(Linear) (Six-base) MAPSORT of: KIBB8280.seq  Check: 7906  from: 1  to: 570



KIBB8280.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:04  ..

AatII G_ACGT'C

Cuts at:      0     441     570 
   Size:        441     129
 
Acc65I G'GTAC_C

Cuts at:      0     551     570 
   Size:        551      19
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     520     570 
   Size:        520      50
 
AlwNI CAG_nnn'CTG

Cuts at:      0     385     570 
   Size:        385     185
 
ApoI r'AATT_y

Cuts at:      0      33     570 
   Size:         33     537
 
AvaI C'yCGr_G

Cuts at:      0      59      79     570 
   Size:         59      20     491

  Fragments arranged by size: 

                 491      59      20
 
BanI G'GyrC_C

Cuts at:      0     551     570 
   Size:        551      19
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     228     462     570 
   Size:        228     234     108

  Fragments arranged by size: 

                 234     228     108
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     406     570 
   Size:        406     164
 
BglII A'GATC_T

Cuts at:      0      83     570 
   Size:         83     487
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     146     570 
   Size:        146     424
 
BsaHI Gr'CG_yC

Cuts at:      0     438     570 
   Size:        438     132
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     318     348     570 
   Size:        318      30     222

  Fragments arranged by size: 

                 318     222      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     307     570 
   Size:        307     263
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     448     570 
   Size:        448     122
 
BsmI GAATG_Cn'

Cuts at:      0     240     463     510     570 
   Size:        240     223      47      60

  Fragments arranged by size: 

                 240     223      60      47
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     305     445     570 
   Size:        305     140     125

  Fragments arranged by size: 

                 305     140     125
 
BssSI C'ACGA_G

Cuts at:      0     112     570 
   Size:        112     458
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     405     570 
   Size:        405     165
 
BstYI r'GATC_y

Cuts at:      0      83     570 
   Size:         83     487
 
Bsu36I CC'TnA_GG

Cuts at:      0     370     378     570 
   Size:        370       8     192

  Fragments arranged by size: 

                 370     192       8
 
BtsI GCAGTG_nn'

Cuts at:      0     288     570 
   Size:        288     282
 
EaeI y'GGCC_r

Cuts at:      0     462     570 
   Size:        462     108
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     146     520     570 
   Size:        146     374      50

  Fragments arranged by size: 

                 374     146      50
 
EcoO109I rG'GnC_Cy

Cuts at:      0     374     570 
   Size:        374     196
 
HaeII r_GCGC'y

Cuts at:      0     362     570 
   Size:        362     208
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     283     315     400     428     432     460     570 
   Size:        283      32      85      28       4      28     110

  Fragments arranged by size: 

                 283     110      85      32      28      28       4
 
HincII GTy'rAC

Cuts at:      0     210     570 
   Size:        210     360
 
KpnI G_GTAC'C

Cuts at:      0     555     570 
   Size:        555      15
 
MslI CAynn'nnrTG

Cuts at:      0     403     570 
   Size:        403     167
 
MspA1I CmG'CkG

Cuts at:      0     269     570 
   Size:        269     301
 
PvuII CAG'CTG

Cuts at:      0     269     570 
   Size:        269     301
 
SfcI C'TryA_G

Cuts at:      0     362     474     570 
   Size:        362     112      96

  Fragments arranged by size: 

                 362     112      96
 
StuI AGG'CCT

Cuts at:      0     368     570 
   Size:        368     202
 
StyI C'CwwG_G

Cuts at:      0      89     226     570 
   Size:         89     137     344

  Fragments arranged by size: 

                 344     137      89
 
TatI w'GTAC_w

Cuts at:      0       4     570 
   Size:          4     566
 
XbaI T'CTAG_A

Cuts at:      0      37     570 
   Size:         37     533
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     111     570 
   Size:        111     459
 
ZraI GAC'GTC

Cuts at:      0     439     570 
   Size:        439     131
 

 Enzymes that do cut:

    AatII   Acc65I     AcuI    AlwNI     ApoI     AvaI     BanI     BbsI
    BciVI    BglII     BpmI    BsaHI    BsaXI    BseRI     BsgI     BsmI
    BsmBI    BssSI    BstXI    BstYI   Bsu36I     BtsI     EaeI  Eco57MI
 EcoO109I    HaeII    Hin4I   HincII     KpnI     MslI   MspA1I    PvuII
     SfcI     StuI     StyI     TatI     XbaI     XcmI     ZraI

 Enzymes that do not cut: 

     AarI     AccI     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI    BanII     BbeI    BbvCI     BcgI     BclI
    BfrBI     BglI     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BseYI    BsiEI
  BsiHKAI    BsiWI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII   BstAPI    BstBI   BstEII  BstZ17I     BtgI
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI  HindIII     HpaI     KasI     MfeI     MluI     MmeI     MscI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI    TaqII
     TstI  Tth111I     XhoI     XmaI     XmnI