Restriction map for
pF1KB8220
(Linear) (Six-base) MAPSORT of: KIBB8220.seq Check: 3422 from: 1 to: 1296
KIBB8220.orf
With 182 enzymes: SgfI *
March 3, 2008 11:04 ..
AatII G_ACGT'C
Cuts at: 0 344 1296
Size: 344 952
AccI GT'mk_AC
Cuts at: 0 344 1296
Size: 344 952
Acc65I G'GTAC_C
Cuts at: 0 767 1296
Size: 767 529
AhdI GACnn_n'nnGTC
Cuts at: 0 655 888 1188 1296
Size: 655 233 300 108
Fragments arranged by size:
655 300 233 108
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 577 609 1296
Size: 577 32 687
Fragments arranged by size:
687 577 32
AlwNI CAG_nnn'CTG
Cuts at: 0 441 799 1053 1296
Size: 441 358 254 243
Fragments arranged by size:
441 358 254 243
AvaI C'yCGr_G
Cuts at: 0 143 194 1296
Size: 143 51 1102
Fragments arranged by size:
1102 143 51
BanI G'GyrC_C
Cuts at: 0 100 767 857 1296
Size: 100 667 90 439
Fragments arranged by size:
667 439 100 90
BanII G_rGCy'C
Cuts at: 0 227 326 1296
Size: 227 99 970
Fragments arranged by size:
970 227 99
BbeI G_GCGC'C
Cuts at: 0 861 1296
Size: 861 435
BbsI GAAGACnn'nnnn_
Cuts at: 0 1237 1296
Size: 1237 59
BfrBI ATG'CAT
Cuts at: 0 750 1296
Size: 750 546
BglI GCCn_nnn'nGGC
Cuts at: 0 541 1296
Size: 541 755
BlpI GC'TnA_GC
Cuts at: 0 296 353 1296
Size: 296 57 943
Fragments arranged by size:
943 296 57
Bme1580I G_kGCm'C
Cuts at: 0 171 1296
Size: 171 1125
BmgBI CAC'GTC
Cuts at: 0 675 1296
Size: 675 621
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 430 448 577 598 616 907 937 1012
Size: 430 18 129 21 18 291 30 75
Cuts at: 1012 1296
Size: 284
Fragments arranged by size:
430 291 284 129 75 30 21 18
18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 618 997 1195 1296
Size: 618 379 198 101
Fragments arranged by size:
618 379 198 101
BsaI GGTCTCn'nnnn_
Cuts at: 0 184 1296
Size: 184 1112
BsaAI yAC'GTr
Cuts at: 0 951 1296
Size: 951 345
BsaHI Gr'CG_yC
Cuts at: 0 341 736 858 1296
Size: 341 395 122 438
Fragments arranged by size:
438 395 341 122
BsaWI w'CCGG_w
Cuts at: 0 590 1124 1296
Size: 590 534 172
Fragments arranged by size:
590 534 172
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 577 607 1296
Size: 577 30 689
Fragments arranged by size:
689 577 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 21 36 595 598 628 631 955 1132
Size: 21 15 559 3 30 3 324 177
Cuts at: 1132 1296
Size: 164
Fragments arranged by size:
559 324 177 164 30 21 15 3
3
BseYI C'CCAG_C
Cuts at: 0 163 1296
Size: 163 1133
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1162 1296
Size: 1162 134
BsiHKAI G_wGCw'C
Cuts at: 0 227 1283 1296
Size: 227 1056 13
Fragments arranged by size:
1056 227 13
BsmI GAATG_Cn'
Cuts at: 0 128 1296
Size: 128 1168
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3 48 1296
Size: 3 45 1248
Fragments arranged by size:
1248 45 3
Bsp1286I G_dGCh'C
Cuts at: 0 171 227 326 1283 1296
Size: 171 56 99 957 13
Fragments arranged by size:
957 171 99 56 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 645 886 1061 1166 1296
Size: 645 241 175 105 130
Fragments arranged by size:
645 241 175 130 105
BsrBI CCG'CTC
Cuts at: 0 35 207 773 945 1296
Size: 35 172 566 172 351
Fragments arranged by size:
566 351 172 172 35
BsrDI GCAATG_nn'
Cuts at: 0 737 1296
Size: 737 559
BsrFI r'CCGG_y
Cuts at: 0 854 1296
Size: 854 442
BssSI C'ACGA_G
Cuts at: 0 610 1296
Size: 610 686
BstAPI GCAn_nnn'nTGC
Cuts at: 0 175 1296
Size: 175 1121
BstXI CCAn_nnnn'nTGG
Cuts at: 0 333 1296
Size: 333 963
BstYI r'GATC_y
Cuts at: 0 552 587 605 710 1296
Size: 552 35 18 105 586
Fragments arranged by size:
586 552 105 35 18
Bsu36I CC'TnA_GG
Cuts at: 0 48 481 1296
Size: 48 433 815
Fragments arranged by size:
815 433 48
BtgI C'CryG_G
Cuts at: 0 1235 1296
Size: 1235 61
EaeI y'GGCC_r
Cuts at: 0 81 443 677 962 1296
Size: 81 362 234 285 334
