
     EagI     EciI  EcoICRI    EcoNI     FseI     FspI    FspAI    HaeII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     StuI     StyI     SwaI     TstI  Tth111I     XbaI
     XhoI     XmaI     ZraI