Restriction map for
pF1KB8231
(Linear) (Six-base) MAPSORT of: KIBB8231.seq Check: 272 from: 1 to: 633
KIBB8231.orf
With 182 enzymes: SgfI *
March 3, 2008 11:04 ..
Acc65I G'GTAC_C
Cuts at: 0 335 633
Size: 335 298
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 633
Size: 229 404
AlwNI CAG_nnn'CTG
Cuts at: 0 126 570 633
Size: 126 444 63
Fragments arranged by size:
444 126 63
ApaI G_GGCC'C
Cuts at: 0 211 633
Size: 211 422
AvaI C'yCGr_G
Cuts at: 0 167 633
Size: 167 466
BamHI G'GATC_C
Cuts at: 0 624 633
Size: 624 9
BanI G'GyrC_C
Cuts at: 0 335 633
Size: 335 298
BanII G_rGCy'C
Cuts at: 0 211 234 633
Size: 211 23 399
Fragments arranged by size:
399 211 23
Bme1580I G_kGCm'C
Cuts at: 0 211 633
Size: 211 422
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 50 317 633
Size: 50 267 316
Fragments arranged by size:
316 267 50
BsaI GGTCTCn'nnnn_
Cuts at: 0 9 21 108 633
Size: 9 12 87 525
Fragments arranged by size:
525 87 12 9
BsaBI GATnn'nnATC
Cuts at: 0 623 633
Size: 623 10
BsaWI w'CCGG_w
Cuts at: 0 453 633
Size: 453 180
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 533 552 633
Size: 533 19 81
Fragments arranged by size:
533 81 19
BsiHKAI G_wGCw'C
Cuts at: 0 234 633
Size: 234 399
BsmBI CGTCTCn'nnnn_
Cuts at: 0 519 633
Size: 519 114
Bsp1286I G_dGCh'C
Cuts at: 0 211 234 633
Size: 211 23 399
Fragments arranged by size:
399 211 23
BspEI T'CCGG_A
Cuts at: 0 453 633
Size: 453 180
BspHI T'CATG_A
Cuts at: 0 234 633
Size: 234 399
BsrDI GCAATG_nn'
Cuts at: 0 155 633
Size: 155 478
BstXI CCAn_nnnn'nTGG
Cuts at: 0 282 633
Size: 282 351
BstYI r'GATC_y
Cuts at: 0 624 633
Size: 624 9
Bsu36I CC'TnA_GG
Cuts at: 0 311 633
Size: 311 322
BtsI GCAGTG_nn'
Cuts at: 0 404 633
Size: 404 229
EcoICRI GAG'CTC
Cuts at: 0 232 633
Size: 232 401
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 50 229 317 633
Size: 50 179 88 316
Fragments arranged by size:
316 179 88 50
EcoO109I rG'GnC_Cy
Cuts at: 0 164 208 256 633
Size: 164 44 48 377
Fragments arranged by size:
377 164 48 44
EcoRV GAT'ATC
Cuts at: 0 117 633
Size: 117 516
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 88 120 531 563 633
Size: 88 32 411 32 70
Fragments arranged by size:
411 88 70 32 32
HincII GTy'rAC
Cuts at: 0 293 633
Size: 293 340
KpnI G_GTAC'C
Cuts at: 0 339 633
Size: 339 294
MfeI C'AATT_G
Cuts at: 0 148 633
Size: 148 485
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 345 633
Size: 345 288
MslI CAynn'nnrTG
Cuts at: 0 57 633
Size: 57 576
PfoI T'CCnGG_A
Cuts at: 0 7 633
Size: 7 626
PpuMI rG'GwC_Cy
Cuts at: 0 256 633
Size: 256 377
PspOMI G'GGCC_C
Cuts at: 0 207 633
Size: 207 426
PstI C_TGCA'G
Cuts at: 0 411 633
Size: 411 222
SacI G_AGCT'C
Cuts at: 0 234 633
Size: 234 399
SfcI C'TryA_G
Cuts at: 0 407 633
Size: 407 226
Tth111I GACn'n_nGTC
Cuts at: 0 100 633
Size: 100 533
XbaI T'CTAG_A
Cuts at: 0 387 633
Size: 387 246
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 168 201 633
Size: 168 33 432
Fragments arranged by size:
432 168 33
XmnI GAAnn'nnTTC
Cuts at: 0 242 633
Size: 242 391
Enzymes that do cut:
Acc65I AcuI AlwNI ApaI AvaI BamHI BanI BanII
Bme1580I BpmI BsaI BsaBI BsaWI BseRI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BsrDI BstXI BstYI Bsu36I BtsI
EcoICRI Eco57MI EcoO109I EcoRV Hin4I HincII KpnI MfeI
MmeI MslI PfoI PpuMI PspOMI PstI SacI SfcI
Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI
BplI Bpu10I BpuEI BsaAI BsaHI BsaXI BseYI BsgI
BsiEI BsiWI BsmI BspMI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstZ17I BtgI BtgZI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoNI EcoRI FalI FseI FspI FspAI HaeII HindIII
HpaI KasI MluI MscI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI SwaI TaqII TatI TstI XhoI XmaI ZraI