Fragments arranged by size:
362 334 285 234 81
EarI CTCTTCn'nnn_
Cuts at: 0 755 992 1296
Size: 755 237 304
Fragments arranged by size:
755 304 237
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 814 1296
Size: 814 482
EcoICRI GAG'CTC
Cuts at: 0 225 1296
Size: 225 1071
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 430 448 577 598 616 907 937 1012
Size: 430 18 129 21 18 291 30 75
Cuts at: 1012 1296
Size: 284
Fragments arranged by size:
430 291 284 129 75 30 21 18
18
EcoO109I rG'GnC_Cy
Cuts at: 0 69 94 438 1050 1296
Size: 69 25 344 612 246
Fragments arranged by size:
612 344 246 69 25
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 279 1296
Size: 279 1017
HaeII r_GCGC'y
Cuts at: 0 289 404 861 982 1296
Size: 289 115 457 121 314
Fragments arranged by size:
457 314 289 121 115
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 411 443 641 673 874 906 1174 1206
Size: 411 32 198 32 201 32 268 32
Cuts at: 1206 1296
Size: 90
Fragments arranged by size:
411 268 201 198 90 32 32 32
32
KasI G'GCGC_C
Cuts at: 0 857 1296
Size: 857 439
KpnI G_GTAC'C
Cuts at: 0 771 1296
Size: 771 525
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1178 1296
Size: 1178 118
MscI TGG'CCA
Cuts at: 0 445 679 1296
Size: 445 234 617
Fragments arranged by size:
617 445 234
MslI CAynn'nnrTG
Cuts at: 0 1138 1225 1286 1296
Size: 1138 87 61 10
Fragments arranged by size:
1138 87 61 10
MspA1I CmG'CkG
Cuts at: 0 25 309 642 1296
Size: 25 284 333 654
Fragments arranged by size:
654 333 284 25
NarI GG'CG_CC
Cuts at: 0 858 1296
Size: 858 438
NsiI A_TGCA'T
Cuts at: 0 752 1296
Size: 752 544
NspI r_CATG'y
Cuts at: 0 750 1296
Size: 750 546
PfoI T'CCnGG_A
Cuts at: 0 266 464 632 1296
Size: 266 198 168 664
Fragments arranged by size:
664 266 198 168
PmlI CAC'GTG
Cuts at: 0 951 1296
Size: 951 345
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 577 609 1296
Size: 577 32 687
Fragments arranged by size:
687 577 32
PpuMI rG'GwC_Cy
Cuts at: 0 94 438 1050 1296
Size: 94 344 612 246
Fragments arranged by size:
612 344 246 94
PstI C_TGCA'G
Cuts at: 0 1163 1296
Size: 1163 133
PvuII CAG'CTG
Cuts at: 0 309 642 1296
Size: 309 333 654
Fragments arranged by size:
654 333 309
SacI G_AGCT'C
Cuts at: 0 227 1296
Size: 227 1069
SfcI C'TryA_G
Cuts at: 0 513 1095 1159 1296
Size: 513 582 64 137
Fragments arranged by size:
582 513 137 64
SfoI GGC'GCC
Cuts at: 0 859 1296
Size: 859 437
SmaI CCC'GGG
Cuts at: 0 145 196 1296
Size: 145 51 1100
Fragments arranged by size:
1100 145 51
SmlI C'TyrA_G
Cuts at: 0 597 1012 1210 1296
Size: 597 415 198 86
Fragments arranged by size:
597 415 198 86
StyI C'CwwG_G
Cuts at: 0 317 1296
Size: 317 979
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 483 1296
Size: 483 813
XmaI C'CCGG_G
Cuts at: 0 143 194 1296
Size: 143 51 1102
Fragments arranged by size:
1102 143 51
ZraI GAC'GTC
Cuts at: 0 342 1296
Size: 342 954
Enzymes that do cut:
AatII AccI Acc65I AhdI AloI AlwNI AvaI BanI
BanII BbeI BbsI BfrBI BglI BlpI Bme1580I BmgBI
BpmI BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstXI BstYI Bsu36I BtgI
EaeI EarI EciI EcoICRI Eco57MI EcoO109I FalI HaeII
Hin4I KasI KpnI MmeI MscI MslI MspA1I NarI
NsiI NspI PfoI PmlI PpiI PpuMI PstI PvuII
SacI SfcI SfoI SmaI SmlI StyI XcmI XmaI
ZraI
Enzymes that do not cut:
AarI AclI AcuI AfeI AflII AflIII AgeI AleI
AlfI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbvCI BcgI BciVI BclI BglII BmrI
BmtI BplI Bpu10I BsaBI BsiEI BsiWI BspEI BspHI
BsrGI BssHII BstBI BstEII BstZ17I BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EagI EcoNI EcoRI EcoRV
FseI FspI FspAI HincII HindIII HpaI MfeI MluI
NaeI NcoI NdeI NgoMIV NheI NotI NruI PacI
PasI PciI PflMI PmeI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII TatI TstI Tth111I
XbaI XhoI XmnI