(Linear) (Six-base) MAPSORT of: KIBB8278.seq  Check: 3654  from: 1  to: 978



KIBB8278.orf

 With 182 enzymes: SgfI * 

                        June 26, 2008 10:50  ..

AccI GT'mk_AC

Cuts at:      0     102     236     978 
   Size:        102     134     742

  Fragments arranged by size: 

                 742     134     102
 
AflII C'TTAA_G

Cuts at:      0     522     978 
   Size:        522     456
 
AflIII A'CryG_T

Cuts at:      0     121     978 
   Size:        121     857
 
ApoI r'AATT_y

Cuts at:      0     406     978 
   Size:        406     572
 
BfrBI ATG'CAT

Cuts at:      0       3     978 
   Size:          3     975
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     818     978 
   Size:        818     160
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     160     663     758     815     978 
   Size:        160     503      95      57     163

  Fragments arranged by size: 

                 503     163     160      95      57
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     136     978 
   Size:        136     842
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     356     481     601     833     978 
   Size:        356     125     120     232     145

  Fragments arranged by size: 

                 356     232     145     125     120
 
BseYI C'CCAG_C

Cuts at:      0     498     780     898     978 
   Size:        498     282     118      80

  Fragments arranged by size: 

                 498     282     118      80
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     856     978 
   Size:        856     122
 
BsrDI GCAATG_nn'

Cuts at:      0     512     978 
   Size:        512     466
 
BstEII G'GTnAC_C

Cuts at:      0     904     978 
   Size:        904      74
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     721     978 
   Size:        721     257
 
BstZ17I GTA'TAC

Cuts at:      0     237     978 
   Size:        237     741
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     647     682     978 
   Size:        647      35     296

  Fragments arranged by size: 

                 647     296      35
 
DraI TTT'AAA

Cuts at:      0     599     978 
   Size:        599     379
 
EarI CTCTTCn'nnn_

Cuts at:      0     268     810     978 
   Size:        268     542     168

  Fragments arranged by size: 

                 542     268     168
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     160     663     758     815     978 
   Size:        160     503      95      57     163

  Fragments arranged by size: 

                 503     163     160      95      57
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     645     978 
   Size:        645     333
 
EcoRI G'AATT_C

Cuts at:      0     406     978 
   Size:        406     572
 
HindIII A'AGCT_T

Cuts at:      0     393     978 
   Size:        393     585
 
MspA1I CmG'CkG

Cuts at:      0     902     978 
   Size:        902      76
 
NsiI A_TGCA'T

Cuts at:      0       5     978 
   Size:          5     973
 
PsiI TTA'TAA

Cuts at:      0     373     978 
   Size:        373     605
 
ScaI AGT'ACT

Cuts at:      0     828     873     978 
   Size:        828      45     105

  Fragments arranged by size: 

                 828     105      45
 
SmlI C'TyrA_G

Cuts at:      0     522     978 
   Size:        522     456
 
TatI w'GTAC_w

Cuts at:      0     826     871     978 
   Size:        826      45     107

  Fragments arranged by size: 

                 826     107      45
 

 Enzymes that do cut:

     AccI    AflII   AflIII     ApoI    BfrBI     BmrI     BpmI     BsaI
    BseRI    BseYI     BsgI    BsrDI   BstEII    BstXI  BstZ17I    CspCI
     DraI     EarI  Eco57MI    EcoNI    EcoRI  HindIII   MspA1I     NsiI
     PsiI     ScaI     SmlI     TatI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmtI     BplI   Bpu10I    BpuEI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI    BstYI   Bsu36I     BtgI    BtgZI     BtsI
     ClaI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI EcoO109I
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI