Restriction map for
pF1KB8247
(Linear) (Six-base) MAPSORT of: KIBB8247.seq Check: 5602 from: 1 to: 771
KIBB8247.orf
With 182 enzymes: SgfI *
March 3, 2008 11:04 ..
AccI GT'mk_AC
Cuts at: 0 72 392 771
Size: 72 320 379
Fragments arranged by size:
379 320 72
AfeI AGC'GCT
Cuts at: 0 217 771
Size: 217 554
ApaI G_GGCC'C
Cuts at: 0 177 655 771
Size: 177 478 116
Fragments arranged by size:
478 177 116
AvaI C'yCGr_G
Cuts at: 0 268 771
Size: 268 503
BamHI G'GATC_C
Cuts at: 0 587 771
Size: 587 184
BanII G_rGCy'C
Cuts at: 0 98 177 655 771
Size: 98 79 478 116
Fragments arranged by size:
478 116 98 79
BbsI GAAGACnn'nnnn_
Cuts at: 0 704 771
Size: 704 67
Bme1580I G_kGCm'C
Cuts at: 0 177 655 771
Size: 177 478 116
Fragments arranged by size:
478 177 116
BmrI ACTGGGnnnn_n'
Cuts at: 0 400 771
Size: 400 371
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 184 436 771
Size: 112 72 252 335
Fragments arranged by size:
335 252 112 72
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 771
Size: 61 710
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 190 220 771
Size: 190 30 551
Fragments arranged by size:
551 190 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 298 771
Size: 298 473
BseYI C'CCAG_C
Cuts at: 0 66 227 535 771
Size: 66 161 308 236
Fragments arranged by size:
308 236 161 66
BsiHKAI G_wGCw'C
Cuts at: 0 98 771
Size: 98 673
Bsp1286I G_dGCh'C
Cuts at: 0 98 177 655 771
Size: 98 79 478 116
Fragments arranged by size:
478 116 98 79
BsrBI CCG'CTC
Cuts at: 0 360 771
Size: 360 411
BstXI CCAn_nnnn'nTGG
Cuts at: 0 723 771
Size: 723 48
BstYI r'GATC_y
Cuts at: 0 587 771
Size: 587 184
DraIII CAC_nnn'GTG
Cuts at: 0 408 771
Size: 408 363
EaeI y'GGCC_r
Cuts at: 0 458 725 771
Size: 458 267 46
Fragments arranged by size:
458 267 46
EarI CTCTTCn'nnn_
Cuts at: 0 56 316 598 771
Size: 56 260 282 173
Fragments arranged by size:
282 260 173 56
EcoICRI GAG'CTC
Cuts at: 0 96 771
Size: 96 675
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 184 436 771
Size: 112 72 252 335
Fragments arranged by size:
335 252 112 72
EcoNI CCTnn'n_nnAGG
Cuts at: 0 193 430 460 771
Size: 193 237 30 311
Fragments arranged by size:
311 237 193 30
EcoO109I rG'GnC_Cy
Cuts at: 0 173 645 652 771
Size: 173 472 7 119
Fragments arranged by size:
472 173 119 7
HaeII r_GCGC'y
Cuts at: 0 219 771
Size: 219 552
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 190 222 629 661 771
Size: 190 32 407 32 110
Fragments arranged by size:
407 190 110 32 32
HincII GTy'rAC
Cuts at: 0 73 771
Size: 73 698
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 335 771
Size: 335 436
MscI TGG'CCA
Cuts at: 0 460 727 771
Size: 460 267 44
Fragments arranged by size:
460 267 44
MspA1I CmG'CkG
Cuts at: 0 756 771
Size: 756 15
PflMI CCAn_nnn'nTGG
Cuts at: 0 294 771
Size: 294 477
PpuMI rG'GwC_Cy
Cuts at: 0 645 771
Size: 645 126
PshAI GACnn'nnGTC
Cuts at: 0 388 771
Size: 388 383
PspOMI G'GGCC_C
Cuts at: 0 173 651 771
Size: 173 478 120
Fragments arranged by size:
478 173 120
PvuII CAG'CTG
Cuts at: 0 756 771
Size: 756 15
SacI G_AGCT'C
Cuts at: 0 98 771
Size: 98 673
SalI G'TCGA_C
Cuts at: 0 71 771
Size: 71 700
SapI GCTCTTCn'nnn_
Cuts at: 0 56 771
Size: 56 715
SfcI C'TryA_G
Cuts at: 0 666 771
Size: 666 105
SmlI C'TyrA_G
Cuts at: 0 13 76 771
Size: 13 63 695
Fragments arranged by size:
695 63 13
StuI AGG'CCT
Cuts at: 0 553 771
Size: 553 218
StyI C'CwwG_G
Cuts at: 0 371 461 771
Size: 371 90 310
Fragments arranged by size:
371 310 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 419 527 621 771
Size: 419 108 94 150
Fragments arranged by size:
419 150 108 94
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 394 426 693 725 771
Size: 394 32 267 32 46
Fragments arranged by size:
394 267 46 32 32
Enzymes that do cut:
AccI AfeI ApaI AvaI BamHI BanII BbsI Bme1580I
BmrI BpmI BpuEI BsaXI BseRI BseYI BsiHKAI Bsp1286I
BsrBI BstXI BstYI DraIII EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I HaeII Hin4I HincII MmeI MscI MspA1I
PflMI PpuMI PshAI PspOMI PvuII SacI SalI SapI
SfcI SmlI StuI StyI TaqII TstI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BanI BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI BmgBI BmtI
BplI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsgI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EagI EciI EcoRI EcoRV FalI FseI FspI FspAI
HindIII HpaI KasI KpnI MfeI MluI MslI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PfoI PmeI PmlI PpiI
PsiI PspXI PsrI PstI PvuI RsrII SacII SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI