Restriction map for
pF1KB8267
(Linear) (Six-base) MAPSORT of: KIBB8267.seq Check: 9946 from: 1 to: 693
KIBB8267.orf
With 182 enzymes: SgfI *
March 3, 2008 11:04 ..
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 40 43 693
Size: 40 3 650
Fragments arranged by size:
650 40 3
AlwNI CAG_nnn'CTG
Cuts at: 0 457 466 693
Size: 457 9 227
Fragments arranged by size:
457 227 9
ApaI G_GGCC'C
Cuts at: 0 310 571 693
Size: 310 261 122
Fragments arranged by size:
310 261 122
ApoI r'AATT_y
Cuts at: 0 114 693
Size: 114 579
AvaI C'yCGr_G
Cuts at: 0 278 693
Size: 278 415
BamHI G'GATC_C
Cuts at: 0 611 693
Size: 611 82
BanI G'GyrC_C
Cuts at: 0 85 693
Size: 85 608
BanII G_rGCy'C
Cuts at: 0 310 492 571 693
Size: 310 182 79 122
Fragments arranged by size:
310 182 122 79
Bme1580I G_kGCm'C
Cuts at: 0 310 571 693
Size: 310 261 122
Fragments arranged by size:
310 261 122
BmrI ACTGGGnnnn_n'
Cuts at: 0 492 693
Size: 492 201
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 314 539 693
Size: 314 225 154
Fragments arranged by size:
314 225 154
BsaI GGTCTCn'nnnn_
Cuts at: 0 237 579 693
Size: 237 342 114
Fragments arranged by size:
342 237 114
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 245 557 656 693
Size: 245 312 99 37
Fragments arranged by size:
312 245 99 37
BseYI C'CCAG_C
Cuts at: 0 342 346 382 693
Size: 342 4 36 311
Fragments arranged by size:
342 311 36 4
BsiEI CG_ry'CG
Cuts at: 0 72 693
Size: 72 621
BsiHKAI G_wGCw'C
Cuts at: 0 549 693
Size: 549 144
BsmI GAATG_Cn'
Cuts at: 0 662 693
Size: 662 31
Bsp1286I G_dGCh'C
Cuts at: 0 310 492 549 571 693
Size: 310 182 57 22 122
Fragments arranged by size:
310 182 122 57 22
BstAPI GCAn_nnn'nTGC
Cuts at: 0 379 457 466 693
Size: 379 78 9 227
Fragments arranged by size:
379 227 78 9
BstYI r'GATC_y
Cuts at: 0 74 611 693
Size: 74 537 82
Fragments arranged by size:
537 82 74
BtgI C'CryG_G
Cuts at: 0 393 513 693
Size: 393 120 180
Fragments arranged by size:
393 180 120
EaeI y'GGCC_r
Cuts at: 0 69 510 693
Size: 69 441 183
Fragments arranged by size:
441 183 69
EagI C'GGCC_G
Cuts at: 0 69 693
Size: 69 624
EarI CTCTTCn'nnn_
Cuts at: 0 179 432 618 693
Size: 179 253 186 75
Fragments arranged by size:
253 186 179 75
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 314 539 693
Size: 314 225 154
Fragments arranged by size:
314 225 154
EcoNI CCTnn'n_nnAGG
Cuts at: 0 623 693
Size: 623 70
EcoO109I rG'GnC_Cy
Cuts at: 0 250 306 520 598 693
Size: 250 56 214 78 95
Fragments arranged by size:
250 214 95 78 56
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 517 693
Size: 517 176
MspA1I CmG'CkG
Cuts at: 0 346 457 463 693
Size: 346 111 6 230
Fragments arranged by size:
346 230 111 6
NspI r_CATG'y
Cuts at: 0 645 693
Size: 645 48
PasI CC'CwG_GG
Cuts at: 0 310 673 693
Size: 310 363 20
Fragments arranged by size:
363 310 20
PflMI CCAn_nnn'nTGG
Cuts at: 0 292 394 577 693
Size: 292 102 183 116
Fragments arranged by size:
292 183 116 102
PfoI T'CCnGG_A
Cuts at: 0 651 693
Size: 651 42
PpuMI rG'GwC_Cy
Cuts at: 0 250 520 598 693
Size: 250 270 78 95
Fragments arranged by size:
270 250 95 78
PspOMI G'GGCC_C
Cuts at: 0 306 567 693
Size: 306 261 126
Fragments arranged by size:
306 261 126
PspXI vC'TCGA_Gb
Cuts at: 0 278 693
Size: 278 415
PstI C_TGCA'G
Cuts at: 0 32 65 375 462 693
Size: 32 33 310 87 231
Fragments arranged by size:
310 231 87 33 32
PvuII CAG'CTG
Cuts at: 0 346 463 693
Size: 346 117 230
Fragments arranged by size:
346 230 117
SanDI GG'GwC_CC
Cuts at: 0 598 693
Size: 598 95
SbfI CC_TGCA'GG
Cuts at: 0 65 375 693
Size: 65 310 318
Fragments arranged by size:
318 310 65
SfcI C'TryA_G
Cuts at: 0 28 61 371 458 693
Size: 28 33 310 87 235
Fragments arranged by size:
310 235 87 33 28
SmlI C'TyrA_G
Cuts at: 0 278 693
Size: 278 415
SphI G_CATG'C
Cuts at: 0 645 693
Size: 645 48
StuI AGG'CCT
Cuts at: 0 686 693
Size: 686 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 190 693
Size: 190 503
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 505 693
Size: 505 188
XhoI C'TCGA_G
Cuts at: 0 278 693
Size: 278 415
Enzymes that do cut:
AlfI AlwNI ApaI ApoI AvaI BamHI BanI BanII
Bme1580I BmrI BpmI BsaI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BstAPI BstYI BtgI EaeI EagI EarI
Eco57MI EcoNI EcoO109I MmeI MspA1I NspI PasI PflMI
PfoI PpuMI PspOMI PspXI PstI PvuII SanDI SbfI
SfcI SmlI SphI StuI TaqII XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AloI ApaLI AscI AseI
AsiSI AvrII BaeI BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmtI BplI
Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BsgI
BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EciI
EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MscI MslI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PciI PmeI PmlI PpiI
PshAI PsiI PsrI PvuI RsrII SacI SacII SalI
SapI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI StyI SwaI TatI TstI
Tth111I XbaI XmaI XmnI ZraI