Restriction map for
pF1KB8246
(Linear) (Six-base) MAPSORT of: KIBB8246.seq Check: 140 from: 1 to: 735
KIBB8246.orf
With 182 enzymes: SgfI *
March 3, 2008 11:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 334 735
Size: 334 401
Acc65I G'GTAC_C
Cuts at: 0 213 735
Size: 213 522
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 396 444 735
Size: 396 48 291
Fragments arranged by size:
396 291 48
AflIII A'CryG_T
Cuts at: 0 149 735
Size: 149 586
AlwNI CAG_nnn'CTG
Cuts at: 0 240 735
Size: 240 495
ApaLI G'TGCA_C
Cuts at: 0 145 735
Size: 145 590
ApoI r'AATT_y
Cuts at: 0 380 735
Size: 380 355
BanI G'GyrC_C
Cuts at: 0 213 735
Size: 213 522
BanII G_rGCy'C
Cuts at: 0 125 735
Size: 125 610
BbsI GAAGACnn'nnnn_
Cuts at: 0 402 450 509 654 735
Size: 402 48 59 145 81
Fragments arranged by size:
402 145 81 59 48
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 232 266 735
Size: 232 34 469
Fragments arranged by size:
469 232 34
Bme1580I G_kGCm'C
Cuts at: 0 149 735
Size: 149 586
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 318 537 735
Size: 318 219 198
Fragments arranged by size:
318 219 198
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 382 735
Size: 382 353
BsaI GGTCTCn'nnnn_
Cuts at: 0 211 735
Size: 211 524
BsaAI yAC'GTr
Cuts at: 0 339 735
Size: 339 396
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 464 494 735
Size: 464 30 241
Fragments arranged by size:
464 241 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 307 735
Size: 307 428
BseYI C'CCAG_C
Cuts at: 0 583 680 735
Size: 583 97 55
Fragments arranged by size:
583 97 55
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 735
Size: 295 440
BsiEI CG_ry'CG
Cuts at: 0 83 735
Size: 83 652
BsiHKAI G_wGCw'C
Cuts at: 0 125 149 398 735
Size: 125 24 249 337
Fragments arranged by size:
337 249 125 24
Bsp1286I G_dGCh'C
Cuts at: 0 125 149 398 735
Size: 125 24 249 337
Fragments arranged by size:
337 249 125 24
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 334 735
Size: 334 401
BsrBI CCG'CTC
Cuts at: 0 297 735
Size: 297 438
BsrDI GCAATG_nn'
Cuts at: 0 321 336 692 735
Size: 321 15 356 43
Fragments arranged by size:
356 321 43 15
BsrFI r'CCGG_y
Cuts at: 0 160 735
Size: 160 575
BstXI CCAn_nnnn'nTGG
Cuts at: 0 141 735
Size: 141 594
BtgI C'CryG_G
Cuts at: 0 140 735
Size: 140 595
BtsI GCAGTG_nn'
Cuts at: 0 573 735
Size: 573 162
EaeI y'GGCC_r
Cuts at: 0 425 735
Size: 425 310
EarI CTCTTCn'nnn_
Cuts at: 0 530 735
Size: 530 205
EcoICRI GAG'CTC
Cuts at: 0 123 735
Size: 123 612
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 396 444 735
Size: 396 48 291
Fragments arranged by size:
396 291 48
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 556 735
Size: 556 179
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 502 534 735
Size: 502 32 201
Fragments arranged by size:
502 201 32
KpnI G_GTAC'C
Cuts at: 0 217 735
Size: 217 518
MfeI C'AATT_G
Cuts at: 0 497 735
Size: 497 238
MluI A'CGCG_T
Cuts at: 0 149 735
Size: 149 586
MslI CAynn'nnrTG
Cuts at: 0 139 187 703 735
Size: 139 48 516 32
Fragments arranged by size:
516 139 48 32
NaeI GCC'GGC
Cuts at: 0 162 735
Size: 162 573
NgoMIV G'CCGG_C
Cuts at: 0 160 735
Size: 160 575
NspI r_CATG'y
Cuts at: 0 186 708 735
Size: 186 522 27
Fragments arranged by size:
522 186 27
PvuI CG_AT'CG
Cuts at: 0 83 735
Size: 83 652
RsrII CG'GwC_CG
Cuts at: 0 6 735
Size: 6 729
SacI G_AGCT'C
Cuts at: 0 125 735
Size: 125 610
SexAI A'CCwGG_T
Cuts at: 0 431 735
Size: 431 304
SfcI C'TryA_G
Cuts at: 0 102 735
Size: 102 633
SmlI C'TyrA_G
Cuts at: 0 397 735
Size: 397 338
SphI G_CATG'C
Cuts at: 0 708 735
Size: 708 27
StyI C'CwwG_G
Cuts at: 0 308 344 735
Size: 308 36 391
Fragments arranged by size:
391 308 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 368 735
Size: 368 367
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 464 496 735
Size: 464 32 239
Fragments arranged by size:
464 239 32
XmnI GAAnn'nnTTC
Cuts at: 0 380 735
Size: 380 355
Enzymes that do cut:
AarI Acc65I AcuI AflIII AlwNI ApaLI ApoI BanI
BanII BbsI BcgI Bme1580I BplI BpuEI BsaI BsaAI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI
BsrBI BsrDI BsrFI BstXI BtgI BtsI EaeI EarI
EcoICRI Eco57MI FalI Hin4I KpnI MfeI MluI MslI
NaeI NgoMIV NspI PvuI RsrII SacI SexAI SfcI
SmlI SphI StyI TaqII TstI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BbvCI BciVI BclI BfrBI BglI
BglII BlpI BmgBI BmrI BmtI BpmI Bpu10I BsaBI
BsaHI BsaWI BsiWI BsmI BsmBI BspEI BspHI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI HaeII
HincII HindIII HpaI KasI MmeI MscI MspA1I NarI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuII SacII SalI
SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI StuI SwaI TatI
Tth111I XbaI XcmI XhoI XmaI ZraI