Restriction map for
pF1KB8273
(Linear) (Six-base) MAPSORT of: KIBB8273.seq Check: 3762 from: 1 to: 831
KIBB8273.orf
With 182 enzymes: SgfI *
March 3, 2008 11:04 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 212 397 831
Size: 212 185 434
Fragments arranged by size:
434 212 185
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 723 756 831
Size: 723 33 75
Fragments arranged by size:
723 75 33
BanII G_rGCy'C
Cuts at: 0 323 634 831
Size: 323 311 197
Fragments arranged by size:
323 311 197
BbsI GAAGACnn'nnnn_
Cuts at: 0 337 831
Size: 337 494
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 117 151 831
Size: 117 34 680
Fragments arranged by size:
680 117 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 91 831
Size: 91 740
Bme1580I G_kGCm'C
Cuts at: 0 704 831
Size: 704 127
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 342 831
Size: 342 489
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 805 831
Size: 337 468 26
Fragments arranged by size:
468 337 26
BsaWI w'CCGG_w
Cuts at: 0 116 634 831
Size: 116 518 197
Fragments arranged by size:
518 197 116
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 316 346 425 455 616 646 831
Size: 316 30 79 30 161 30 185
Fragments arranged by size:
316 185 161 79 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 583 586 770 831
Size: 583 3 184 61
Fragments arranged by size:
583 184 61 3
BseYI C'CCAG_C
Cuts at: 0 139 265 296 831
Size: 139 126 31 535
Fragments arranged by size:
535 139 126 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 434 831
Size: 434 397
BsiHKAI G_wGCw'C
Cuts at: 0 323 831
Size: 323 508
BsmI GAATG_Cn'
Cuts at: 0 69 831
Size: 69 762
Bsp1286I G_dGCh'C
Cuts at: 0 323 634 704 831
Size: 323 311 70 127
Fragments arranged by size:
323 311 127 70
BspEI T'CCGG_A
Cuts at: 0 116 634 831
Size: 116 518 197
Fragments arranged by size:
518 197 116
BsrDI GCAATG_nn'
Cuts at: 0 536 831
Size: 536 295
BsrFI r'CCGG_y
Cuts at: 0 728 831
Size: 728 103
BstXI CCAn_nnnn'nTGG
Cuts at: 0 588 831
Size: 588 243
BtgI C'CryG_G
Cuts at: 0 506 831
Size: 506 325
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 120 588 831
Size: 120 468 243
Fragments arranged by size:
468 243 120
DrdI GACnn_nn'nnGTC
Cuts at: 0 655 831
Size: 655 176
EaeI y'GGCC_r
Cuts at: 0 339 726 831
Size: 339 387 105
Fragments arranged by size:
387 339 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 676 831
Size: 676 155
EcoICRI GAG'CTC
Cuts at: 0 321 831
Size: 321 510
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 212 337 397 805 831
Size: 212 125 60 408 26
Fragments arranged by size:
408 212 125 60 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 351 383 440 472 616 648 831
Size: 351 32 57 32 144 32 183
Fragments arranged by size:
351 183 144 57 32 32 32
HindIII A'AGCT_T
Cuts at: 0 103 831
Size: 103 728
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 12 335 831
Size: 12 323 496
Fragments arranged by size:
496 323 12
MslI CAynn'nnrTG
Cuts at: 0 286 831
Size: 286 545
MspA1I CmG'CkG
Cuts at: 0 274 610 831
Size: 274 336 221
Fragments arranged by size:
336 274 221
NcoI C'CATG_G
Cuts at: 0 506 831
Size: 506 325
PflMI CCAn_nnn'nTGG
Cuts at: 0 31 819 831
Size: 31 788 12
Fragments arranged by size:
788 31 12
PstI C_TGCA'G
Cuts at: 0 279 759 831
Size: 279 480 72
Fragments arranged by size:
480 279 72
PvuII CAG'CTG
Cuts at: 0 274 610 831
Size: 274 336 221
Fragments arranged by size:
336 274 221
SacI G_AGCT'C
Cuts at: 0 323 831
Size: 323 508
SfcI C'TryA_G
Cuts at: 0 275 459 755 831
Size: 275 184 296 76
Fragments arranged by size:
296 275 184 76
SspI AAT'ATT
Cuts at: 0 249 831
Size: 249 582
StyI C'CwwG_G
Cuts at: 0 506 521 831
Size: 506 15 310
Fragments arranged by size:
506 310 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 316 348 831
Size: 316 32 483
Fragments arranged by size:
483 316 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 141 831
Size: 141 690
Enzymes that do cut:
AcuI BaeI BanII BbsI BcgI BciVI Bme1580I BplI
BpmI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspEI BsrDI BsrFI BstXI BtgI BtgZI DrdI
EaeI EciI EcoICRI Eco57MI Hin4I HindIII MmeI MslI
MspA1I NcoI PflMI PstI PvuII SacI SfcI SspI
StyI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI AvrII BamHI BanI
BbeI BbvCI BclI BfrBI BglI BglII BlpI BmgBI
BmrI BmtI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsiEI BsiWI BsmBI BspHI BspMI BsrBI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtsI
ClaI CspCI DraI DraIII EagI EarI EcoNI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII
HpaI KasI KpnI MfeI MluI MscI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI StuI
SwaI TaqII TatI Tth111I XbaI XhoI XmaI XmnI
ZraI