Restriction map for
pF1KB8271
(Linear) (Six-base) MAPSORT of: KIBB8271.seq Check: 3391 from: 1 to: 951
KIBB8271.orf
With 182 enzymes: SgfI *
March 3, 2008 11:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 180 482 747 951
Size: 180 302 265 204
Fragments arranged by size:
302 265 204 180
AfeI AGC'GCT
Cuts at: 0 127 951
Size: 127 824
AflIII A'CryG_T
Cuts at: 0 383 951
Size: 383 568
AlwNI CAG_nnn'CTG
Cuts at: 0 180 231 951
Size: 180 51 720
Fragments arranged by size:
720 180 51
ApaI G_GGCC'C
Cuts at: 0 552 602 675 951
Size: 552 50 73 276
Fragments arranged by size:
552 276 73 50
AvaI C'yCGr_G
Cuts at: 0 88 951
Size: 88 863
BanI G'GyrC_C
Cuts at: 0 559 916 951
Size: 559 357 35
Fragments arranged by size:
559 357 35
BanII G_rGCy'C
Cuts at: 0 89 552 602 643 675 854 951
Size: 89 463 50 41 32 179 97
Fragments arranged by size:
463 179 97 89 50 41 32
BbeI G_GCGC'C
Cuts at: 0 563 951
Size: 563 388
BbsI GAAGACnn'nnnn_
Cuts at: 0 869 951
Size: 869 82
BbvCI CC'TCA_GC
Cuts at: 0 574 951
Size: 574 377
BglI GCCn_nnn'nGGC
Cuts at: 0 826 951
Size: 826 125
BlpI GC'TnA_GC
Cuts at: 0 208 951
Size: 208 743
Bme1580I G_kGCm'C
Cuts at: 0 327 552 602 675 919 938 951
Size: 327 225 50 73 244 19 13
Fragments arranged by size:
327 244 225 73 50 19 13
BmgBI CAC'GTC
Cuts at: 0 384 951
Size: 384 567
BmrI ACTGGGnnnn_n'
Cuts at: 0 151 951
Size: 151 800
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 802 951
Size: 307 495 149
Fragments arranged by size:
495 307 149
Bpu10I CC'TnA_GC
Cuts at: 0 574 951
Size: 574 377
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 820 951
Size: 820 131
BsaI GGTCTCn'nnnn_
Cuts at: 0 244 951
Size: 244 707
BsaHI Gr'CG_yC
Cuts at: 0 560 951
Size: 560 391
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 370 400 951
Size: 370 30 551
Fragments arranged by size:
551 370 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 214 328 460 487 724 799 951
Size: 214 114 132 27 237 75 152
Fragments arranged by size:
237 214 152 132 114 75 27
BseYI C'CCAG_C
Cuts at: 0 128 167 363 794 937 951
Size: 128 39 196 431 143 14
Fragments arranged by size:
431 196 143 128 39 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 193 214 421 439 922 951
Size: 193 21 207 18 483 29
Fragments arranged by size:
483 207 193 29 21 18
BsiEI CG_ry'CG
Cuts at: 0 393 735 951
Size: 393 342 216
Fragments arranged by size:
393 342 216
BsiHKAI G_wGCw'C
Cuts at: 0 442 951
Size: 442 509
BsmBI CGTCTCn'nnnn_
Cuts at: 0 310 951
Size: 310 641
Bsp1286I G_dGCh'C
Cuts at: 0 89 327 442 552 602 643 675 854
Size: 89 238 115 110 50 41 32 179
Cuts at: 854 919 938 951
Size: 65 19 13
Fragments arranged by size:
238 179 115 110 89 65 50 41
32 19 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 170 180 482 524 747 951
Size: 170 10 302 42 223 204
Fragments arranged by size:
302 223 204 170 42 10
BsrBI CCG'CTC
Cuts at: 0 119 669 690 951
Size: 119 550 21 261
Fragments arranged by size:
550 261 119 21
BsrFI r'CCGG_y
Cuts at: 0 649 951
Size: 649 302
BssHII G'CGCG_C
Cuts at: 0 696 698 771 951
Size: 696 2 73 180
Fragments arranged by size:
696 180 73 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 426 951
Size: 426 525
BstEII G'GTnAC_C
Cuts at: 0 219 951
Size: 219 732
BtgI C'CryG_G
Cuts at: 0 408 911 951
Size: 408 503 40
Fragments arranged by size:
503 408 40
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 518 951
Size: 518 433
BtsI GCAGTG_nn'
Cuts at: 0 539 951
Size: 539 412
EaeI y'GGCC_r
Cuts at: 0 120 390 432 647 651 951
Size: 120 270 42 215 4 300
Fragments arranged by size:
300 270 215 120 42 4
EagI C'GGCC_G
Cuts at: 0 390 951
Size: 390 561
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 325 778 951
Size: 325 453 173
Fragments arranged by size:
453 325 173
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 802 951
Size: 307 495 149
Fragments arranged by size:
495 307 149
EcoNI CCTnn'n_nnAGG
Cuts at: 0 742 951
Size: 742 209
EcoO109I rG'GnC_Cy
Cuts at: 0 548 598 599 951
Size: 548 50 1 352
Fragments arranged by size:
548 352 50 1
FseI GG_CCGG'CC
Cuts at: 0 653 951
Size: 653 298
FspI TGC'GCA
Cuts at: 0 478 951
Size: 478 473
HaeII r_GCGC'y
Cuts at: 0 129 241 528 563 581 594 876 926
Size: 129 112 287 35 18 13 282 50
Cuts at: 926 951
Size: 25
Fragments arranged by size:
287 282 129 112 50 35 25 18
13
KasI G'GCGC_C
Cuts at: 0 559 951
Size: 559 392
MspA1I CmG'CkG
Cuts at: 0 410 951
Size: 410 541
NaeI GCC'GGC
Cuts at: 0 651 951
Size: 651 300
NarI GG'CG_CC
Cuts at: 0 560 951
Size: 560 391
NgoMIV G'CCGG_C
Cuts at: 0 649 951
Size: 649 302
NotI GC'GGCC_GC
Cuts at: 0 390 951
Size: 390 561
NspI r_CATG'y
Cuts at: 0 870 951
Size: 870 81
PspOMI G'GGCC_C
Cuts at: 0 548 598 671 951
Size: 548 50 73 280
Fragments arranged by size:
548 280 73 50
PstI C_TGCA'G
Cuts at: 0 347 521 812 951
Size: 347 174 291 139
Fragments arranged by size:
347 291 174 139
SacII CC_GC'GG
Cuts at: 0 411 951
Size: 411 540
SbfI CC_TGCA'GG
Cuts at: 0 812 951
Size: 812 139
SfcI C'TryA_G
Cuts at: 0 343 517 661 808 951
Size: 343 174 144 147 143
Fragments arranged by size:
343 174 147 144 143
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 826 951
Size: 826 125
SfoI GGC'GCC
Cuts at: 0 561 951
Size: 561 390
SmlI C'TyrA_G
Cuts at: 0 835 951
Size: 835 116
StuI AGG'CCT
Cuts at: 0 271 679 820 951
Size: 271 408 141 131
Fragments arranged by size:
408 271 141 131
StyI C'CwwG_G
Cuts at: 0 53 335 951
Size: 53 282 616
Fragments arranged by size:
616 282 53
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 121 159 238 951
Size: 121 38 79 713
Fragments arranged by size:
713 121 79 38
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 370 402 951
Size: 370 32 549
Fragments arranged by size:
549 370 32
Tth111I GACn'n_nGTC
Cuts at: 0 180 951
Size: 180 771
Enzymes that do cut:
AarI AfeI AflIII AlwNI ApaI AvaI BanI BanII
BbeI BbsI BbvCI BglI BlpI Bme1580I BmgBI BmrI
BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI
BssHII BstAPI BstEII BtgI BtgZI BtsI EaeI EagI
EciI Eco57MI EcoNI EcoO109I FseI FspI HaeII KasI
MspA1I NaeI NarI NgoMIV NotI NspI PspOMI PstI
SacII SbfI SfcI SfiI SfoI SmlI StuI StyI
TaqII TstI Tth111I
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BcgI BciVI BclI BfrBI BglII
BmtI BplI BsaAI BsaBI BsaWI BsiWI BsmI BspEI
BspHI BsrDI BsrGI BssSI BstBI BstXI BstYI BstZ17I
Bsu36I ClaI CspCI DraI DraIII DrdI EarI EcoICRI
EcoRI EcoRV FalI FspAI Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI NcoI NdeI
NheI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI PvuII RsrII SacI SalI SanDI SapI ScaI
SexAI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI TatI XbaI XcmI XhoI XmaI XmnI
ZraI